A5007368328- Cytomegalovirus and herpesvirus research
- Bacterial biofilms and quorum sensing
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Toxoplasma gondii Research Studies
- Antibiotics Pharmacokinetics and Efficacy
- interferon and immune responses
- Phenothiazines and Benzothiazines Synthesis and Activities
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Viral-associated cancers and disorders
- Bacteriophages and microbial interactions
- Cancer therapeutics and mechanisms
- Microbial Community Ecology and Physiology
- Magnolia and Illicium research
- Biochemical and Structural Characterization
- DNA and Nucleic Acid Chemistry
- Orthopedic Infections and Treatments
- Bacillus and Francisella bacterial research
- Parvovirus B19 Infection Studies
- Polyomavirus and related diseases
- Oral microbiology and periodontitis research
- Oxidative Organic Chemistry Reactions
Université Laval
2024
University of Helsinki
2019-2023
Centre hospitalier universitaire de Québec
2017
A series of quaternary ammonium fluoroquinolones was obtained by exhaustive methylation the amine groups present at 7-position fluoroquinolones, including ciprofloxacin, enoxacin, gatifloxacin, lomefloxacin, and norfloxacin. The synthesized molecules were tested for their antibacterial antibiofilm activities against Gram-positive Gram-negative human pathogens, i.e. Staphylococcus aureus Pseudomonas aeruginosa. study showed that compounds are potent agents (MIC values lowest 6.25 μM) with low...
Epidemiological studies suggest that respiratory syncytial virus (RSV) and influenza A (IAV) might interfere with each other. Viral interference mainly relies on interferon production elicited by a first reduces the replication of second virus. In this paper, we investigated interactions between RSV-A2 A(H1N1)pdm09 in BALB/c mice infected single or both viruses simultaneously sequentially before, at peak after peak. IAV reduced almost 3.0 logs RSV administered induced influenza, but opposite...
ABSTRACT Human herpesviruses 6A and 6B (HHV-6A/B) can integrate their genomes into the telomeres of human chromosomes using a mechanism that remains poorly understood. To achieve better understanding HHV-6A/B integration mechanism, we made use BRACO-19, compound stabilizes G-quadruplex secondary structures prevents telomere elongation by telomerase complex. First, analyzed folding telomeric sequences binding to BRACO-19 G-quadruplex-specific antibodies surface plasmon resonance. Circular...
Some respiratory viruses can cause a viral interference through the activation of interferon (IFN) pathway that reduces replication another virus. Epidemiological studies coinfections between SARS-CoV-2 and other have been hampered by non-pharmacological measures applied to mitigate spread during COVID-19 pandemic. With ease these interventions, influenza A now co-circulate. It is thus prime importance characterize their interactions. In this work, we investigated effects an Omicron variant...
Human herpesviruses 6A/B (HHV-6A/B) can integrate their viral genomes in the telomeres of human chromosomes. The and cellular factors contributing to HHV-6A/B integration remain largely unknown, mostly due lack efficient reproducible cell culture models study integration. In this study, we characterized efficiencies several lines using two different approaches. First, after a short-term infection (5 h), cells were processed for single-cell cloning analyzed chromosomally integrated...
Human herpesvirus-6A (HHV-6A) and HHV-6B integrate their genomes into the telomeres of human chromosomes, however, mechanisms leading to integration remain unknown. HHV-6A/B encode a protein that has been proposed be involved in termed U94, an ortholog adeno-associated virus type 2 (AAV-2) Rep68 integrase. In this report, we addressed whether purified recombinant maltose-binding (MBP)-U94 fusion proteins possess biological functions compatible with viral integration. We could demonstrate...
In an effort to find new repurposed antibacterial compounds, we performed the screening of FDA-approved compounds library against Staphylococcus aureus American Type Culture Collection (ATCC) 25923. Compounds were evaluated for their capacity prevent both planktonic growth and biofilm formation as well disrupt pre-formed biofilms. One identified initial hits was fingolimod (FTY720), immunomodulator approved treatment multiple sclerosis, which then selected follow-up studies. Fingolimod...
Abstract Some respiratory viruses can cause a viral interference through the activation of interferon (IFN) pathway that reduces replication another virus. Epidemiological studies coinfections between SARS-CoV-2 and other have been hampered by non-pharmaceutical measures applied to mitigate spread during COVID-19 pandemic. With ease these interventions, influenza A now co-circulate. It is thus prime importance characterize their interactions. In this work, we investigated effects an Omicron...
In recent years, bacterial infections have become a main concern following the spread of antimicrobial resistance. addition, biofilms are known for their high tolerance to antimicrobials and they regarded as cause recalcitrant in humans. Many efforts been deployed order find new antibacterial therapeutic options high-throughput screening (HTS) large libraries compounds is one utilized strategies. However, HTS anti-biofilm discovery remain uncommon. Here, we miniaturized 96-well plate (96WP)...
Bacterial biofilms are an important underlying cause for chronic infections. By switching into the biofilm state, bacteria can evade host defenses and withstand antibiotic chemotherapy. Despite fact that at clinical environmental settings mostly composed of multiple microbial species, research has largely been focused on single-species biofilms. In this study, we investigated interaction between two clinically relevant bacterial pathogens ( Staphylococcus aureus Pseudomonas aeruginosa ) by...
Human herpesviruses 6A and 6B (HHV-6A/B) are unique among human in their ability to integrate genome into host chromosomes. Viral integration occurs at the ends of chromosomes within telomeres. The HHV-6A/B genomes contain telomeric repeats that facilitate process. Here, we report productive infections associated with a massive increase sequences viral origin. majority signals can be detected replication compartments (VRC) DNA processivity factor p41 immediate-early 2 (IE2) protein....
New classes of antibiotics are urgently needed in the fight against multidrug-resistant bacteria. Drug repurposing has emerged as an alternative approach to accelerate antimicrobial research and development. In this study, we screened a library sphingosine-1-phosphate receptor (S1PR) modulators Staphylococcus aureus identified five active compounds. Among them, etrasimod (APD334), investigational drug for treatment ulcerative colitis, displayed best inhibitory activity S. when growing...
We recently identified fingolimod as a potent antibiofilm compound by screening FDA-approved drugs. To study if the antibacterial activity of could be further improved and to explore in-depth structure–activity relationships, we synthesized 28 novel derivatives evaluated their efficacy against Staphylococcus aureus grown in planktonic/single cell biofilms. The most effective were tested on preformed S. biofilms Gram-negative bacteria Acinetobacter baumannii Pseudomonas aeruginosa, using...
Antibacterial screenings are most commonly targeted at planktonic bacteria but less effort is dedicated to the exploration of agents acting on biofilms. Here, a natural compounds library was screened against Staphylococcus aureus using 384-well plate platform identify preventing biofilm formation. Five structurally diverse hits were selected for follow-up studies: honokiol, tschimganidin, ferutinin, oridonin and deoxyshikonin. The evaluated different bacterial species their capacity prevent...
Abstract Human herpesviruses 6A and 6B (HHV-6A/B) can integrate their genomes into the telomeres of host chromosomes. The HHV-6A/B contain telomeric repeats essential for integration. Whether infections impact telomere homeostasis remains to be studied. We report that during infection, a massive increase in signals is observed. Such are detected viral replication compartments (VRC) colocalize with IE2 P41 proteins. Infection HHV-6A mutants lacking did not reproduce this phenotype. lead...