A5063673623- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Lung Cancer Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Microscopic Colitis
- Gastrointestinal motility and disorders
- CAR-T cell therapy research
- Head and Neck Cancer Studies
- Protein Kinase Regulation and GTPase Signaling
- SARS-CoV-2 detection and testing
- IL-33, ST2, and ILC Pathways
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Plant Molecular Biology Research
- Plant responses to water stress
- Family Support in Illness
- Schizophrenia research and treatment
- Vector-borne infectious diseases
- Colorectal and Anal Carcinomas
- Cellular transport and secretion
- Cervical Cancer and HPV Research
- Plant Stress Responses and Tolerance
- Inflammatory Bowel Disease
University of Vermont
2003-2023
Baltimore Washington Center for Psychoanalysis
2022
University of Vermont Medical Center
2021
Queensland University of Technology
2020
Imperial College Healthcare NHS Trust
2019
Vermont Department of Health
1991
DuPont (United States)
1952
Abstract H2O2 is a widespread molecule in many biological systems. It created enzymatically living cells during various oxidation reactions and by leakage of electrons from the electron transport chains. Depending on concentration can induce cell protective responses, programmed death, or necrosis. Here we provide evidence that may function as developmental signal differentiation secondary walls cotton (Gossypium hirsutum) fibers. Three lines support this conclusion: (a) period generation...
Fecal microbiota transplantation (FMT) is a promising new strategy in the treatment of Inflammatory Bowel Disease, but long-term delivery systems are lacking. This randomized study was designed as safety and feasibility FMT subjects with mild to moderate UC using frozen, encapsulated oral (cFMT).Subjects were 1:1 receive induction by colonoscopy, followed 12 weeks daily administration frozen cFMT or sham therpay. Subjects for 36 longitudenal clinical assessments included multiple subjective...
For fecal microbiota transplantation (FMT) to be successful in immune diseases like inflammatory bowel disease, it is assumed that therapeutic microbes and their beneficial functions interactions must colonize a recipient patient persist sufficient quantity for period of time produce clinical benefit. Few studies, however, have comprehensively profiled the colonization persistence transferred along with transfer microbial host system. Using 16S, metagenomic, immunoglobulin A (IgA)...
Signal transduction through the Rho family GTPases requires regulated cycling of between active GTP-bound state and inactive GDP-bound state. members containing an arginine residue at position 186 in C-terminal polybasic region were found to possess a self-stimulatory GTPase-activating protein (GAP) activity homophilic interaction, resulting significantly enhanced intrinsic GTPase activities. This functions effectively as "arginine finger" activating reaction confer catalytic GAP but is not...
Abstract Activation of the innate immune system typically precedes engagement adaptive immunity. Cells at interface between these two arms response are thus critical to provide full host defense. Among T cells this γδ cells. contribute defense from a variety infectious organisms, yet little is understood regarding how they activated. We have previously observed that human Vδ1 subset accumulate in inflamed joints Lyme arthritis and proliferate stimulation with causative spirochete, Borrelia...
The Saccharomyces cerevisiae Cdc42p GTPase is localized to the plasma membrane and involved in signal transduction mechanisms controlling cell polarity. of action dominant negative cdc42 D118Amutant lethal, gain functioncdc42 G12V mutant were examined. Cdc42D118A,C188Sp its guanine-nucleotide exchange factor Cdc24p displayed a temperature-dependent interaction two-hybrid system, which correlated with temperature dependence D118A phenotype supported sequestration model for mechanism ofcdc42...
Abstract γδ T cells participate in the innate immune response to a variety of infectious microorganisms. They also link adaptive through their induction maturation dendritic (DC) during early phase an when frequency Ag-specific is very low. We observe that presence Borrelia burgdorferi, synovial Vδ1 from Lyme arthritis fluid potently induce DC, including production IL-12, and increased surface expression CD40 CD86. The activated DC are then able stimulate up-regulate CD25. Both these...
People with severe mental illness (SMI) experience poor physical health and premature mortality, contributed significantly by modifiable lifestyle risk factors such as nutrition, low cardiorespiratory fitness, inactivity. Lifestyle interventions can reduce cardiometabolic confer a range of other positive benefits. We assessed the feasibility, acceptability, safety, preliminary effectiveness (combined dietary exercise) intervention lead senior exercise dietetics students in residential...
An effective adaptive immune response requires rapid T cell proliferation, followed by equally robust death. These two processes are coordinately regulated to allow sufficient magnitude of its resolution, while also providing the maintenance memory. Both aspects this characterized profound changes in metabolism; glycolysis drives proliferation whereas oxidative phosphorylation supports survival memory cells. While much is known about separate expansion and contraction, considerably less...
γδ T cells function at the interface between innate and adaptive immunity have well-demonstrated roles in response to infection, autoimmunity tumors. A common characteristic of these seemingly disparate conditions may be cellular stress or death. However, under which ligands for are induced exposed remain largely undefined. We observed that induction necroptosis murine human dendritic (DC) by inhibition caspase activity paradoxically augments their ability activate cells....
Abstract For fecal microbiota transplantation (FMT) to be successful in complex immune diseases like inflammatory bowel disease (IBD), it is assumed that therapeutic microbes and their beneficial functions interactions must colonize the recipient persist sufficient quantity for a long enough period of time result clinical benefit. But few studies have comprehensively profiled colonization persistence transferred along with transfer microbial functions. Using 16S, metagenomic, immunoglobulin...
Lack of understanding the nature and physiological regulation γδ T cell ligands has considerably hampered full function these cells. We developed an unbiased approach to identify human cells by production a soluble TCR-γδ (sTCR-γδ) tetramer from synovial Vδ1 clone Lyme arthritis patient. The sTCR-γδ was used in flow cytometry initially define spectrum ligand expression both tumor lines certain primary Analysis diverse revealed high on several epithelial or fibroblast origin, whereas those...
γδ T cells reside at mucosal and epithelial barriers, they often accumulate sites of inflammation, both infectious autoimmune, as well in certain tumors. However, progress understanding their function is considerably hampered by a lack full the ligands recognized TCR-γδ how expression these regulated. We recently developed soluble human (Vγ9Vδ1) tetramer from synovial cell clone Lyme arthritis patient observed that it stains monocytes activated Borrelia burgdorferi. Those findings are...
Abstract Background Fecal microbiota transplantation (FMT) is a promising new strategy in the treatment of Inflammatory Bowel Disease, but long-term delivery systems are lacking. This randomized study was designed as safety and feasibility fecal subjects with mild to moderate UC using frozen, encapsulated oral FMT (cFMT). Methods Subjects were 1:1 receive induction by colonoscopy, followed 12 weeks daily administration frozen cFMT or sham therpay. for 36 longitudenal clinical assessments...
Abstract This randomized study was designed to assess the safety and feasibility of long-term fecal microbiota transplantation (FMT) in subjects with mild moderate UC using frozen, encapsulated oral FMT (cFMT). induction implemented by colonoscopy, followed 12 weeks daily administration frozen cFMT. Ribosomal 16S bacterial sequencing used donor-induced changes gut microbiota. Changes T regulatory (Treg) mucosal associated invariant (MAIT) cell populations were evaluated as an exploratory...
ABSTRACTABSTRACTThis clinical paper expounds upon the well-established utility of transference interpretations in general and specifically delineates resistances to their use; that arose because analysand’s traumatic loss her mother. The timing violent nature mother’s death highlighted developmental relevance premorbid, triangulated dynamic with parents. That is, opportunity uncomplicated development affords regard working through one’s pre- post-oedipal conflicts was unavailable her....
The 2-year-old daughter of two farm laborers was reported missing while the owner harvesting corn. Unidentifiable tissues and body parts were subsequently found admixed with silage. Samples blood collected from parents child as well portions tissue recovered silage subjected to analysis DNA polymorphisms probes usually used identify paternity. In addition, allele-specific oligonucleotides detect polymorphism at DQ α locus following amplification using polymerase chain reaction. this case,...
Abstract γδ T cells function between the innate and adaptive immune responses, promoting antigen-presenting cell function, presenting antigen, manifesting cytolytic activity. Their numbers increase in infections, such as HIV, at sites of chronic inflammation. However, turnover dynamics human are poorly understood. Here we find that despite more rapid proliferation, Lyme arthritis synovial Vδ1 subset have reduced number expansion vitro compared to αβ due increased death by cells. Because...
<h3>Background</h3> Pulmonary rehabilitation (PR) is recognized as a core component of the management patients with COPD.<sup>1</sup> Although benefits PR in COPD are well established, there remain number unanswered questions regarding how to maximise performance during PR, including use ambulatory oxygen. Studies investigating effects oxygen have had conflicting results.<sup>2</sup> Therefore, we aimed investigate effect on outcomes <h3>Methods</h3> Patients referred and exercise...