Besides the effects on peripheral energy homeostasis, insulin also has an important role in ovarian function. Obesity a negative effect fertility, and may play development of polycystic ovary syndrome susceptible women. Since resistance could contribute to impairment reproductive function obese women, we evaluated signaling high-fat diet-induced rats. Female Wistar rats were submitted diet for 120 or 180 days, pathway was by immunoprecipitation immunoblotting. At end period, observed...

Identify the potential for and risk factors of SARS-CoV-2 vertical transmission.Symptomatic pregnant women with COVID-19 diagnosis in whom PCR was performed at delivery using maternal serum least one biological samples: cord blood (CB), amniotic fluid (AF), colostrum and/or oropharyngeal swab (OPS) neonate. The association parameters maternal, AF CB positivity influence on neonatal outcomes were investigated.Overall 73.4% (80/109) admitted hospital due to COVID-19, 22.9% needed intensive...

We aimed to compare sFlt-1 and placental growth factor (PlGF) levels the sFlt-1/PlGF ratio between women with preeclampsia superimposed to, respectively, normotensive chronic hypertensive ones.We performed a prospective two-armed cohort in tertiary teaching hospital Sao Paulo, Brazil, including 37 60 pregnant women. assessed serum of PlGF at 20, 26, 32, 36 gestational weeks by enzyme-linked immunosorbent assay.Having preeclampsia.Among pregnancies, 4 (10.8%) 14 (23.3%) developed...

Preeclampsia (PE) is a hypertensive disease of pregnancy-associated with placental cell death and endoplasmic reticulum (ER) stress. It unknown whether systemic factors aggravate dysfunction. We investigated serum in pregnant women PE activate ER stress unfolded protein responses (UPRs) explants trophoblast cells lineage. cultured from third-trimester term placentas control non-preeclamptic (NPE) or controls (NPE). In PE-treated explants, there was significant increase gene expression

Abstract Background Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes counter-regulating effects glucocorticoids on immune response. The conspicuous expression MIF during human implantation early embryonic development also suggests this acts in reproductive functions. overall goal study was to evaluate Mif by trophoblast embryo placental cells mouse pregnancy. Methods immunolocalized at sites gestation days...

Abstract Recent studies have highlighted the influence of fetal/maternal interactions on development asthma. Because IFN-γ reduces Th2-mediated allergic responses, we assessed its capacity to modulate asthma in offspring when injected into mothers during pregnancy. was CD1 female mice day 6.5 gestation. Immediately after birth, male newborns were housed cages with interchanged mothers: from IFN-γ-treated breastfed by normal (IFN/nor), and those (Nor/IFN) or (Nor/nor). weaning, spleen cells...

A survey of existing data suggests that trophoblast cells produce factors involved in extracellular matrix degradation. In this study, we correlated the expression cathepsins D and B murine ectoplacental cone with ultrastructural progress decidual invasion by cells. Both proteases were immunolocalized at implantation sites lysosome-endosome-like compartments giant Cathepsin D, but not cathepsin B, was also detected ultrastructurally surrounded processes invading containing components...

To determine SARS-CoV-2 seroprevalence over time and risk factors among pregnant women at delivery in São Paulo, Brazil; to evaluate the suitability of as a sentinel population for serosurveillance.

Interferon-gamma (IFN-gamma) mediates diverse functions in bone marrow-derived phagocytes, including phagocytosis and microbe destruction. This cytokine has also been detected at implantation sites under both physiological pathological conditions many different species. At these particular sites, the outermost embryonic cell layer close contact with maternal tissues, trophoblast exhibits intense phagocytic activity. To determine whether IFN-gamma affects of mouse-trophoblast cells,...

Cytochemical localization of hydrogen peroxide-generating sites suggests NADPH (nicotinamide adenine dinucleotide 3-phosphate [reduced form]) oxidase expression at the maternal-fetal interface. To explore this possibility, we have characterized and activity complex in trophoblast cells during postimplantation period. Implantation ectoplacental cones (EPCs) from 7.5-gestational day embryos CD1 mice were used as a source for analyses catalytic regulatory subunits. EPCs grown primary culture to...

Preeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and edema, is major cause of fetal maternal morbidity mortality especially in developing countries. Bj-PRO-10c, proline-rich peptide isolated from Bothrops jararaca venom, has been attributed with potent anti-hypertensive effects. Recently, we have shown that Bj-PRO-10c-induced actions involved NO production spontaneous hypertensive rats. Using vitro studies now show Bj-PRO-10c was able to increase human...

Fetal gastroschisis is a paraumbilical abdominal wall defect with herniation of organs. The underlying cause the disease remains unknown; however, studies suggest that nutritional factors may play role in its development. This prospective case‐control study explored association serum fatty acid levels pregnant women and occurrence gastroschisis. Gastroschisis group comprised 57 fetuses gastroschisis, control 114 normal fetuses. Serum acids were compared between groups for overall pregnancy...

Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83 Problem In this study, we explored the relationship between cells (DC) and (IFN)‐γ, presence or absence ectoplacental (EC) using a coculture system. Method study Decidual EC were isolated from pregnant mice on gestation day 7.5. DCs cultured...

To assess the expression of decidual natural killer (dNK) cells and their cytokines in twin pregnancies with preeclampsia.This was a prospective case-control study. The inclusion criteria were diamniotic (monochorionic or dichorionic) third trimester negative serological results for infectious diseases; absence major fetal abnormalities twin-twin transfusion syndrome; no history administration corticosteroids this pregnancy. control group (CG) included uncomplicated pregnancies, preeclampsia...

We have previously shown that trophoblast can generate nitric oxide (NO) and express inducible isoform of synthase (iNOS). Moreover, this production was changed by the presence interferon-gamma (IFN-gamma) establishing a relationship between inductive response proinflammatory cytokine.As intracellular signal transduction pathway used IFN-gamma in target cells is Janus kinase (JAK)-signal transducer transcription activator (STAT), here we analyzed mouse effect staurosporine on mRNA protein...

Problem We hypothesized that trophoblast expression of Ccl25 attracts a specific leukocyte cell population to the implantation site for local regulation. Method study Mice blastocysts, ectoplacental cones, and decidua at gestational days 3.5‐7.5 were evaluated Ccr9 expressions. Peripheral availability characterization Ccr9+ leukocytes determined by flow cytometry. Leukocyte chemotaxis was assessed in presence recombinant protein embryos using antisense oligomers (ODNs) neutralizing antibody....

Abstract Objectives To investigate the association between selected single nucleotide polymorphisms (SNPs) with cervical insufficiency and its relationship obstetric history. Methods Twenty-eight women (case group) 29 non-pregnant (control were included. The SNPs sequenced included rs2586490 in collagen type I alpha 1 chain ( COL1A1 ), rs1882435 IV 3 COL4A3 rs2277698 metallopeptidase inhibitor 2 TIMP2 rs1800468 transforming growth factor beta TGFB1 ). Results We found a higher frequency of...

To evaluate the performance of adiponectin in predicting insulin need during pregnancy. Case-control analysis, nested from an ongoing prospective cohort, including 254 singleton pregnancies women with gestational diabetes mellitus (GDM) at a tertiary teaching hospital. Patients were divided into 2 groups (DIET group and INSULIN group) compared regarding clinical laboratory variables. After univariate variables that presented statistical significance or relevance included logistic regression....

The pattern of expression a variety placental nitric oxide synthase isoforms has contributed to elucidating the regulatory mechanisms (NO) synthesis during gestation. maintenance vascular tone, attenuation vasoconstriction, prevention platelet and leukocyte adhesion trophoblast surface, possible participation in uterine blood flow seem be main functions NO generated at fetal-maternal interface humans mice. Extending this knowledge other rodent species commonly used as laboratory animals,...