A5040797098- Aquaculture disease management and microbiota
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Vibrio bacteria research studies
- Aquaculture Nutrition and Growth
- Invertebrate Immune Response Mechanisms
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Oral microbiology and periodontitis research
- Viral Infections and Vectors
- Complement system in diseases
- Parasite Biology and Host Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- vaccines and immunoinformatics approaches
- RNA Interference and Gene Delivery
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Galectins and Cancer Biology
- Pneumocystis jirovecii pneumonia detection and treatment
- Radiomics and Machine Learning in Medical Imaging
- Genetic factors in colorectal cancer
- Parathyroid Disorders and Treatments
- Vector-borne infectious diseases
- Physiological and biochemical adaptations
- Leptospirosis research and findings
Catalyst Biosciences (United States)
2024
Ascendis Pharma (Denmark)
2022
Community Catalyst
2022
University of Copenhagen
2006-2013
Technical University of Denmark
2012
Statens Serum Institut
2004
Danish Cancer Society
2004
ABSTRACT Recombinant, immunodominant antigens derived from Mycobacterium tuberculosis can be used to effectively vaccinate against subsequent infection. However, the efficacy of these recombinant proteins is dependent on adjuvant for their delivery. This problem affects many potential vaccines, not just those tuberculosis, so discovery adjuvants that promote development cell-mediated immunity great interest. We have previously shown combination cationic surfactant dimethyl dioctadecyl...
A key hallmark of the vertebrate adaptive immune system is generation antigen-specific antibodies from B cells. Fish are most primitive gnathostomes (jawed vertebrates) possessing an system. Vaccination rainbow trout against enteric redmouth disease (ERM) by immersion in Yersinia ruckeri bacterin confers a high degree protection to fish. The mechanisms responsible for may comprise both cellular and humoral elements but role specific immunoglobulins this has been questioned not previously...
AbstractThe identification of tumor antigens, which are essential for the survival cells is a new avenue to prevent antigen loss variants emerging due immunoselection, particularly during immune therapy. In search such immunogenic we recently identified spontaneous cytotoxic lymphocyte (CTL) responses against inhibitor apoptosis protein survivin. Thus, two HLA-A2-restricted, survivin-derived CTL epitopes, both were targets in melanoma, breast cancer, and CLL. Here, extend these data describe...
Abstract Background Intratumoral (IT) delivery of toll-like receptor (TLR) agonists has shown encouraging anti-tumor benefit in preclinical and early clinical studies. However, IT TLR may lead to rapid effusion from the tumor microenvironment (TME), potentially limiting duration local inflammation increasing risk systemic adverse events. Methods To address these limitations, TransCon ™ TLR7/8 Agonist—an investigational sustained-release prodrug resiquimod that uses a linker hydrogel...
Furunculosis caused by infection with Aeromonas salmonicida subsp. has been a known threat to aquaculture for more than century. Efficient prophylactic approaches against this disease are essential continued growth of salmonid aquaculture. Since the introduction successful oil-adjuvanted vaccines in early 1990's, number studies have published on protective as well adverse effects these vaccines. Most focus vaccination salmon (Salmo salar). However, rainbow trout (Oncorhynchus mykiss) also...
ABSTRACT Hypoparathyroidism (HP) is a condition of parathyroid hormone (PTH) deficiency leading to abnormal calcium and phosphate metabolism. The mainstay therapy consists vitamin D supplements, as well adjunct Natpara (PTH(1-84)). However, neither optimally controls urinary (uCa) or significantly reduces the incidence hypercalcemia hypocalcemia. TransCon PTH, sustained-release prodrug PTH(1-34) in development for treatment HP, was designed overcome these limitations. To determine...
Rainbow trout (Oncorhynchus mykiss) were immunized with plasmid DNA vaccine constructs encoding selected antigens from the parasite Ichthyophthirius multifiliis. Two immobilization (I-ags) and one cysteine protease tested as genetic antigen candidates. Antigenicity was evaluated by immunostaining of transfected fish cells using I-ag specific mono- polyclonal antibodies. I. multifiliis antibody production, regulation immune-relevant genes and/or protection in terms burden or mortality...
Abstract Head kidney leucocytes are central elements in a number of vivo and vitro assays elucidating innate adaptive immune mechanisms teleosts following stimulation with various antigens. These systems sensitive to several factors affecting the outcome assays. The present work describes importance temperature, cell concentration, exposure time immune-modulatory molecules on respiratory burst activity (RBA) rainbow trout head vitro. Some variation RBA was observed among individual fish....
Background Despite vaccination with a commercial vaccine documented protective effect against Vibrio anguillarum O1 disease outbreaks caused by this bacterium have been registered among rainbow trout at Danish fish farms. The present study examined specific serum antibody levels as valid marker for assessing status in population. For purpose highly sensitive enzyme-linked immunosorbent assay (ELISA) was developed and used to evaluate sera from farmed vaccinated V. O1. Study Design Immune...
Background Furunculosis, caused by Aeromonas salmonicida, continues to be a major health problem for the growing salmonid aquaculture. Despite effective vaccination programs regular outbreaks occur at fish farms calling repeated antibiotic treatment. We hypothesized that difference in natural susceptibility this disease might exist between Baltic salmon and widely used rainbow trout. Study Design A cohabitation challenge model was applied investigate relative infection with A. salmonicida...
Background and aims. A prospective cross-sectional study was designed to test if total levels of TIMP-1 in saliva plasma correlated with the diagnosis colorectal cancer (CRC) a population symptoms consistent this disease. Materials methods. Stimulated whole blood samples were collected from 161 individuals referred colonoscopy associated CRC. The results examination, as well previous and/or current other diseases recorded. In blinded study, authors used an in-house ELISA previously validated...
Salivary tissue inhibitor of metalloproteinases-1 (TIMP-1) has been associated with pathological conditions in the oral cavity, but origin TIMP-1 saliva remains unknown. Hence, we studied localization salivary gland and also investigated if found blood is identical. Human samples (four parotid four submandibular biopsies) were analysed for presence mRNA protein expression. To assess glycosylation profiles saliva, was isolated from plasma unstimulated stimulated whole as well by MALDI-TOF...
Objective. Tissue inhibitor of metalloproteinases 1 (TIMP‐1) has been identified as a potential biomarker in diseases such cancer, cardiovascular and diabetes. Since TIMP‐1 resides most tissues bodily fluids, we evaluated the using saliva to obtain reproducible measurements non‐invasive manner. Material methods. Samples unstimulated stimulated whole collected from individual glands were analysed for content. A ELISA was validated use testing optimal sampling handling procedures identified....
Furunculosis caused by infection with Aeromonas salmonicida subsp.salmonicida has been a known threat to aquaculture for more than century.Efficient prophylactic approaches against this disease are essential continued growth of salmonid aquaculture.Since the introduction successful oil-adjuvanted vaccines in early 1990's, number studies have published on protective as well adverse effects these vaccines.Most focus vaccination salmon (Salmo salar).However, rainbow trout (Oncorhynchus mykiss)...
Interspecies scaling of the pharmacokinetics (PK) CB 4332, a 150 kDa recombinant complement factor I protein, was performed using traditional and model-based approaches to inform first-in-human dose selection. Plasma concentration versus time data from four preclinical PK studies single intravenous subcutaneous (SC) 4332 dosing in mice, rats nonhuman primates (NHPs) were modeled simultaneously naive pooling including allometric scaling. The human-equivalent calculated no observed adverse...
Abstract Background : The complement system plays a central role in innate immune response with the recognition and elimination of pathogens through direct killing and/or stimulation phagocytosis. is composed complex proteolytic cascade more than 50 soluble membranous proteins among which Factor I (FI) key protease that regulates inactivation C3b C4b their downstream effect terminal membrane attack (MAC) formation. A deficiency or absence endogenous FI results uncontrolled spontaneous...