A5020695448- Angiogenesis and VEGF in Cancer
- Reproductive Biology and Fertility
- Extracellular vesicles in disease
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- Connexins and lens biology
- Cerebrovascular and genetic disorders
- Single-cell and spatial transcriptomics
- Animal Ecology and Behavior Studies
- Birth, Development, and Health
- Ovarian function and disorders
- MicroRNA in disease regulation
- Neuroendocrine regulation and behavior
- Vascular Anomalies and Treatments
- Hormonal and reproductive studies
- Adipose Tissue and Metabolism
- Zebrafish Biomedical Research Applications
- Vascular Malformations and Hemangiomas
- Osteoarthritis Treatment and Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Barrier Structure and Function Studies
- Adipokines, Inflammation, and Metabolic Diseases
University of Virginia
2018-2023
University of California, Davis
2022-2023
William & Mary
2019
Williams (United States)
2019
The growth of endogenous collateral arteries that bypass arterial occlusion(s), or arteriogenesis, is a fundamental shear stress-induced adaptation with implications for treating peripheral disease (PAD). MicroRNAs are key regulators gene expression in response to injury and have strong therapeutic potential. In previous study, we identified miR-146a as candidate regulator vascular remodeling. Here, tested whether regulates in-vitro angiogenic endothelial cell behaviors, well perfusion...
Abstract During blood vessel development, endothelial cells become specified toward arterial or venous fates to generate a circulatory network that provides nutrients and oxygen to, removes metabolic waste from, all tissues. Arterial-venous specification occurs in conjunction with suppression of cell cycle progression; however, the mechanistic role state is unknown. Herein, using Cdh5-CreER T2 ;R26FUCCI2aR reporter mice, we find are enriched for FUCCI-Negative (early G1) BMP signaling, while...
The subventricular zone (SVZ) is the largest neural stem cell (NSC) niche in adult brain; herein, blood-brain barrier leaky, allowing direct interactions between NSCs and endothelial cells (ECs). Mechanisms by which NSC-EC SVZ control NSC behavior are unclear. We found that Cx43 highly expressed ECs, its deletion either leads to increased proliferation neuroblast generation, suggesting Cx43-mediated maintain quiescence. This further supported single-cell RNA sequencing vitro studies showing...
Distinct endothelial cell cycle states (early G1 versus late G1) provide different "windows of opportunity" to enable the differential expression genes that regulate venous arterial specification, respectively. Endothelial control and arteriovenous identities are disrupted in vascular malformations including shunts, hallmark hereditary hemorrhagic telangiectasia (HHT). To date, mechanistic link between regulation development (AVMs) HHT is not known.
Arteriogenesis, the growth of endogenous collateral arteries bypassing arterial occlusion(s), is a fundamental shear stress-induced adaptation with implications for treating peripheral disease (PAD). Nonetheless, endothelial mechano-signaling during arteriogenesis incompletely understood. Here we tested hypothesis that mechanosensitive microRNA, miR-199a-5p, regulates perfusion recovery and following femoral ligation (FAL) via control monocyte recruitment pro-arteriogenic gene expression. We...
Introduction Vascular dysfunction and chronic inflammation are characteristics of obesity-induced adipose tissue dysfunction. Proinflammatory cytokines can drive an endothelial-to-mesenchymal transition (EndoMT), where endothelial cells undergo a phenotypic switch to mesenchymal-like that pro-inflammatory pro-fibrotic. In this study, we sought determine whether obesity promote EndoMT in tissue. Methods Mice which lineage-traced with eYFP were fed high-fat/high-sucrose (HF/HS) or Control diet...
Summary Formation and maturation of a functional blood vascular system is required for the development maintenance all tissues in body. During process vessel development, primordial endothelial cells are formed become specified toward arterial or venous fates to generate circulatory network that provides nutrients oxygen to, removes metabolic waste from, 1-3 . Specification occurs conjunction with suppression cell cycle progression 4,5 , hyperproliferation associated potentially lethal...
Understanding the full process of mammalian folliculogenesis is crucial for improving assisted reproductive technologies in livestock, humans, and endangered species. Research has been mostly limited to antral large preantral follicles due difficulty isolation smaller follicles, especially mammals such as bovine This work presents an efficient approach retrieve numbers small from a single ovary. The cortex individual ovaries was sliced into 500 µm cubes using tissue chopper homogenized 6 min...
Abstract The photoperiodic response of many temperate zone rodents, including white‐footed mice ( Peromyscus leucopus ), is a heritable life‐history trait with underlying physiological variation. Previous studies intact male P. utilized two wild‐derived bidirectional selection lines, short photoperiod responsive (R) line selected for reproductive suppression in short‐day conditions (SD) and nonresponsive (NR) maturity SD. NR SD had greater food intake, but also higher levels locomotor...
Understanding the full process of mammalian folliculogenesis is crucial for improving assisted reproductive technologies in livestock, humans, and endangered species. Research has been mostly limited to antral large preantral follicles due difficulty isolation smaller follicles, especially mammals such as bovine This work presents an efficient approach retrieve numbers small from a single ovary. The cortex individual ovaries was sliced into 500 µm cubes using tissue chopper homogenized 6 min...
Abstract The subventricular zone (SVZ) is the largest neural stem cell (NSC) niche in adult brain; herein, blood-brain barrier leaky, allowing direct interactions between NSCs and endothelial cells (ECs). Mechanisms by which NSC-EC SVZ control NSC behavior are unclear. We found Cx43 to be highly expressed both ECs SVZ, its deletion either type leads increased proliferation neuroblast generation, suggesting that Cx43-mediated maintain quiescence. This further supported vitro studies showing...