A5102780098- RNA Research and Splicing
- Cancer-related gene regulation
- Bone fractures and treatments
- Genomics and Chromatin Dynamics
- HER2/EGFR in Cancer Research
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Diversity and Career in Medicine
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Global Health Workforce Issues
- Orthopedic Surgery and Rehabilitation
- Total Knee Arthroplasty Outcomes
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Protein Degradation and Inhibitors
- Shoulder Injury and Treatment
- Hip and Femur Fractures
- Geriatric Care and Nursing Homes
- NF-κB Signaling Pathways
- Birth, Development, and Health
- Helicobacter pylori-related gastroenterology studies
National Institutes of Health
1998-2021
Office of Management
2021
Auburn University
2021
Office of the Director
2021
Center for Cancer Research
2003-2010
National Cancer Institute
1988-2010
Guy's and St Thomas' NHS Foundation Trust
2007
St Thomas' Hospital
2003-2007
University of Illinois Urbana-Champaign
2005
Beth Israel Deaconess Medical Center
2001
Assisted living (AL) has existed in the United States for decades, evolving response to older adults' need supportive care and distaste nursing homes models of congregate care. AL is state-regulated, provides at least 2 meals a day, around-the-clock supervision, help with personal care, but not licensed as home.The key constructs originally conceived were provide person-centered promote quality life through responsive services meet scheduled unscheduled needs assistance, an operating...
The epidermal growth factor receptor (EGFR) is expressed at different levels in many cell types and found overexpressed cancers. EGFR expression increased or decreased response to extracellular stimuli. We examined the effect of c-Jun on promoter activity. used DNAse I foot-printing analysis determine binding activator protein 1 (AP-1) region. also cotransfection experiments western blotting AP-1 family members expression. was able bind least seven sites Cotransfection MCF-7 cells with a...
Tumor suppressor p53 has been shown to transactivate epidermal growth factor receptor (EGFR) expression through binding a putative responsive element in the EGFR promoter between nucleotides −265 and −239 (EGFRp53RE). Isotypes of p63 gene products, recently identified as relatives, have similar function several target promoters. However, our results indicate that TAp63γ very low ability bind EGFRp53RE surprisingly represses both basal activity endogenous expression. Transient transfection...
Abstract —Platelet-derived growth factor (PDGF) is a mitogen and chemoattractant for wide variety of cell types. The genes encoding PDGF A chain (PDGF-A) B (PDGF-B) reside on separate chromosomes are independently regulated at the level transcription. Regulatory events underlying inducible PDGF-A expression have been focus much investigation. However, mechanisms that inhibit transcription this gene not well understood. In study, we report capacity newly cloned DNA binding factor, GC 2...
The response of the epidermal growth factor (EGF) receptor gene to phorbol 12-myristate 13-acetate (PMA) was analyzed using nuclei and nuclear extracts prepared from PMA-treated KB cells. Transient transfection assays run-off experiments showed that PMA increased EGF transcription. Cell-free transcription with promoter mutants revealed region containing nucleotides −150 −16 sufficient for inducibility. A fragment −167 −105 binding a present in When this partially purified by column...
Helicobacter pylori infection of the gastric mucosa is strongly associated with gastritis, peptic ulcer disease, and cancer. However, mechanisms by which H. causes cancer are currently unknown. Binding epidermal growth factor (EGF) to its receptor (EGFR) may be important in development This interaction accelerates cell proliferation migration, triggers epithelial signaling. In this study, we investigated effects on EGFR- AP-1-mediated signal transduction pathways AGS line tissue from...
Our aim was to explore the involvement of transcriptional suppressor GCF2 in silencing RhoA, disorganization cytoskeleton, mislocalization MRP1, and sensitivity anticancer agents as an upstream gene target cancer therapy. Increased expression found human cisplatin-resistant cells, overexpression GCF2-transfected cells results loss RhoA disruption actin/filamin network. In consequence, membrane transporter MRP1 internalized from cell surface into cytoplasm, rendering sensitive doxorubicin by...
Recently, we characterized the rat epidermal growth factor receptor (EGFR) promoter and demonstrated that TCC repeat sequences are required for down-regulation of EGFR by nerve (NGF) in PC12 cells. In this study, report Wilms' tumor gene product WT1, a zinc finger transcription factor, is able to enhance activity cotransfection assays. Gel mobility shift assays demonstrate WT1 binds promoter. Overexpression resulted up-regulation expression levels endogenous Interestingly, NGF down-regulated...
The cell autonomous response to viral infection is carefully regulated induce type I interferons (IFNs), which in turn the establishment of an antiviral state. Leucine-rich repeat (in Flightless I) interacting protein-1 (LRRFIP1) and LRRFIP2 are 2 related proteins that have been identified as with MyD88 homolog, a leucine-rich protein. positively regulates NFκB macrophage cytokine production after lipopolysaccharide, but less known about LRRFIP1. We hypothesized LRRFIP1 could be more...
GC-binding factor 2 (GCF2), a transcriptional repressor that decreases the activity of several genes is capable binding directly to GC-rich sequence EGFR promoter and repressing EGFR. In addition its function as repressor, GCF2 can interact with other proteins such flightless-1 (Fli-1). Many previous findings pertaining Fli-1 have suggested role for fli-1 in providing direct link between molecules involved signal transduction pathways actin cytoskeleton. We hypothesized GCF2, together Fli-1,...
GCF is a human transcriptional regulator that represses transcription of certain genes and encoded by 3-kilobase (kb) mRNA (Kageyama, R., Pastan, I. (1989) Cell 59, 815-825). The expression was examined in variety clonal cell lines. 3.0-kb found to be expressed at the highest level HUT 102 cells (derived from T-cell lymphoma). Elevated levels were also noted KATO III AGS (gastric carcinomas), FEM-X (melanoma), U266B1 (myeloma) A fibroblast line (WI38) did not express mRNA, no...
Treatment of PC12 cells with nerve growth factor leads to a decrease in the number epidermal receptors on cell membrane. The mRNA for receptor decreases comparable fashion. This appears due transcription gene because first, there is no difference stability mRNA, second, newly transcribed decreased factor-differentiated cells, and third, constructs containing promoter region are much less readily than untreated cells. not seen p140<sup>trk</sup>-deficient variant PC12nnr5 nor either...
Identification of the factors controlling transcription epidermal growth factor (EGF) receptor gene is essential for understanding regulation EGF and its overexpression in human carcinomas. In this study, we have identified a 60-base pair (bp) region (-919 to -860) relative AUG translation initiation codon 5' promoter that functions as cis-acting transcriptional repressor (ETR). This fragment also acted when linked thymidine kinase promoter. Gel mobility shift assays demonstrated...