A5001823392- Nerve injury and regeneration
- Traumatic Brain Injury and Neurovascular Disturbances
- Traumatic Brain Injury Research
- S100 Proteins and Annexins
- Signaling Pathways in Disease
- Traumatic Ocular and Foreign Body Injuries
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Anesthesia and Neurotoxicity Research
- Aldose Reductase and Taurine
- Axon Guidance and Neuronal Signaling
- Disaster Response and Management
- Hereditary Neurological Disorders
- Tendon Structure and Treatment
- RNA Interference and Gene Delivery
- Complement system in diseases
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Redox biology and oxidative stress
- Calpain Protease Function and Regulation
- Trauma and Emergency Care Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Neuroscience and Neuropharmacology Research
- Cancer-related gene regulation
Karolinska Institutet
2004-2015
Mild traumatic brain injury (mTBI) is one of the most common neuronal insults and can lead to long-term disabilities. mTBI occurs when head exposed a rapid acceleration-deceleration movement triggering axonal injuries. Our limited understanding underlying pathological changes makes it difficult predict outcome mTBI. In this study we used scalable rat model for rotational acceleration TBI, previously characterized threshold pathology. We have analyzed whether TBI just above defined would...
Time-dependent changes in blood-based protein biomarkers can help identify the -pathological processes blast-induced traumatic brain injury (bTBI), assess severity, and monitor disease progression. We obtained blood from control injured mice (exposed to a single, low-intensity blast) at 2-h, 1-day, 1-week, 1-month post-injury. then determined serum levels of related metabolism (4-HNE, HIF-1α, ceruloplasmin), vascular function (AQP1, AQP4, VEGF, vWF, Flk-1), inflammation (OPN, CINC1,...
The Schwann cell basal lamina (BL) is required for normal myelination. Loss or mutations of BL constituents, such as laminin-2 (α2β1γ1), lead to severe neuropathic diseases affecting peripheral nerves. function the second known laminin present in BL, laminin-8 (α4β1γ1), so far unknown. Here we show that absence α4 chain, which distinguishes from laminin-2, leads a disturbance radial sorting, impaired myelination, and signs ataxia proprioceptive disturbances, whereas axonal regenerative...
Injured neurons become dependent on trophic factors for survival. However, application of to the site injury is technically extremely challenging. Novel approaches are needed circumvent this problem. Here, we unravel mechanism emergence dependency injured brain-derived neurotrophic factor (BDNF) Based mechanism, propose use diuretic bumetanide prevent requirement BDNF and consequent neuronal death in areas. Responses neurotransmitter GABA change from hyperpolarizing intact depolarizing...
A penetrating traumatic brain injury (pTBI) occurs when an object impacts the head with sufficient force to penetrate skin, skull, and meninges, inflict directly parenchyma. This type of has been notoriously difficult model in small laboratory animals such as rats or mice. To this end, we have established a novel non-fatal for pTBI based on modified air rifle that accelerates pellet, which turn probe then causes experimental animal's brain. In present study, focused acute phase characterized...
Abstract The favorable prognosis of regeneration in the peripheral nervous system after axonal lesions is generally regarded as dependent on Schwann cell basal lamina. Laminins, a heterotrimeric group lamina molecules, have been suggested to be among factors playing this supportive role. For neurons utilize laminin substrate for growth, an expression binding receptors, integrins, necessary. In study, we examined integrin subunits dorsal root ganglion (DRG) transection either their...
Major histocompatibility complex (MHC) class I molecules have fundamental functions in the immune system. Recent studies suggested that these may also non-immune nervous system, particular related to synaptic function and plasticity. Because adult motoneurons express mRNAs for MHC molecules, we examined their subcellular expression pattern vivo role connectivity of neurons. We observed immunoreactivity classical (Ia) protein motoneuron somata, but predominant was found axons presynaptically...
Brain trauma induces a multitude of reactions at molecular, cellular, and tissue levels, some which are beneficial to recovery, whereas others detrimental. Osteopontin (OPN), glycosylated phosphoprotein, can be found in both the soluble form as an extracellular matrix constituent several tissues vertebrate body, but its function after brain injury is largely unknown. In this study, expression OPN experimental traumatic rats was examined effects on hippocampal neurons cortical astrocytes were...
Penetrating traumatic brain injury (pTBI) has been difficult to model in small laboratory animals, such as rats or mice. Previously, we have established a non-fatal, rat for pTBI using modified air-rifle that accelerates pellet, which hits probe then penetrates the experimental animal's brain. Knockout and transgenic strains of mice offer attractive tools study biological reactions induced by TBI. Hence, present study, adapted our be used with The technical characterization impact device...
Replantation of avulsed spinal ventral roots has been show to enable significant and useful regrowth motor axons in both experimental animals human clinical cases, making up an interesting exception the rule unsuccessful neuronal regeneration central nervous system. Compared avulsion without repair, root replantation seems rescue lesioned motoneurons from death. In this study we have analyzed acute response adult rats with gene arrays combined cluster analysis ontology search terms. The data...
Integrins are cell surface receptors known to be important for regeneration in the peripheral nervous system. We have investigated expression of integrin messenger RNAs red nucleus neurons adult rats after axotomy and administration neurotrophic factors. Using radioactive situ hybridization, RNA subunits beta1, alpha3, alpha7 alphaV could detected. No change any alpha subunit detected axotomy. In contrast, a small upregulation beta1 was lesion. Administration neurotrophin-3 induced robust...
Abstract Grafting fetal ventral mesencephalon has been utilized to alleviate the symptoms of Parkinson's disease. One obstacle in using this approach is limited outgrowth from transplanted dopamine neurons. Thus, it important evaluate factors that promote Proteoglycans (PGs) are extracellular matrix molecules modulate neuritic growth. This study was performed role PGs nerve fiber formation organotypic slice cultures mesencephalon. Cultures were treated with PG synthesis inhibitor...
A penetrating traumatic brain injury (pTBI) occurs when an object impacts the head with sufficient energy to penetrate skin, cranial bone and meninges inflict directly parenchyma. This type of is particularly common in areas plagued by armed conflicts or gun-related violence. However, other causes pTBI exist such as violent stabbings, various types accidents even animal attacks. often associated intracranial hemorrhage, edema, ischemia a high risk infection. Therefore, classified severe form...