A5102920211- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Exercise and Physiological Responses
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Memory and Neural Mechanisms
- Developmental Biology and Gene Regulation
- COVID-19 epidemiological studies
- COVID-19 diagnosis using AI
- Physical Activity and Health
- Spinal Cord Injury Research
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Data-Driven Disease Surveillance
- Single-cell and spatial transcriptomics
- Aquaculture disease management and microbiota
- Advanced Image Fusion Techniques
- Insect and Arachnid Ecology and Behavior
- Remote-Sensing Image Classification
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Genetics and Physical Performance
- Advanced Image Processing Techniques
National Cheng Kung University
2024-2025
Des Moines University Osteopathic Medical Center
2022-2024
Research Center for Applied Science, Academia Sinica
2019-2022
Des Moines University
2022
Academia Sinica
2021
University of California, Los Angeles
2018-2020
University of California, Berkeley
2012-2015
Institute of Biomedical Sciences, Academia Sinica
2004-2013
National Defense Medical Center
2006-2012
Significance Brain-derived neurotrophic factor (BDNF) is a secreted neurotrophin known to mediate activity-dependent synaptic plasticity. Endogenously synthesized BDNF normally stored and transported in dense core vesicles at synapses response activity. However, may also be endocytosed by neurons within neuronal cytoplasm the form of endosomes. By monitoring bound fluorescent quantum dots (BDNF-QDs), we found that BDNF-QDs could preferentially localized postsynaptic sites cultured...
Hyperspectral image (HSI) fusion addresses the challenge of reconstructing High-Resolution HSIs (HR-HSIs) from Multispectral images (HR-MSIs) and Low-Resolution (LR-HSIs), a critical task given high costs hardware limitations associated with acquiring high-quality HSIs. While existing methods leverage spatial spectral relationships, they often suffer limited receptive fields insufficient feature utilization, leading to suboptimal performance. Furthermore, scarcity HSI data highlights...
Significance The tamoxifen (TAM)-inducible CreER/LoxP system is one of the most widely used genetic tools that have enabled precise gene manipulation in distinct cell subpopulations at any specific time point and labeling types for fate mapping. We report administration TAM during early stages cortical neurogenesis inhibits neural progenitor proliferation impaired neurogenesis, dendritic morphogenesis, synaptic density, gliogenesis postnatal offspring. By employing single-cell RNA sequencing...
Regular exercise has numerous health benefits, but the human population displays significant variability in participation. Rodent models, such as voluntary wheel running (VWR) rats, can provide insight into underlying mechanisms of behavior and its regulation. In this study, we focused on role estrogen VWR female rats. Female rats run more than males, aimed to determine what extent levels females were regulated by signaling. The (duration, speed, total distance run) was measured under normal...
Abstract The absence of a slice preparation with intact thalamocortical pathways has held back elucidation the cellular and synaptic mechanisms by which thalamic signals are differentially transmitted to processed in anterior cingulate cortex (ACC). In this report we introduce an innovative mouse brain it is possible explore electrophysiological properties ACC neurons long‐distance inputs from medial (MT) nuclei intracellular recordings; MT–ACC neuronal pathway plays integral role...
Purpose: Seizure-like activities generated in anterior cingulate cortex (ACC) are usually classified as simple partial and associated with changes autonomic function, motivation, thought. Previous studies have shown that thalamic inputs can modulate ACC seizure, but the exact mechanisms not been studied thoroughly. Therefore, we investigated role of modulating seizure-like activities. In addition, seizure onset propagation difficult to determine vivo ACC. We spatiotemporal epileptiform...
It is highly challenging to evaluate the species’ content and behavior changes in wild fireflies, especially for a sympatric population. Here, flash interval (FI) duration (FD) of flying males from three species (Abscondita cerata, Luciola kagiana, curtithorax) were investigated their potentials assessing composition nocturnal behaviors during A. cerata mating season. Both FI FD quantified continuous flashes adult fireflies (lasting 5–30 s) via spatiotemporal analyses video recorded along...
Prominent 7–12 Hz oscillations in frontal cortical networks rats have been reported. However, the mechanism of generation and physiological function this brain rhythm not yet clarified. Multichannel extracellular field potentials ACC were recorded analyzed using current source density method halothane-anesthetized rats. Spontaneous high-current spikes (HCSs) localized deep part layer II/III upper V ACC. The frequency HCSs was Hz, with an amplitude 6.5 ± 0.76 mV/mm<sup>2</sup> duration 55.24...
The mammalian central nervous system (CNS) is considered an immune privileged as it separated from the periphery by blood brain barrier (BBB). Yet, functions have been postulated to heavily influence functional state of CNS, especially after injury or during neurodegeneration. There controversy regarding whether adaptive responses are beneficial detrimental CNS repair. In this study, we utilized immunocompromised SCID mice and subjected them spinal cord (SCI). We analyzed motor function,...
More and more studies have focused on the treatment of spinal cord injury (SCI) by tissue engineering, but there is still no ideal animal model that can genuinely objectively simulate real pathological process in clinical practice. Also, given increasing availability use genetically modified animals basic science research, it has become essential to develop clinically related models for SCI mice.Forty-eight C57BL/6 mice were divided into three groups (injured/sham/uninjured). We determined...
In the zebrafish lateral line system, interneuromast cells (INCs) between neuromasts are kept quiescent by underlying Schwann (SWCs). Upon severe injuries that cause complete loss of an entire neuromast, INCs can occasionally differentiate into but how they escape from inhibition SWCs is still unclear. Using a genetic/chemical method to ablate neuromast precisely, we found small portion larvae regenerate new neuromast. However, residual regeneration capacity was hindered inhibiting...
Conventional Computed Tomography (CT) imaging recognition faces two significant challenges: (1) There is often considerable variability in the resolution and size of each CT scan, necessitating strict requirements for input adaptability models. (2) CT-scan contains large number out-of-distribution (OOD) slices. The crucial features may only be present specific spatial regions slices entire scan. How can we effectively figure out where these are located? To deal with this, introduce an...
Introduction This study investigates the relationship between gut microbiome and voluntary exercise, focusing on wheel running activity in a rat model. The plays crucial role host physiology, homeostasis, behavior. Alterations have been linked to various pathological states health conditions, including obesity. Methods Given strong association physical inactivity obesity development, our aimed identify factors associated with elevated levels of exercise. Male Sprague Dawley rats were used...
Summary CreER/LoxP system has enabled precise gene manipulation in distinct cell subpopulations at any specific time point upon tamoxifen (TAM) administration. This is widely accepted to track neural lineages and study functions. We have observed prenatal TAM treatment caused high rate of delayed delivery mortality pups. These substances could promote undesired results, leading data misinterpretation. Here, we report that administration during early stages cortical neurogenesis promoted...
Despite the myriad social and health benefits of exercise, humans display heterogeneous levels participation. Significant progress has been made in identifying molecular events, systems, mechanisms that support exercise's beneficial effects, but it is not clear yet what regulates exercise behavior itself or serves to maintain prolonged chronic behavior. The human heterogeneity voluntary can be recapitulated a rodent model wheel running, with high rewarding properties. While rats given...
Literature establishes conserved changes in the gut microbiome (GMB) correlated with behavior.1 Using voluntary running, we tested if GMB variability explains differences exercise using a rat model.Cohabitating male rats were randomized into running (n=8) and sedentary (n=3) groups placed individual cages. Running wheel access was withheld for week. The group received free to wheels 27 days. Daily cumulative distances recorded weekly fecal collection 16s amplification sequencing. Terminal...