Hassan Ashktorab
A5083409124- Genetic factors in colorectal cancer
- Colorectal Cancer Screening and Detection
- Helicobacter pylori-related gastroenterology studies
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Gastric Cancer Management and Outcomes
- MicroRNA in disease regulation
- Colorectal Cancer Treatments and Studies
- Cancer-related gene regulation
- Saffron Plant Research Studies
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- COVID-19 Clinical Research Studies
- Gut microbiota and health
- Long-Term Effects of COVID-19
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Digestive system and related health
- Hippo pathway signaling and YAP/TAZ
- Liver Disease Diagnosis and Treatment
- Circular RNAs in diseases
- Diverticular Disease and Complications
- Global Cancer Incidence and Screening
- Galectins and Cancer Biology
- Gastrointestinal disorders and treatments
- Medicinal Plants and Bioactive Compounds
Howard University
2016-2025
Howard University Hospital
2011-2024
Georgetown University
2024
Johns Hopkins Hospital
2024
New York University
2024
Massachusetts General Hospital
2024
Boston University
2024
Weatherford College
2020-2023
University of Pittsburgh Medical Center
2018-2020
Cancer Research Center
2013-2019
Abstract Purpose: Gut microbiota have been implicated in the development of colorectal cancer. We evaluated utility fecal bacterial marker candidates identified by our metagenome sequencing analysis for cancer diagnosis. Experimental Design: Subjects (total 439; 203 and 236 healthy subjects) from two independent Asian cohorts were included. Probe-based duplex quantitative PCR (qPCR) assays established quantification candidates. Results: Candidates sequencing, including Fusobacterium...
Despite multidisciplinary treatment for patients with advanced gastric cancer, their prognosis remains poor. Therefore, the development of novel therapeutic strategies is urgently needed, and immunotherapy utilizing anti‐programmed death 1/‐programmed ligand‐1 mA b an attractive approach. However, as there limited information on how programmed upregulated tumor cells within microenvironment, we examined mechanism regulation a particular focus interferon gamma in vitro setting clinical...
MicroRNA (miR) sponges containing miR binding sequences constitute a potentially powerful molecular therapeutic strategy. Recently, naturally occurring circular RNAs (circRNAs) were shown to function as efficient in cancer cells. We hypothesized that synthetic circRNA could achieve loss-of-function targeted against specific miRs. Linear RNA molecules miR-21 sites transcribed vitro; after dephosphorylation and phosphorylation, circularization was achieved using 5′-3′ end-ligation by T4 ligase...
Circular RNA (circRNA), a subclass of non-coding RNA, plays critical role in cancer tumorigenesis and metastasis. It has been suggested that circRNA acts as microRNA sponge or scaffold to interact with protein complexes; however, its full range functions remains elusive. Recently, some circRNAs have found coding potential.To investigate the gastric (GC), parallel sequencing was performed using five paired GC samples. Differentially expressed circAXIN1 proposed encode novel protein....
Importance A substantial number of individuals worldwide experience long COVID, or post-COVID condition. Other postviral and autoimmune conditions have a female predominance, but whether the same is true for especially within different subgroups, uncertain. Objective To evaluate sex differences in risk developing COVID among adults with SARS-CoV-2 infection. Design, Setting, Participants This cohort study used data from National Institutes Health (NIH) Researching to Enhance Recovery...
Abstract Background Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) may occur after infection. How often people develop ME/CFS SARS-CoV-2 infection is unknown. Objective To determine the incidence and prevalence of post-COVID-19 among adults enrolled in Researching COVID to Enhance Recovery (RECOVER-Adult) study. Design, Setting, Participants RECOVER-Adult a longitudinal observational cohort study conducted across U.S. We included participants who had visit at least 6 months no...
Background Mutations and promoters' methylation of a set candidate cancer genes (CAN genes) are associated with progression colorectal (CRC). We hypothesized that these genes' promoters inactivated through epigenetic silencing may show different profile in high-risk populations. investigated the status CAN gene CHD5 protein expression African American CRC tissue microarrays (TMA) using immunohistochemical staining. Methodology/Principal Findings The promoter was studied by...
Helicobacter pylori (H. pylori) is a common human pathogenic bacterium. Once infected, it difficult for the host to clear this organism using innate immune system. Increased antibiotic resistance further makes challenging effective eradication. However, mechanisms of evasion still remain obscure, and novel strategies should be developed efficiently eliminate H. infection in stomachs. Here we uncovered desirable anti-H. effect vitamin D3 both vitro vivo, even against antibiotic-resistant...
Abstract YAP and TAZ play oncogenic roles in various organs, but the role of YAP/TAZ gastric cancer remains unclear. Here, we show that activation initiates tumorigenesis vivo verify its significance human cancer. In mice, pyloric stem cell led to step-wise tumorigenesis. RNA sequencing identified MYC as a decisive target YAP, which controls at transcriptional posttranscriptional levels. These mechanisms tightly regulated homeostatic conditions, altered this balance by impeding miRNA...
Abstract According to recent studies, Cannabidiol (CBD), one of the main components Cannabis sativa , has anticancer effects in several cancers. However, exact mechanism CBD action is not currently understood. Here, promoted cell death gastric cancer. We suggest that induced apoptosis by suppressing X-linked inhibitor (XIAP), a member IAP protein family. reduced XIAP levels while increasing ubiquitination XIAP. The expression Smac, known XIAP, was found be elevated during treatment....
Colorectal cancer (CRC) is one of the most common cancers worldwide. One its subtypes associated with defective mismatch repair (dMMR) genes. Saffron has many potentially protective roles against colon malignancy. However, these in context dMMR tumors have not been explored. In this study, we aimed to investigate effects saffron and constituents CRC cell lines dMMR. crude extracts specific compounds (safranal crocin) were used human colorectal HCT116, HCT116+3 (inserted MLH1), HCT116+5...
Intestinal metaplasia (IM) is a pre-malignant condition of the gastric mucosa associated with increased cancer (GC) risk. Analyzing 1,256 samples (1,152 IMs) across 692 subjects from prospective 10-year study, we identify 26 IM driver genes in diverse pathways including chromatin regulation (ARID1A) and intestinal homeostasis (SOX9). Single-cell spatial profiles highlight changes tissue ecology lineage heterogeneity, an stem-cell dominant cellular compartment linked to early malignancy....
Importance SARS-CoV-2 infection can result in ongoing, relapsing, or new symptoms other health effects after the acute phase of infection; termed post-acute sequelae (PASC), long COVID. The characteristics, prevalence, trajectory and mechanisms PASC are ill-defined. objectives Researching COVID to Enhance Recovery (RECOVER) Multi-site Observational Study Adults (RECOVER-Adult) to: (1) characterize prevalence; (2) symptoms, organ dysfunction, natural history, distinct phenotypes PASC; (3)...
There are currently no validated clinical biomarkers of postacute sequelae SARS-CoV-2 infection (PASC).
Colon cancer (CRC) development often includes chromosomal instability (CIN) leading to amplifications and deletions of large DNA segments. Epidemiological, clinical, cytogenetic studies showed that there are considerable differences between CRC tumors from African Americans (AAs) Caucasian patients. In this study, we determined genomic copy number aberrations in sporadic AAs, order investigate possible explanations for the observed disparities.We applied genome-wide array comparative genome...
Background Chromodomain-helicase-DNA-binding protein 5 (CHD5) is a newly identified tumor suppressor that frequently downregulated in variety of human cancers. Our previous work revealed the low expression CHD5 colorectal cancer correlated with promoter CpG island hypermethylation. In this study, we investigated effect microRNA-211 (miR-211)-regulated on tumorigenesis. Methodology/Principal Findings miR-211 was predicted to target by TargetScan software analysis. A stably expressing...
Abstract Purpose: Lauren diffuse-type gastric adenocarcinomas (DGAs) are generally genomically stable. We identified lysine (K)-specific methyltransferase 2C (KMT2C) as a frequently mutated gene and examined its role in DGA progression. Experimental Design: performed whole exome sequencing on tumor samples of 27 patients with who underwent gastrectomy. Lysine was analyzed cell lines patient tumors. Results: KMT2C the most (11 tumors [41%]). expression by immunohistochemistry from 135...
// Kwang-Soo Lee 1,2,* , Jong-Lyul Park 3,4,* Kwanbok 1 Lauren E. Richardson 1,5 Betty H. Johnson Hyun-Sung 6 Ju-Seog Sang-Bae Kim Oh-Hyung Kwon 3 Kyu Sang Song 7 Yong Sung 3,4 Hassan Ashktorab 8 Duane T. Smoot Ho Jeon 2 Seon-Young and Sun 1. Department of Biochemistry Molecular Biology, The University Texas Medical Branch, Galveston, TX77555-1072, USA 2. Life Science Center for Aging Health Care, Hallym University, Chuncheon 200-702, Korea 3. Genomics Research Center, KRIBB, Daejeon...
The identification of genes that are differentially methylated in colorectal cancer (CRC) has potential value for both diagnostic and therapeutic interventions specifically high-risk populations such as African Americans (AAs). However, DNA methylation patterns CRC, especially AAs, have not been systematically explored remain poorly understood. Here, we performed methylome profiling to identify the status CpG islands within candidate involved critical pathways important initiation...