A5047013776- Plant Virus Research Studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Cancer therapeutics and mechanisms
- Phytase and its Applications
- Transgenic Plants and Applications
- Biotin and Related Studies
- Plant Disease Resistance and Genetics
- Mitochondrial Function and Pathology
- Phytochemicals and Antioxidant Activities
- GABA and Rice Research
- Insect and Pesticide Research
- Plant Pathogens and Resistance
- Cancer Treatment and Pharmacology
- Plant pathogens and resistance mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Plant responses to elevated CO2
- Pharmacogenetics and Drug Metabolism
- Sperm and Testicular Function
- Studies on Chitinases and Chitosanases
Charles University
2015-2024
Analytical Engineering (United States)
2012-2013
Czech Academy of Sciences
2007
Czech Academy of Sciences, Institute of Physics
2007
Czech Academy of Sciences, Institute of Microbiology
2007
Abstract Ellipticine is a potent antineoplastic agent, whose mode of action considered to be based mainly on DNA intercalation, inhibition topoisomerase II and cytochrome P450‐mediated formation covalent adducts. This the first report molecular mechanism ellipticine oxidation by peroxidases (human myeloperoxidase, human ovine cyclooxygenases, bovine lactoperoxidase, horseradish peroxidase) species forming ellipticine‐DNA Using NMR spectroscopy, structures 2 metabolites were identified; major...
The influence of viral infection caused by two different potyviruses, Potato virus Y (PVY) and A (PVA) on plant metabolism photosynthetic apparatus Nicotiana tabacum L. cv. Samsun Petit Havana SR1 was studied. main stress focused the activities phosphoenolpyruvate carboxylase (PEPC), NADP-malic enzyme (NADP-ME), pyruvate phosphate dikinase (PPDK). analysis presence proteins, activities, parameters showed time dependent progress NADP-ME PEPC activities. PVY significant response, while PVA...
Fungal β‐ N ‐acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship other fungal hexosaminidases. It high GalNAcase activity, tolerates substrates the modified N‐acyl group better and some unusual catalytic properties. In order to understand these features, we performed isolation, biochemical enzymological characterization, molecular...
The best-characterized functional motifs of the potyviral Helper-Component protease (HC-Pro) responding for aphid transmission, RNA silencing suppression, movement, symptom development, and replication are gathered in this review. potential cellular protein targets plant virus proteases remain largely unknown despite their multifunctionality. HC-Pro catalytic domain, as a cysteine protease, autoproteolytically cleaves polyproteins sequence motif YXVG/G is not expected to act on host targets;...
Salt stress is one of the most damaging plant stressors, whereas hypoosmotic not considered to be a dangerous type in plants and has been less extensively studied. This study was performed compare metabolism cucumber grown soil with transferred distilled water 100 mM NaCl solution. Even though caused by did cause such significant changes relative content, Na+/K+ ratio Rubisco content as those salt stress, it accompanied more pronounced specific activities NADP-dependent enzymes. After 3...
Fungal β-N-acetylhexosaminidases catalyze the hydrolysis of chitobiose into its constituent monosaccharides. These enzymes are physiologically important during life cycle fungus for formation septa, germ tubes and fruit-bodies. Crystal structures known two monomeric bacterial dimeric human lysosomal β-N-acetylhexosaminidase. The fungal robust commonly used in chemoenzymatic syntheses oligosaccharides. enzyme from Aspergillus oryzae was purified sequence determined. complete primary structure...
Pythium oligandrum is a unique biological control agent. This soil oomycete not only acts as mycoparasite, but also interacts with plant roots and stimulates defense response via specific elicitors. In addition, P. can synthetize auxin precursors stimulate growth. We analyzed the secretomes biochemical properties of eleven isolates to find novel effective strain advantageous features for plants. Our results showed that even closely related significantly differ in content compounds secreted...
Filamentous fungi produce and secrete β-N-acetylhexosaminidases, Hex, as important components of the binary chitinolytic systems involved in formation septa hyphenation. Enzyme reconstitution experiments published previously indicate that Hex can occur form two molecular species containing either one or molecules propeptide noncovalently associated with enzyme dimer. Here, we describe a novel mechanism for regulation activity based on association their catalytic subunits large N-terminal...