A5086358045- Liver physiology and pathology
- RNA modifications and cancer
- Cancer-related gene regulation
- Organ Transplantation Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Liver Disease Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Lung Cancer Treatments and Mutations
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- biodegradable polymer synthesis and properties
- Cancer-related molecular mechanisms research
- Hydrogels: synthesis, properties, applications
- Lung Cancer Research Studies
- RNA and protein synthesis mechanisms
- Lung Cancer Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- 14-3-3 protein interactions
- S100 Proteins and Annexins
- Macrophage Migration Inhibitory Factor
- Uterine Myomas and Treatments
- TGF-β signaling in diseases
University of Tsukuba
2011-2022
Hillel Yaffe Medical Center
2011
Ghent University
2011
INCLIVA Health Research Institute
2011
Center of Regenerative Medicine in Barcelona
2011
Universidade Estadual de Campinas (UNICAMP)
2011
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2006-2007
Institute of Physiology and Basic Medicine
2006
National Institute of Advanced Industrial Science and Technology
1997
National Institute for Interdisciplinary Science and Technology
1996
Endometrial stromal sarcoma (ESS) of the uterus is a rare uterine malignancy that has not been characterized in detail. To characterize phenotype ESS uterus, we extracted RNA from and stroma normal endometrium using tissue microdissection system compared expression profiles two tissues. After suppression subtractive hybridization differential screening, detected metastasis‐associated lung adenocarcinoma transcript 1 ( MALAT‐1 ) gene as one major genes upregulated ESS, full‐length placental...
Recent genetic analysis has identified frequent mutations in ten-eleven translocation 2 (TET2), DNA methyltransferase 3A (DNMT3A), isocitrate dehydrogenase (IDH2) and ras homolog family member A (RHOA) nodal T-cell lymphomas, including angioimmunoblastic lymphoma peripheral lymphoma, not otherwise specified. We examined the distribution of these subtypes mature T-/natural killer cell neoplasms to determine their clonal architecture. Targeted sequencing was performed for 71 genes...
Adenocarcinoma in situ (AIS) of the lung has an extremely favorable prognosis, with a 5-year survival rate 100%. However, early invasive adenocarcinoma (EIA) often fatal outcome. In this study, we compared expression profiles AIS those EIA showing lymph node metastasis or outcome, and screened differentially expressed genes by cDNA microarray. From selected, focused on Stratifin (SFN, 14-3-3 σ), which showed significantly higher than AIS. Immunohistochemistry for SFN revealed that more 95%...
Adenocarcinoma in situ (AIS) of the lung has an extremely favorable prognosis. However, early but invasive adenocarcinoma (eIA) sometimes a fatal outcome. We had previously compared expression profiles AIS with those eIA showing lymph node metastasis or outcome, and found that stratifin (SFN, 14-3-3 sigma) was differentially expressed gene related to cell proliferation. Here, we performed vivo study clarify role SFN initiation progression adenocarcinoma. Suppression A549 (a human line) by...
Cellular mechanical properties are potential cancer biomarkers used for objective cytology to replace the current subjective method relying on cytomorphology. However, heterogeneity among intra/intercellular mechanics and interplay between cytoskeletal prestress elastic modulus obscured difference detectable malignant benign cells. In this work, we collected high density nanoscale data from a single cell by AFM indentation generate cellular mechanome. Such dimensional mechanome was train...
We performed ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging by using a CCD camera with fast readout time (<1 ms).The CARS signal of polystyrene bead was detected in the range 600-3600 cm -1 resolution <10 .The pixel dwell approximately 1 ms, which limited rather than exposure time.CARS images 161 × pixels were obtained polymer beads total data-acquisition 28 s even use cost-effective microchip laser source.Label-free and (18 colors) living cells also an...
The regulation of programmed cell death, or apoptosis, is crucial for normal development and the maintenance homeostasis. It has been shown that novel antiapoptotic protein Bax inhibitor-1 (BI-1) represents a new type regulator death pathways controlled by Bcl-2 Bax.Surgically resected lung specimens were obtained from 32 patients with peripheral adenocarcinomas, BI-1 gene expression was examined compared p53, bcl-2 genes.Fourteen tumors (43.8%) positive in situ hybridization. tumor...
Objective assessment of the differentiation grade hepatocellular carcinomas (HCCs) is important for evaluation pathological diagnosis, prognosis and therapeutic treatment. Differentiation hepatocytes reflected by their expression hepatic functional proteins in mouse embryo, liver‐enriched transcription factors (LETFs) have been shown to regulate genes strictly. Previous reports demonstrated that level LETF altered HCC or preneoplastic nodules compared with noncancerous tissues. Therefore,...
Atypical adenomatous hyperplasia (AAH) is classified as a precancerous lesion of lung adenocarcinoma. We established an immortalized AAH cell line (PL16T) and human non‐neoplastic bronchial epithelial (PL16B) from the same patient by transfection with gene for SV40 large T antigen. The expression profile PL16T was compared that PL16B suppression subtractive hybridization method. From 704 selectively hybridized clones, we finally selected 25 fragments mRNA showed transcription levels more...
Immunoglobulin ( CD 79a) binding protein 1 IGBP 1) is universally overexpressed in lung adenocarcinoma and exerts an anti‐apoptotic effect by to PP 2Ac. However, the molecular mechanism of overexpression still unclear. In present study, we used a micro RNA (mi ) array TargetScan Human software detect 1‐related mi s that regulate expression. The analysis revealed more than 100 are dysregulated early invasive adenocarcinoma. On other hand, silico using 79 associated with Among selected...
Fibroblasts in tumor tissue are thought to interact with cells directly and/or indirectly and have important roles invasion metastasis. To characterize the phenotype of proliferating fibroblasts pulmonary adenocarcinoma, we established short‐term fibroblast cell lines from both normal bronchus adenocarcinoma tissues obtained same patients compared gene expression profiles. Four sets (eight total) were used analysis. Total RNA was extracted each line hybridized 550 cancer‐related RNAs blotted...
S100A6 is a calcium‐binding protein implicated in many cellular processes and frequently upregulated cancer. Recently it was reported that one of the genes having higher expression adenocarcinoma mixed subtype with bronchioloalveolar carcinoma (BAC) component than pure BAC. To clarify association stepwise progression lung adenocarcinoma, examined on immunohistochemistry 92 formalin‐fixed paraffin‐embedded adenocarcinomas. Both nucleus cytoplasm tumor cells were stained, nuclear cytoplasmic...
Abstract Meridianin C is a marine natural product known for its anti‐cancer activity. At present, the anti‐tumour effects of meridianin on oral squamous cell carcinoma are unknown. Here, we investigated effect proliferation four different human tongue cancer cells, YD ‐8, ‐10B, ‐38 and HSC ‐3. Among cells tested, most strongly reduced growth ‐10B cells; aggressive tumorigenic lines tested. Strikingly, induced significant accumulation macropinosomes in confirmed by microscopic TEM analysis as...
Although lung adenocarcinoma is a major cause of cancer death worldwide, details its molecular carcinogenesis and stepwise progression are still unclear. To characterize the sequential from bronchioloalveolar (BAC, in situ carcinoma) to mixed subtype with BAC component, polymerase chain reaction‐based cDNA suppression subtractive hybridization (SSH) was carried out using two representative cases (non‐invasive tumors) (invasive tumors). Through differential screening, virtual reverse northern...
Sugita S, Morishita Y, Kano J, Furuya Shiba-Ishii A & Noguchi M(2011) Histopathology58, 729–738IGFBP-1 is expressed specifically in ovarian clear cell adenocarcinoma Aims: To investigate the specific expression of insulin-like growth factor binding protein-1 (IGFBP-1) (CCA). Methods and results: Immunohistochemistry situ hybridization for IGFBP-1 were performed normal endometrium, placenta, 100 surgically resected cases cancer including 31 CCAs 69 non-CCAs. hepatocyte nuclear factor-1...
Aberrant methylation of promoter CpG islands is known to be a major inactivation mechanism tumor-suppressor and tumor-related genes. In order identify novel hypermethylated genes in early stage lung adenocarcinoma, we carried out methylated island amplification, modified suppression subtractive hybridization, methylation-specific polymerase chain reaction aberrant the A/J mouse adenoma model, which histologically mimics human pulmonary adenocarcinoma. Through differential screening, detected...
Cholangiocarcinoma (CCA) associated with Opisthorchis viverrini (Ov) chronic infection is the most frequent primary liver cancer in Thailand, and current approaches to early diagnosis curative treatments are largely disappointing. We hypothesize a role for protein kinase A (PKA) Ov-induced CCA. First, we studied PKA isozyme switching from hamster CCA model using quantitative (q) PCR, situ hybridization, immunohistochemical western blot analysis. Second, presence of extracellular (ECPKA) cell...