A5038634870- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Virus-based gene therapy research
- Dendrimers and Hyperbranched Polymers
- Wound Healing and Treatments
- Silk-based biomaterials and applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Crystallization and Solubility Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Escherichia coli research studies
- Retinal and Macular Surgery
- Bacterial Identification and Susceptibility Testing
- Cancer therapeutics and mechanisms
- Bacillus and Francisella bacterial research
- Viral gastroenteritis research and epidemiology
- Retinopathy of Prematurity Studies
- Skin Protection and Aging
- Barrier Structure and Function Studies
- Antimicrobial Resistance in Staphylococcus
- Echinoderm biology and ecology
- Luminescence and Fluorescent Materials
- Marine Sponges and Natural Products
University of Helsinki
2010-2024
St Petersburg University
2007-2009
Research Institute of Obstetrics and Gynecology named after D.O. Ott
2006-2007
A series of quaternary ammonium fluoroquinolones was obtained by exhaustive methylation the amine groups present at 7-position fluoroquinolones, including ciprofloxacin, enoxacin, gatifloxacin, lomefloxacin, and norfloxacin. The synthesized molecules were tested for their antibacterial antibiofilm activities against Gram-positive Gram-negative human pathogens, i.e. Staphylococcus aureus Pseudomonas aeruginosa. study showed that compounds are potent agents (MIC values lowest 6.25 μM) with low...
The development of new therapies is hampered by the lack predictive, and patient-relevant in vitro models. Organ-on-chip (OOC) technologies can potentially recreate physiological features hold great promise for tissue disease modeling. However, non-standardized design these chips perfusion control systems has been a barrier to quantitative high-throughput screening (HTS). Here we present scalable OOC microfluidic platform applied kinetic assays (AKITA) that applicable high, medium, low...
Tree stems contain wood in addition to 10–20% bark, which remains one of the largest underutilized biomasses on earth. Unique macromolecules (like lignin, suberin, pectin, and tannin), extractives, sclerenchyma fibers form main part bark. Here, we perform detailed investigation antibacterial antibiofilm properties bark-derived fiber bundles discuss their potential application as wound dressing for treatment infected chronic wounds. We show that yarns containing at least 50% willow bark...
The development of a mild and green method for separating natural fiber bundles from willow bark is an essential step in exploring preserving their functions. isolation well-oriented the fast-growing hybrid solely using sodium bicarbonate under conditions was successfully demonstrated. Additionally, Lyocell fibers were mixed with equal amount proved ability to convert into spun yarns, which provided excellent protection ultraviolet radiation (UPF ≥ 140). Moreover, these yarns demonstrated...
Hundreds of different fast-growing Salix hybrids have been developed mainly for energy crops. In this paper, we studied water extracts from the bark 15 willow and species as potential antimicrobial additives. Treatment ground in under mild conditions extracted 12-25% dry material. Preparative high-performance liquid chromatography is proven here a fast highly efficient tool small-scale recovery raffinose crude structural elucidation. Less than half dissolved material was assigned by...
Since quorum sensing (QS) is linked to the establishment of bacterial infection, its inactivation represents one newest strategies fight pathogens. LsrK a kinase playing key role in processing autoinducer-2 (AI-2), quorum-sensing mediator gut enteric bacteria. Inhibition might thus impair cascade and consequently reduce pathogenicity. Aiming for development target-based assay discovery inhibitors, we evaluated different set-ups based on ATP detection optimized an automation-compatible method...
For long-term live-cell fluorescence imaging and biosensing, it is crucial to work with a dye that has high quantum yield photostability without being detrimental the cells. In this paper, we demonstrate neutral boron-dipyrromethene (BODIPY)-based molecular rotors have great properties for high-light-dosage demanding applications require repetitive illuminations. rotors, an intramolecular rotation (IMR) allows alternative route decay of singlet excited state (S1) via formation charge...
The continuing emergence and spread of antibiotic-resistant bacteria is worrisome new strategies to curb bacterial infections are being sought. interference quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. AI-2 QS system present in multiple species shown be correlated with pathogenicity. To facilitate the discovery novel compounds interfering QS, we established high-throughput setup whole-cell bioreporter assay, which can performed either 96- or...
The first total synthesis of the marine bromotyrosine purpurealidin I (1) using trifluoroacetoxy protection group and its dimethylated analog (29) is reported along with 16 simplified derivatives lacking tyramine moiety. Their cytotoxicity was evaluated against human malignant melanoma cell line (A-375) normal skin fibroblast cells (Hs27) together 33 purpurealidin-inspired amides, structure–activity relationships were investigated. synthesized analogs without part retained cytotoxic...
Marine-originated spirocyclic bromotyrosines are considered as promising scaffolds for new anticancer drugs. In a continuation of our research to develop potent and more selective compounds, we synthesized library 32 clavatadine analogs by replacing the agmatine, i.e., 4-(aminobutyl)guanidine, side chain with different substituents. These compounds were tested cytotoxicity against skin cancer using human melanoma cell line (A-375) normal fibroblast (Hs27). The highest A-375 was observed...
Abstract Treatment of retinal diseases currently demands frequent intravitreal injections due to rapid clearance the therapeutics. The use high molecular weight polymers can extend residence time in vitreous and prolong injection intervals. This study reports a water soluble graft copolymer as potential vehicle for sustained drug delivery. features hyaluronic acid (HA) backbone poly(glyceryl glycerol) (PGG) side chains attached via hydrolysable ester linkers. PGG, polyether with 1,2‐diol...
Enteropathogenic E. coli (EPEC) causes intestinal infections leading to severe diarrhea. EPEC attaches the host cell causing lesions epithelium coupled with effacement of microvilli. In process, actin accumulates into a pedestal-like structure under bacterial microcolonies. We designed an automated fluorescence microscopy-based screening method for discovering compounds capable inhibiting adhesion and virulence using aurodox, type three secretion system (T3SS) inhibitor, as positive control....
Abstract Uropathogenic Escherichia coli , the most common cause for urinary tract infections, forms biofilm enhancing its antibiotic resistance. To assess effects of compounds on formation uropathogenic UMN026 strain, a high-throughput combination assay using resazurin followed by crystal violet staining was optimized 384-well microplate. Optimized parameters included, example, and concentrations, incubation time readouts. For validation, quality Z′ factor, coefficient variation,...
Cryptophytes are a promising source of bioactive compounds that have not been fully explored. This research investigated the antimicrobial activity total phenolic (TPC) and exopolysaccharides (EPS) extracted from several cryptophytes against range harmful foodborne bacteria fungi. To measure minimum inhibitory concentration (MIC) value, broth microdilution method was used. In antibacterial evaluation TPC, MIC ranged between 31.25 500 μg/mL, while for antifungal test, it varied to 125 μg/mL....
Development of effective non-viral DNA carriers is considered to be a perspective approach for delivery into eucaryotic cells with gene therapy aims. Our research was devoted group asymmetric lysine dendrimers. We studied fifth generation dendrimer D5 and its analog D5C10 which modified by capric acid residues in external sphere. All vehicles were investigated their capacity bind (using gel-retardation ethidium bromide exclusion assay) provide protection plasmid from nuclease degradation...
As a continuation of our previous investigations aimed at the synthesis novel nitrogen-containing heterocycles and their metal complexes, we have now prepared two series compounds incorporating phthalazine ring position C2 4,5-dihydro-1H-imidazole. The starting (I) in reaction with 2-chloroimidazoline (II) gives rise to formation pseudobase III. Then, compound III upon treatment HOSA yields betaine which under basic conditions 2-(4,5-dihydro-1H-imidazol-2-yl)phthalazin-1(2H)-imine (IV). In...