The high demand of fresh-like products to meet the fruits and vegetables serving encouraged implementation non-thermal food processing techniques, such as pressure (HPP) pulsed electric fields (PEF), with low impact on nutritional components. aim present work is evaluate application HPP PEF techniques useful decontamination tool for aflatoxins (AFs) reduction in grape juice. Spiked juice samples AFs treated by or were extracted using dispersive liquid-liquid microextraction (DLLME)...

Mycotoxins are produced by a number of fungal genera spp., for example, Aspergillus, Penicillium, Alternaria, Fusarium, and Claviceps. Beauvericin (BEA) Ochratoxin A (OTA) present in various cereal crops processed grains. This goal this study was to determine their combination effect HepG2 cells, presented the first time. In study, type interaction among BEA OTA through an isobologram method, cell cycle disturbance flow cytometry, genotoxic potential vitro micronucleus (MN) assay following...

A new analytical method for the simultaneous determination of enniatins (ENs) and beauvericin (BEA) in fish feed tissues by liquid chromatography coupled to mass spectrometry with linear ion trap (LC-MS/MS-LIT) was developed. Results showed that developed is precise sensitive. The presence emerging Fusarium mycotoxins, ENs BEA, determined samples aquaculture farmed fish, showing all analyzed were contaminated 100% coexistence. In aquacultured samples, highest incidence found edible muscle...

Many toxigenic fungi are devastating pathogens of crop, fruit, and vegetable diseases worldwide [...]

A simple and rapid multi‐mycotoxin method for the determination of 17 mycotoxins simultaneously is described in present survey on durum soft wheat pasta samples. Mycotoxins included study were those mainly reported cereal samples: ochratoxin‐A (OTA), aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON), 3‐and 15‐acetyl‐deoxynivalenol (3‐AcDON 15‐AcDON), nivalenol (NIV), neosolaniol (NEO), fusarenon‐X, (FUS‐X), T‐2 toxin (T‐2) HT‐2 (HT‐2), fumonisin B2 (FB1 FB2), four emerging...

A comprehensive strategy combining a quantitative method for 28 mycotoxins and post-target screening other 245 fungal bacterial metabolites in dry pet food samples were developed using an acetonitrile-based extraction ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method. The proposed showed satisfactory validation results according Commission Decision 2002/657/EC. Average recoveries from 72 108% obtained all studied...

Emerging fusariotoxins, mainly enniatins (ENNs) and beauvericin (BEA), are secondary toxic metabolites produced by Fusarium spp. widely distributed contaminants of cereals by-products. Mycotoxin contamination in these products supposes an important risk to feed supply security the industry due common use formulations. Hence, continuous monitoring both raw materials mixtures is highly recommended as stated sanitary authorities. Therefore, analytical procedure based on liquid chromatography...

A 28-day repeated dose preliminary assay, using enniatin naturally contaminated feed through microbial fermentation by a Fusarium tricinctum strain, was carried out employing 2-month-old female Wistar rats as in vivo experimental model. In order to simulate physiological test of toxic compound produced fungi, five treated animals were fed during 28 days with fermented feed. As control group, standard At the 28th day, blood samples collected for biochemical analysis and gastrointestinal...

The Varroa destructor parasite is responsible for varroasis in honeybees worldwide, the most destructive disease among parasitic diseases. Thus, different insecticides/acaricides have been widely used within beehives to control these Namely, amitraz acaricide due its high efficacy shown against destructor. However, pesticides beehive treatments could be incorporated into honey and accumulate other hive products. Hence, honeybee health impairment of quality caused by gained more attention....

The inclusion of vegetal raw materials in feed for fish farming has increased the risk mycotoxin occurrence feed, as well edible tissues from fed with contaminated due to carry-over muscle portions. Therefore, objective this study was evaluate 15 mycotoxins processed products, which are commonly consumed, such smoked salmon and trout, different types sushi, gula substitutes. A QuEChERS method employed perform extraction samples. For identification quantitation, selected technique liquid...

The development of innovative food processing technologies has increased to answer the growing demand supply fresh-like products. aim present study is investigate effect pulsed electric fields (PEF) technology on reducing emerging mycotoxins (enniatins (ENs) and beauvericin (BEA)) contents in juice smoothie samples. products degradation obtained after PEF treatment were identified their toxicological endpoint toxicities predicted by Pro Tox-II web. Mycotoxin reduction ranged from 43 70%...

The present study aimed to identify mycotoxins in edible tissues of Atlantic salmon (Salmo salar) using liquid chromatography coupled hybrid quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). After a non-targeted screening approach and home-made spectral library, 233 were analyzed. Moreover, the occurrence fish filets was evaluated, their potential toxicity predicted by silico methods. According obtained results, forty identified analyzed samples, predominant being enniatins (also...

Mycotoxins are secondary metabolites produced by certain filamentous fungi. They common contaminants found in a wide variety of food matrices, thus representing threat to public health, as they can be carcinogenic, mutagenic, or teratogenic, among other toxic effects. Several hundreds mycotoxins have been reported, but only few them regulated, due the lack data regarding their toxicity and mechanisms action. Thus, more comprehensive evaluation foodstuffs is required. In silico toxicology...

The aim of the present work was to study occurrence mycotoxins [aflatoxins (1–4), 3-acetyldeoxyniavlenol (5), 15-acetyldeoxynivalenol (6), nivalenol (7), HT-2 (8), T-2 (9), ochratoxin A (10), zearalenone (11), enniatin (12), A1 (13), B (14), B1 (15), and beauvericin (16)] in potable products derived from herbal teas. Analysis carried out by liquid chromatography coupled ion-trap tandem mass spectrometry (LC-MS/MS-IT) after a dispersive liquid–liquid microextraction procedure (DLLME)...

Celiac disease (CD) is a genetic-based autoimmune disorder which characterized by inflammation in the small intestinal mucosa due to intolerance gluten. people should consume products without gluten, are elaborated mainly with maize or other cereals. Contamination of cereals mycotoxins, such as fumonisins (FBs) and aflatoxins (AFs) frequently reported worldwide. Therefore, food ingestion main source mycotoxin exposure. A new analytical method was developed validated for simultaneous analysis...