A5061199788- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Immune Response and Inflammation
- CAR-T cell therapy research
- Dermatology and Skin Diseases
- Systemic Lupus Erythematosus Research
- Food Allergy and Anaphylaxis Research
- Neonatal and Maternal Infections
- Advanced Fluorescence Microscopy Techniques
- Congenital heart defects research
- Eosinophilic Esophagitis
- CRISPR and Genetic Engineering
- Mast cells and histamine
- Inflammatory Bowel Disease
- Extracellular vesicles in disease
- Allergic Rhinitis and Sensitization
- Sepsis Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Helicobacter pylori-related gastroenterology studies
- Single-cell and spatial transcriptomics
- Cancer Research and Treatment
- Soft tissue tumor case studies
Qatar Airways (Qatar)
2022-2025
Hamad bin Khalifa University
2024-2025
National Institutes of Health
2007-2019
National Institute of Allergy and Infectious Diseases
2009-2019
Sidra Medical and Research Center
2016-2017
National Eye Institute
2015
Malaghan Institute of Medical Research
2005-2012
Cleveland Clinic
2007
Improving the functional avidity of effector T cells is critical in overcoming inhibitory factors within tumor microenvironment and eliciting regression. We have found that Cish, a member suppressor cytokine signaling (SOCS) family, induced by TCR stimulation CD8+ inhibits their against tumors. Genetic deletion Cish enhances expansion, avidity, polyfunctionality, resulting pronounced durable regression established Although commonly thought to block STAT5 activation, we primary molecular...
IL-4 production by leukocytes is a key regulatory event that occurs early in the type 2 immune response, which induces allergic reactions and mediates expulsion of parasites. CD4(+) T cells basophils are thought to be cell types produce during response. In this study, we assessed relative contribution both cell- basophil-IL-4 primary secondary responses Nippostrongylus brasiliensis using murine IL-4-enhanced GFP reporter system. During infection, IL-4-producing were detected systemically,...
Background Asthma and obesity are both characterized by inflammation. However, the combined impact of these conditions on inflammatory mechanisms in children has not been studied extensively. To address this gap, we investigated interaction effects asthma inflammation children. Methods The multiplex singleplex assays were used to measure levels circulating cytokines, including IL-2, IL-5, IL-10, IL-13, IL-17A, IL-22, IL-33, IFN-γ, TNF-α, adipokine leptin, plasma. study included 97 with...
The expression of interleukin-4 (IL-4) is viewed as the hallmark a Th2 lymphocyte, whereas subsequent action IL-4 and IL-13, mediated through STAT6 signaling pathway, seen prerequisite for full development immune responses to parasites allergens. G4 mice, whose gene locus contains fluorescent reporter eGFP, were used quantify number cells that develop during Nippostrongylus brasiliensis- or allergen-induced under conditions where was absent. Here, we show deletion had little impact on timing...
IFN-γ is a pathogenic cytokine involved in inflammation. Paradoxically, its deficiency exacerbates experimental autoimmune encephalomyelitis, uveitis, and arthritis. Here, we demonstrate using IFN-γ−/− mice repleted with IFN-γ+/+ NK cells that innate production of from necessary sufficient to trigger an endogenous regulatory circuit limits autoimmunity. After immunization, DCs recruited IFN-γ-producing the draining lymph node interacted them CXCR3-dependent fashion. The interaction caused...
Engineering CD8⁺ T cells to deliver interleukin 12 (IL-12) the tumor site can lead striking improvements in ability of adoptively transferred induce regression established murine cancers. We have recently shown that IL-12 triggers an acute inflammatory environment reverses dysfunctional antigen presentation by myeloid-derived within tumors and leads increase infiltration antigen-specific cells. Here, we find local delivery increased expression Fas tumor-infiltrating macrophages, dendritic...
mRNA vaccines have played a crucial role in combating the COVID-19 pandemic, but long-term dynamics of immune responses to repeated vaccination remain poorly understood. In this study, we extend our previous work on first and second dose by characterizing signatures elicited third using high-resolution temporal profiling blood transcriptomes collected daily for 9 days post-vaccination. We observed distinct patterns gene expression related interferon responses, inflammation, erythroid cell...
CARD11-associated atopy with dominant interference of NF-κB signaling (CADINS) is developed as a result heterozygous loss-of-function variants in CARD11 that function strong dominant-negative alleles. In lymphocytes, encodes scaffold protein links activation the antigen receptor downstream signaling. Patients CADINS generally experience severe atopic dermatitis, asthma, recurrent pneumonia and other upper respiratory tract infections, skin allergies to variety dietary environmental antigens....
Knowledge of the mechanisms underpinning development protective immunity conferred by mRNA vaccines is fragmentary. Here, we investigated responses to coronavirus disease 2019 (COVID-19) vaccination via high-temporal resolution blood transcriptome profiling. The first vaccine dose elicited modest interferon and adaptive immune responses, which peaked on days 2 5, respectively. second dose, in contrast, sharp day 1 interferon, inflammation, erythroid cell followed a 5 plasmablast response....
Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by the destruction of insulin-producing pancreatic β-cells immune system. Although conventional therapeutic modalities, such as insulin injection, remain mainstay, recent years have witnessed emergence novel treatment approaches encompassing immunomodulatory therapies, stem cell and β-cell transplantation, along with revolutionary gene-editing techniques. Notably, research endeavors enabled reshaping T-cell repertoire,...
Introduction Sepsis remains a major cause of mortality and morbidity in infants. In recent years, several gene marker strategies for the early identification sepsis have been proposed but only few independently validated adult cohorts applicability to infant unclear. Biomarkers assess disease severity risks shock also represent an important unmet need. Methods To elucidate characteristics driving infants, we assembled multi-transcriptomic dataset from public microarray datasets originating...
Allergic asthma develops in the mucosal tissue of small bronchi. At these sites, local cytokine production by Th2/Th17 cells is believed to be critical for development eosinophilia/neutrophilia. Using mouse trachea as a relevant model human airways, we performed advanced vivo dynamic and situ static imaging visualize individual cytokine-producing T airway mucosa define their immediate cellular environment. Upon allergen sensitization, newly recruited CD4+ formed discrete Ag-driven clusters...