A5003879604- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- RNA Research and Splicing
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Redox biology and oxidative stress
- Plant Micronutrient Interactions and Effects
- Lipid metabolism and biosynthesis
- Plant Virus Research Studies
- Silicon Effects in Agriculture
- Plant Reproductive Biology
- Ubiquitin and proteasome pathways
- Cell Image Analysis Techniques
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- Free Radicals and Antioxidants
- Genomics and Phylogenetic Studies
Valdosta State University
2022-2023
University of Tennessee at Knoxville
2015-2021
With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, stewardship, energy, technology healthcare. This effort requires conceptual technological framework identify map all cell types, comprehensively annotate localization organization molecules at cellular tissue levels. framework, called Plant Cell Atlas (PCA), for understanding...
During waterlogging and the associated oxygen deprivation stress, plants respond by induction of adaptive programs, including redirected expression gene networks toward synthesis core hypoxia-response proteins. Among these response proteins in Arabidopsis (Arabidopsis thaliana) is calcium sensor CML38, a protein related to regulator silencing calmodulin-like (rgsCaMs). CML38 transcripts are up-regulated more than 300-fold roots within 6 h hypoxia treatment. Transfer DNA insertional mutants...
Cytosolic mRNA translation is subject to global and mRNA-specific controls. Phosphorylation of the initiation factor eIF2α anchors a reversible regulatory switch that represses cytosolic globally. The stress-responsive GCN2 kinase only known for serine 56 in Arabidopsis (Arabidopsis thaliana). Here, we show conditions generate reactive oxygen species (ROS) chloroplast, including dark-light transitions, high light, herbicide methyl viologen, rapidly activated kinase, whereas mitochondrial...
Regulation of cytosolic mRNA translation is a key node for rapid adaptation to environmental stress conditions. In yeast and animals, phosphorylation the -subunit eukaryotic initiation factor eIF2 most thoroughly characterized event in regulating global under stress. plants, GCN2 kinase (General Control Non-derepressible-2), only known eIF2. activated variety stresses including reactive oxygen species. Here we provide new evidence that Arabidopsis also rapidly light dependent manner by cold...
Under anaerobic stress, Arabidopsis thaliana induces the expression of a collection core hypoxia genes that encode proteins for an adaptive response. Among these is NIP2;1, which encodes member "Nodulin 26-like Intrinsic Protein" (NIP) subgroup aquaporin superfamily membrane channel proteins. NIP2;1 limited to "anoxia core" region root stele under normal growth conditions, but shows substantial induction (up 1,000-fold by 2-4 h hypoxia) low oxygen and accumulation in all tissues. During...
The ErbB-3 BINDING PROTEIN 1 (EBP1) drives growth, but the mechanism of how it acts in plants is little understood. Here, we show that EBP1 expression and protein abundance Arabidopsis (Arabidopsis thaliana) are predominantly confined to meristematic cells induced by sucrose partially dependent on TARGET OF RAPAMYCIN (TOR) kinase activity. Consistent with being downstream TOR, silencing restrains, while overexpression promotes, root mostly under sucrose-limiting conditions. Inducible...
The protein kinase GCN2 (General Control Nonderepressible2) and its phosphorylation target, the eukaryotic translation initiation factor (eIF)2α represent core module of plant's integrated stress response, a signaling pathway widely conserved in eukaryotes that can rapidly regulate response to stressful conditions. Recent findings indicate Arabidopsis thaliana operates under command reactive oxygen species (ROS) emanating from chloroplast variety abiotic stresses such as excess light. To get...
Polysome profiling by sucrose density gradient centrifugation is commonly used to study the overall degree of translation (messenger RNA protein synthesis). Traditionally, method begins with synthesis a 5-10 mL onto which 0.5-1 cell extract layered and centrifuged at high speed for 3-4 h in floor-model ultracentrifuge. After centrifugation, solution passed through an absorbance recorder generate polysome profile. Ten twelve fractions (0.8-1 each) are collected isolating different...
ABSTRACT Cytosolic mRNA translation is subject to global and mRNA-specific controls. Phosphorylation of initiation factor eIF2α anchors a reversible switch that represses globally. The stress-responsive GCN2 kinase the only known for in Arabidopsis . Here we show conditions generate reactive oxygen species (ROS) chloroplast, such as dark-light transitions, high light, herbicide methyl viologen all rapidly activated kinase, whereas mitochondrial ER stress did not. In addition, activation was...
Abstract Regulator-of-gene-silencing calmodulins (rgsCaM) represent a phylogenetic subfamily of calmodulin-like calcium sensors that are targets viral induced suppression posttranscriptional gene silencing by secondary siRNAs. The present work shows stress (hypoxia) induces mRNP granule formation also the relocalization rgsCaM to cytosolic granule-like foci interact with surface and processing body structures. Co-expression its binding protein Suppressor Gene Silencing 3 causes...
ABSTRACT Regulation of cytosolic mRNA translation is a key node for rapid adaptation to environmental stress conditions. In yeast and animals, phosphorylation the α-subunit eukaryotic initiation factor eIF2 most thoroughly characterized event in regulating global under stress. plants, GCN2 kinase ( General Control Non-derepressible-2 ) only known eIF2α. activated variety stresses including reactive oxygen species. Here we provide new evidence that Arabidopsis also rapidly light dependent...
Understanding silique and seed morphology is essential to developmental biology. Arabidopsis thaliana one of the best-studied plant models for understanding genetic determinants count size. However, small size its seeds, their encasement in a pod known as silique, makes investigating numbers both time consuming tedious. Researchers often report bulk weights an indicator average size, but this overlooks individual details. Removal seeds subsequent image analysis possible, automated counts are...
Abstract Under anaerobic stress Arabidopsis thaliana induces the expression of a collection core hypoxia genes that encode proteins associated with an adaptive response. Included in these is NIP2;1 , which encodes member “Nodulin-like Intrinsic Protein” (NIP) subgroup aquaporin superfamily membrane channel proteins. normal growth, limited to “anoxia core” region root stele, but shows substantial induction response low oxygen (as high as 1000-fold by 2-4 hr challenge), and accumulates all...