A5086178845- Acute Kidney Injury Research
- Cardiac Ischemia and Reperfusion
- Organ Transplantation Techniques and Outcomes
- Phagocytosis and Immune Regulation
- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Anesthesia and Neurotoxicity Research
- Nitric Oxide and Endothelin Effects
- Renal and related cancers
- Immune cells in cancer
- Cardiac Arrest and Resuscitation
- Clinical Nutrition and Gastroenterology
- Electrical and Bioimpedance Tomography
- Complement system in diseases
- Dialysis and Renal Disease Management
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Renal Transplantation Outcomes and Treatments
- Renal and Vascular Pathologies
- Body Composition Measurement Techniques
- Trace Elements in Health
- Tissue Engineering and Regenerative Medicine
- Telomeres, Telomerase, and Senescence
- Neuroscience and Neural Engineering
- Organ Donation and Transplantation
Meyer Children's Hospital
2024
University of Florence
2024
Institut d'Investigació Biomédica de Bellvitge
2017-2024
Hospital Universitario Virgen del Rocío
2024
Universitat de Barcelona
2004-2022
Bellvitge University Hospital
2004-2022
Universidad de Alcalá
2022
Instituto de Salud Carlos III
2022
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2010-2020
Ajuntament de L’Hospitalet
2018-2020
Abstract Fibrogenesis is part of a normal protective response to tissue injury that can become irreversible and progressive, leading fatal diseases. Senescent cells are main driver fibrotic diseases through their secretome, known as senescence-associated secretory phenotype (SASP). Here, we report cellular senescence, multiple types in mice humans characterized by the accumulation iron. We show vascular hemolytic injuries efficient triggering iron accumulation, which turn cause senescence...
Macrophage infiltration is a common feature of the early phase renal ischaemia/reperfusion injury. Indeed, it generally regarded as cause tissue injury in this phase, although also clear that can lead to repair other phases. In order ascertain whether macrophages are directly involved repair/late which follows pro-inflammatory and process ischaemia/reperfusion, we used two different approaches based on macrophage depletion. Firstly, produced ischaemia mice were previously treated with...
To assess the immediate and long‐term effects of ischemic preconditioning (IPC) in deceased donor. liver transplantation (LT), we designed a prospective, randomized controlled trial involving 60 donors: control group (CTL, n = 30) or study (IPC, 30). IPC was induced by 10‐min hiliar clamping immediately before recovery organs. Clinical data blood samples were obtained donor recipient for measurements. significantly improved biochemical markers cell function such as uric acid, hyaluronic acid...
Tumor cell-derived factors, such as interleukin 10 (IL-10), polarize macrophages toward a regulatory M2 phenotype, characterized by the expression of anti-inflammatory cytokines and protumorigenic mediators.Here we explored molecular mechanisms allowing IL-10 to upregulate protein NGAL in primary human macrophages.Reporter assays full-length or deletion constructs promoter provided evidence that production is STAT3 dependent, activated downstream -Janus kinase (Jak) axis, well being C/EBP...
Abstract Inflammatory reactions are initiated to eliminate pathogens, but also promote repair of damaged tissue after acute inflammation is terminated. In this regard, macrophages play a prominent role during induction as well resolution and injury in various organs including the kidney. The present study describes mechanism for renal regeneration ischaemia/reperfusion injury. Following injury, apoptotic cell‐derived sphingosine‐1‐phosphate (S1P) or exogenously administered sphingosine...
Objective To define the protective effect of ischemic preconditioning on cold ischemia and reperfusion injury associated with intestinal transplantation, role nitric oxide in this process. Summary Background Data Ischemia/reperfusion continues to be a significant obstacle small bowel transplantation. Preconditioning is mechanism that protects against injury. Methods study capacity prevent ischemia-associated inflammatory response authors studied control group animals, groups or without...
In the case of living tissues, spectral width electrical bioimpedance dispersions (closely related with α parameter in Cole equation) evolves during ischemic periods. This is often ignored favor other parameters such as central frequency or resistivity at low frequencies. The object this paper to analyze significance through computer simulations (in and β dispersion regions) demonstrate its practical importance experimental studies performed rat kidneys cold preservation. indicate that could...
miRNA let-7e is involved in stem cell differentiation, and metalloproteinases are among its potential target genes. We hypothesized that the inhibitory action of on regulation MMP9 expression could represent a crucial mechanism during differentiation adipose-derived cells (ASCs). ASCs were differentiated with all-trans retinoic acid (ATRA) to promote effect let-7 silencing was tested. Results indicate cultured ATRA into epithelial lineage. found or transfected expressed markers such as...
Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation fibrosis in the unilateral ureteral obstruction (UUO) mice model.We stabilized by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) infused them into UUO mice. To ascertain were capable of reaching obstructed kidney, stained detected vivo tracking.We demonstrated that some reached kidney infusion...
Renal ischemia-reperfusion injury triggers an inflammatory response associated to infiltrating macrophages which determines the further outcome of disease. Brown Norway rats are known show endogenous resistance ischemia-induced renal damage. By contrast, Sprague Dawley exhibit a higher susceptibility ischemic injury. In order ascertain cytoprotective mechanisms, we focused on implication lipocalin-2 protein in main mechanisms ischemia/reperfusion by using adoptive macrophage administration,...
Previous studies have demonstrated that intestinal preconditioning protects the organ from ischemia reperfusion damage.Xanthine oxidase mediating free radical generation contributes to development of injury associated reperfusion. Thus, any process able modulate oxygen system could attenuate injury. Also, it is known nitric oxide implicated in response. The aim this work determine: (1) effect on xanthine system, (2) relevance injury, and (3) its relationship with oxide. For purpose, we...
This study investigated whether the renal regeneration occurring in recovery phase of kidney ischemia-reperfusion (I/R) is mediated by endogenously generated lipocalin-2 (Lcn2). A second objective was to examine Lcn2-mediated cell effects could be regulated inflammatory cytokines environment through their action on Lcn2 receptors (Lcn2R and megalin). Male Swiss mice were subjected 30 min ischemia with a reperfusion period 24 h (early reperfusion, expected time for maximum inflammation) 96...
This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. specific modulates Wnt pathway expression of early nephrogenic markers under these differentiation conditions. The WT1, Pax2 Wnt4 were downregulated when let-7e was silenced, thus indicating role in process. PKCβ, GSK3β phosphorylation (GSK3β(P)) β-catenin reduced differentiated reversed by silencing. Addition a PKCβ inhibitor to silenced abolished...
Abstract Alternatively activated macrophages (M2) have regenerative properties and shown promise as cell therapy in chronic kidney disease. However, M2 plasticity is one of the major hurdles to overcome. Our previous studies showed that genetically modified stabilized by neutrophil gelatinase‐associated lipocalin ( NGAL ) were able preserve their phenotype. Nowadays, little known about macrophage effects diabetic disease DKD ). The aim study was investigate therapeutic effect both bone...
Macrophage are specialized cells that contributes to the removal of detrimental contents via phagocytosis. Lipid accumulation in macrophages, whether from phagocytosis dying or circulating oxidized low-density lipoproteins, alters macrophage biology and functionality. It is known carnitine palmitoyl transferase 1-a (CPT1a) gene encodes an enzyme involved fatty acid oxidation and, therefore, lipid content. However, potential CPT1a activate phagocytic function have not been elucidated.Using a...
At present, Lupus Nephritis (LN) is still awaiting a biomarker to better monitor disease activity, guide clinical treatment, and predict patient's long-term outcome. In the last decade, novel biomarkers have been identified disease, but none incorporated into practice. The transmembrane receptor neuropilin-1 (NRP-1) highly expressed by mesangial cells its genetic deletion results in proteinuric glomerulosclerosis. NRP-1 increased kidney biopsies of LN. this work we were interested...
During the course of sepsis in critically ill patients, kidney dysfunction and damage are among first events a complex scenario toward multi-organ failure patient death. Acute injury triggers release lipocalin-2 (Lcn-2), which is involved both renal recovery. Taking into account that Lcn-2 binds transports iron with high affinity, we aimed at clarifying if fulfills different biological functions according to its iron-loading status cellular source during sepsis-induced failure. We assessed...