A5027659841- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Genomics and Rare Diseases
- Advanced Nanomaterials in Catalysis
- Metabolism and Genetic Disorders
- Iron Metabolism and Disorders
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects and Toxicity Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Liver Disease Diagnosis and Treatment
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- Amino Acid Enzymes and Metabolism
- Hemoglobinopathies and Related Disorders
- Biochemical Analysis and Sensing Techniques
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Biotechnology and Related Fields
- Advanced Proteomics Techniques and Applications
- Analytical chemistry methods development
- Corporate Finance and Governance
- Autopsy Techniques and Outcomes
- Carbohydrate Chemistry and Synthesis
- RNA modifications and cancer
- Ethics in Clinical Research
North-West University
2012-2025
University of Pretoria
2006-2007
Abstract King-Denborough Syndrome (KDS) is a congenital myopathy (CM) characterised by myopathy, dysmorphic features and susceptibility to malignant hyperthermia. The objective of this study was investigate the genotype-phenotype correlation in Black African patients presenting with CM, specifically those KDS-like phenotypes, who remained undiagnosed for over 25 years. A cohort 67 CM studied, whom 44 were clinically evaluated diagnosed KDS. Whole-exome sequencing (WES) performed as part an...
Gold nanostars (AuNSs) are seen as promising building blocks for biosensors with potential easy readouts based on naked-eye and ultraviolet–visible spectroscopy detection. We present a seedless synthesis strategy AuNSs that has the advantages of seeded methods. The method used ascorbic acid reducing agent silver nitrate an anisotropic growth control assisting agent. multiple branches diameter 59 nm were produced. They showed good stability when capped PVP modified enzyme in relatively strong...
Worldwide, comprehensive newborn screening (NBS) now includes a clinical examination at birth, hearing screening, pulse oximetry measurement for congenital heart defects, and biochemical to identify disorders early in life, preventing irreversible damage, mortality enhancing overall health outcomes. This article provides overview of NBS South Africa, outlining the history, current status, future plans expansion. In is fragmented, with some investigations included neonatal assessments....
Low arginine bioavailability is associated with vaso-occlusive painful crisis (VOC) severity in sickle cell anemia (SCA) and predicts need for pediatric hospitalization. Intravenous therapy has opioid-sparing effects was found to significantly decrease pain scores children hospitalized SCA-VOC a phase-two randomized placebo-controlled trial (RCT). Efficacy of oral unknown. Our objective determine the safety efficacy Nigerian SCA. A double-blind RCT L-arginine-hydrochloride (100 mg/kg TID)...
Gold nanoparticles provide a user-friendly and efficient surface for immobilization of enzymes proteins. In this paper, we present novel approach enzyme bioconjugation to gold nanostars (AuNSs). AuNSs were modified with l-cysteine (Cys) covalently bound N-hydroxysulfosuccinimide (sulfo-NHS) activated intermediate glucose oxidase (GOx) fabricate stable sensitive AuNSs-Cys-GOx bioconjugate complex. Such strategy has the potential increased attachment affinity without protein adsorption onto...
Gold nanostars are being used more regularly in the biosensing field. Despite their useful attributes, there is still a need to optimize aspects of synthesis and stability. The seedless, synthetic method comprising 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) facile, rapid method; however, it produces heteromorphic nanostars. modification HEPES resulted silver-assisted, seedless gold nanostar method. resulting from this were monodispersed, multi-branched approximately 37 ± 2 nm...
Capping agents (organic ligands, polymers, and surfactants) are pivotal for stabilizing nanoparticles; however, they may influence the surface chemistry, as well physico-chemical biological characteristics, of gold nanostar (AuNS)-based biosensors. In this study, we proved that various capping affected capped bioconjugated AuNS stability, functionality, biocatalysis, colorimetric readouts. Capped AuNSs were applied localized plasmon resonance (LSPR)-based H2O2 sensors using glucose oxidase...
Abstract Background Multiple acyl-CoA dehydrogenase deficiency (MADD) is an autosomal recessive disorder resulting from pathogenic variants in three distinct genes, with most of the occurring electron transfer flavoprotein-ubiquinone oxidoreductase gene ( ETFDH) . Recent evidence potential founder for MADD South African (SA) population, initiated this extensive investigation. As part International Centre Genomic Medicine Neuromuscular Diseases study, we recruited a cohort patients diagnosed...
Gold nanostars are being utilized more regularly in the field of nanodiagnostics. The modified seedless synthetic method comprised 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) synthesized with addition silver nitrate was applied for a biosensor application. colorimetric ability these newly showed to be sensitive and visually colorful than HEPES gold without nitrate. It observed that nanostar assay could tailored specific application using either hydroxylamine or sodium...
ALG6-CDG (formerly named CDG-Ic) (phenotype OMIM 603147, genotype 604566), is caused by defective endoplasmic reticulum α-1,3-glucosyltransferase (E.C 2.4.1.267) in the N-glycan assembly pathway (Grünewald et al. 2000). It second most frequent N-glycosylation disorder after PMM2-CDG; some 37 patients have been reported with 21 different ALG6 gene mutations (Haeuptle & Hennet 2009; Al-Owain 2010). We report on clinical and biochemical findings of five novel Caucasian South African patients....
Diabetes Mellitus is a growing global concern. The current methods used to detect glycated haemoglobin are precise, however, utilise expensive equipment, reagents and consumables. These luxuries which rural communities cannot access. nanotechnology have been developed for detection predominantly electrochemically based, complicated lengthy fabrication processes toxic chemicals. Here fructosyl amino acid oxidase gold nanostar biosensor has as potential future point of care candidate...
Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with characteristic dysregulated metabolism. Abnormal clinicopathological features linked to defective metabolic and inflammatory response pathways can induce PDAC development progression. In this study, we investigated the metabolites lipoproteins profiles of patients African ancestry. Nuclear Magnetic Resonance (NMR) spectroscopy was conducted on serum obtained from consenting individuals (34 PDAC, 6 Chronic Pancreatitis, healthy...
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Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to clustering after being functionalised oxidase (GOx). The quantified serum samples 1:1000 dilution factor, and colorimetrically distinguished between concentrations. assay...
The developing world is seeing rapid growth in the availability of biological mass spectrometry (MS), particularly through core facilities. As proteomics and metabolomics becomes locally feasible for investigators these nations, application areas associated with high burden such as infectious disease, will see greatly increased research output. This article evaluates MS South Africa (currently approaching 20 laboratories) a model establishing facilities other nations world. Facilities should...
ABSTRACT: A validated gas chromatographic–mass spectrometric method for quantitative analysis of methaqualone (MTQ) in illicit preparations is reported. The proved to have a coefficient variation below 5%. Four batches seized tablets, two pairs with similar imprints, were analyzed. It was found that the average MTQ concentration all four tablets differed significantly ( p =0.01) rendering it impossible conclude that, on basis alone, logo originated from same manufacturer or manufacturing...
The aim of this study was to improve the extraction method for urinary organic acids by miniaturizing and automating process. Currently, manual methods are commonly used, which can be time-consuming lead variations in test results. To address these issues, we reassessed miniaturized in-house method, reducing number steps sample-to-solvent volumes required. evaluated translated into an automated procedure on a MicroLab (ML) Star (Hamilton Technologies) liquid handler. This then validated...
We describe a competitive colorimetric assay that enables rapid and sensitive detection of galactose reduced nicotinamide adenine dinucleotide (NADH) via readouts demon-strates its usefulness for monitoring NAD+-driven enzymatic reactions. present plasmonic sensing approach assessing concentration the presence NADH using dehydrogenase immobilized gold nanostars (AuNS-PVP-GalDH). The AuNS-PVP-GalDH remains turquoise blue in absence NADH; however, as concentrations grow, reaction well color...