A5026727116- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Neuroscience and Neuropharmacology Research
- Conducting polymers and applications
- Parkinson's Disease Mechanisms and Treatments
- Neuroscience and Neural Engineering
- Neurotransmitter Receptor Influence on Behavior
- Ion channel regulation and function
- Neurological disorders and treatments
- Nuclear Receptors and Signaling
- Microfluidic and Capillary Electrophoresis Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Spectroscopy and Chemometric Analyses
- Photoreceptor and optogenetics research
- Tryptophan and brain disorders
- Advanced Chemical Sensor Technologies
- Heavy Metal Exposure and Toxicity
- Edible Oils Quality and Analysis
- Antibiotics Pharmacokinetics and Efficacy
- Trace Elements in Health
- Pharmacological Effects and Toxicity Studies
- Anesthesia and Neurotoxicity Research
University of Sassari
2015-2024
Institute of Sciences of Food Production
2016-2023
National Research Council
2010-2023
Escola Superior de Saúde Egas Moniz
2023
Champalimaud Foundation
2023
University of Lisbon
2023
University of Cagliari
2020
Brain Physiology Lab
2017
University College Dublin
2005-2013
National University of Ireland, Maynooth
2009-2012
A new telemetry system for simultaneous detection of extracellular brain glucose and lactate motion is presented. The device consists dual-channel, single-supply miniature potentiostat–I/V converter, a microcontroller unit, signal transmitter, miniaturized microvibration sensor. Although based on simple inexpensive components, the biotelemetry has been used accurate transduction anodic oxidation currents generated surface implanted biosensors animal microvibrations. was characterized...
The leucine-rich repeat kinase 2 (LRRK2) gene was found to play a role in the pathogenesis of both familial and sporadic Parkinson’s disease (PD). LRRK2 encodes large multi-domain protein that is expressed different tissues. To date, physiological pathological functions are not clearly defined. In this study we have explored controlling vesicle trafficking cellular or animal models using various readouts. neuronal cells, presence LRRK2G2019S mutant determines increased extracellular dopamine...
The activity of the noradrenergic system locus coeruleus (LC) is high in wake and low sleep. LC promotes arousal EEG activation, as well attention, working memory, cognitive flexibility. These functions rely on prefrontal cortex are impaired by sleep deprivation, but extent to which changes during remains unclear. Moreover, it unknown whether neurons can sustain elevated firing extended wake. Recent studies show that relative targeting primary motor (M1), those projecting medial (mPFC) have...
Adipose-derived stem cells (ADSCs) represent one of the cellular populations resident in adipose tissue. They can be recruited under certain stimuli and committed to become preadipocytes, then mature adipocytes. Controlling cell differentiation towards adipogenic phenotype could have a great impact on future drug development aimed at counteracting fat depots. Stem commitment influenced by different molecules, such as melatonin, which we previously shown an osteogenic inducer. Here,...
The β-lactam antibiotic ceftriaxone was suggested as a therapeutic agent in several neurodegenerative disorders, either for its ability to counteract glutamate-mediated toxicity, cerebral ischemia, or enhance the degradation of misfolded proteins, Alexander's disease. Recently, efficacy neuroprotection dopaminergic neurons rat model Parkinson's disease documented. However, which characteristics mediate effects remains unclear. Since, at molecular level, neuronal α-synuclein inclusions and...
By identifying endogenous molecules in brain extracellular fluid metabolomics can provide insight into the regulatory mechanisms and functions of sleep. Here we studied how cortical metabolome changes during sleep, sleep deprivation spontaneous wakefulness. Mice were implanted with electrodes for chronic sleep/wake recording microdialysis probes targeting prefrontal primary motor cortex. Metabolites measured using ultra performance liquid chromatography-high resolution mass spectrometry....
Abstract With the advancement of artificial intelligence(AI), platforms like ChatGPT have gained traction in different fields, including Medicine. This study aims to evaluate potential addressing questions related HIV prevention and assess its accuracy, completeness, inclusivity. A team consisting 15 physicians, six members from communities, three experts gender queer studies designed an assessment ChatGPT. Queries were categorized into five thematic groups: general information, behaviors...
Abstract : L‐DOPA and manganese both induce oxidative stress‐mediated apoptosis in catecholaminergic PC12 cells. In this study, exposure of cells to 0.2 m M MnCl 2 or 10‐20 μ neither affected cell viability, determined by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, nor induced apoptosis, tested flow cytometry, fluorescence microscopy, TUNEL technique. (50 ) decreases viability apoptosis. When was associated with 10, 20, 50 L‐DOPA, a concentration‐dependent...
Biosensors for glutamate (Glu) were fabricated from Teflon-coated Pt wire (cylinders and disks), modified with the enzyme oxidase (GluOx) electrosynthesized polymer PPD, poly(o-phenylenediamine). The polymer/enzyme layer was deposited in two configurations: before (GluOx/PPD) after (PPD/GluOx). These four biosensor designs characterized terms of response time, limit detection, Michaelis-Menten parameters Glu (J max K(M)(Glu)), sensitivity to linear region, dependence on oxygen concentration,...
Swiss mice were given 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 25 mg/kg/day, for 5 consecutive days and killed at different after MPTP discontinuance. Decreases in striatal tyrosine hydroxylase activity levels of dopamine its metabolites observed 1 day Ascorbic acid glutamate had increased, dehydroascorbic GSH decreased, whereas catabolites high-energy phosphates (inosine, hypoxanthine, xanthine, uric acid) unchanged. In addition, gliosis was both striatum substantia nigra...
Reports that globular proteins could enhance the interference blocking ability ofthe PPD (poly(o-phenylenediamine) layer used as a permselective barrier in biosensordesign, prompted this study where variety of modifying agents were incorporated into PPDduring its electrosynthesis on Pt-Ir electrodes. Trapped molecules, including fibrous proteinsand β-cyclodextrin, altered polymer/modifier composite selectivity by affecting thesensitivity to both H2O2 (signal molecule many enzyme-based...
A miniaturized biotelemetric device for the amperometric detection of brain tissue oxygen is presented. The new system, derived from a previous design, has been coupled with carbon microsensor real-time dissolved O2 in striatum freely moving rats. implantable consists single-supply sensor driver, current-to-voltage converter, microcontroller, and data transmitter. current converted to digital value by means an analog-to-digital converter integrated peripheral interface controller (PIC). sent...
Current evidence suggests a role of neuroinflammation in the pathogenesis Parkinson's disease (PD) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model basal ganglia injury. Reportedly, nonsteroidal anti-inflammatory drugs (NSAIDs) mitigate DAergic neurotoxicity rodent models PD. Consistent with these findings, epidemiological analysis indicated that certain NSAIDs may prevent or delay progression However, serious impediment chronic NSAID therapy, particularly elderly, is gastric,...