A5032231738- Microbial Metabolic Engineering and Bioproduction
- Insect and Pesticide Research
- Plant and animal studies
- Plant biochemistry and biosynthesis
- Insect and Arachnid Ecology and Behavior
- Lipid metabolism and biosynthesis
- Insect-Plant Interactions and Control
- Heavy Metals in Plants
- Plant Parasitism and Resistance
- Medicinal plant effects and applications
- Click Chemistry and Applications
- Protein Structure and Dynamics
- Neurobiology and Insect Physiology Research
- Transgenic Plants and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Phytochemicals and Antioxidant Activities
- Metalloenzymes and iron-sulfur proteins
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2017-2023
Czech Academy of Sciences
2018-2023
Charles University
2017-2023
Fatty acyl reductases (FARs) are involved in the biosynthesis of fatty alcohols that serve a range biological roles. Insects typically harbor numerous FAR gene family members. While some FARs pheromone biosynthesis, significance large number insect genomes remains unclear. Using bumble bee (Bombini) expression analysis and functional characterization, hymenopteran tree reconstruction, inspection transposable elements (TEs) genomic environment FARs, we uncovered massive expansion Hymenoptera,...
Abstract Termites are dominant animals of tropical terrestrial ecosystems. Their success is due to their eusocial organization as well ability digest dead plant tissues. While being extremely abundant, the termite diet poor in crucial nutrients, such fatty acids. Linoleic acid (LA) a precursor for many vital biomolecules, and most depend on its dietary supply. count among exceptions known produce LA de novo, presumably via action an unknown Δ12 acyl desaturase (FAD) introducing second double...
Fatty alcohols (FA-OH) are aliphatic unbranched primary with a chain of four or more carbon atoms. Besides potential industrial applications, fatty have important biological functions as well. In nature, produced part mixture pheromones in several insect species, such moths, termites, bees, wasps, etc. addition, FA-OHs for agricultural example, they may be used suitable substitute commercial insecticides. The insecticides drawbacks associated their preparation, and exert negative impact on...
Membrane fatty acyl desaturases (mFAD) are ubiquitous enzymes in eukaryotes. They introduce double bonds into acids (FAs), producing structurally diverse unsaturated FAs which serve as membrane lipid components or precursors of signaling molecules. The mechanisms controlling enzymatic specificity and selectivity desaturation are, however, poorly understood. We found that the physicochemical properties, particularly side chain volume, a single amino acid (aa) residue insect mFADs...
A recently proposed reaction mechanism of soluble Δ9 desaturase (Δ9D) allowed us to identify auxiliary residues His203, Asp101, Thr206 and Cys222 localized near the di-iron active site that are supposedly involved in proton transfer (PT) from site. The PT, along with electron (ET), seems be crucial for efficient desaturation. Thus, perturbing major PT chains is expected impair native (potentially) amplify minor channels, such as substrate hydroxylation. To verify this hypothesis, we mutated...
One of the most interesting groups fatty acid derivates is group conjugated acids from which researched include: linoleic (CLA) and linolenic (CLNA), are associated with countless health benefits. Sex pheromone mixtures some insect species, including tobacco horn-worm (Manduca sexta), typical for production uncommon C16 long two three double bonds, as opposed to C18 CLA CLNA. In this study, M. sexta desaturases MsexD2 MsexD3 were expressed in multiple strains Y. lipolytica different...
Abstract The conserved fatty acyl reductase (FAR) family is involved in biosynthesis of alcohols that serve a range biological roles. In moths, butterflies (Lepidoptera), and bees (Hymenoptera), FARs biosynthesize alcohol pheromones participating mate-finding strategies. Using combination next-generation sequencing, analysis transposable elements (TE) the genomic environment FAR genes, functional characterization from Bombus lucorum, B. lapidarius , terrestris we uncovered massive expansion...
One of the most interesting groups fatty acid derivates is group conjugated acids, from which researched are linoleic (CLA) and linolenic (CLNA), associated with countless health benefits. Sex pheromone mixture some insect species, including tobacco horn-worm (Manduca sexta), typical for production uncommon C16 long acids two three double bonds, as opposed to C18 CLA CLNA. In this study, M. sexta desaturases MsexD2 MsexD3 were expressed in multiple strains Y. lipolytica different genotypes....