Wound-healing is a dynamic skin reparative process that results in sequence of events, including inflammation, proliferation, and migration different cell types as fibroblasts.Fibroblasts play crucial role repairing processes, from the late inflammatory phase until fully final epithelization injured tissue.Within this context, identifying tools able to implement proliferation could improve tissue regeneration.Recently, plants species all over world are coming out novel for therapeutic...

Human adipose-derived stem cells (hASCs) have been recently proposed as a suitable tool for regenerative therapies their simple isolation procedure and high proliferative capability in culture. Although hASCs can be committed into different lineages vitro, the differentiation is low-yield often incomplete process. We developed novel nonenzymatic method device, named Lipogems, to obtain fat tissue derivative highly enriched pericytes/mesenchymal by mild mechanical forces from human...

Somatic cells can be directly reprogrammed to alternative differentiated fates without first becoming stem/progenitor cells. Nevertheless, the initial need for viral-mediated gene delivery renders this strategy unsafe in humans. Here, we provide evidence that exposure of human skin fibroblasts a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering radio electric conveyed fields at radiofrequency 2.4 GHz, afforded remarkable commitment toward cardiac, neuronal, and...

Adipose-derived stem cells (ADSCs) represent one of the cellular populations resident in adipose tissue. They can be recruited under certain stimuli and committed to become preadipocytes, then mature adipocytes. Controlling cell differentiation towards adipogenic phenotype could have a great impact on future drug development aimed at counteracting fat depots. Stem commitment influenced by different molecules, such as melatonin, which we previously shown an osteogenic inducer. Here,...

Grape leaves influence several biological activities in the cardiovascular system, acting as antioxidants. In this study, we aimed at evaluating effect of ethanolic and water extracts from grape grown Algeria, obtained by accelerator solvent extraction (ASE), on cell proliferation. The amount total phenols was determined using modified Folin-Ciocalteu method, antioxidant were evaluated 2,2-diphenyl-l-picrylhydrazyl free radical (DPPH*) method ·OH scavenging electron paramagnetic resonance...

Radiofrequency (RF) waves from Wi-Fi (wireless fidelity) technologies have become ubiquitous, with Internet access spreading into homes, and public areas. The human body harbors multipotent stem cells various grading of potentiality. Whether may be affected by RF energy remains unknown. We exposed mouse embryonic (ES) to a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering 2.4 GHz its conveyer electrodes immersed the culture medium. Cell responses were investigated...

Abstract Decline in the gene expression of senescence repressor Bmi1 and telomerase, together with telomere shortening, underlay stem cells cultured for multiple passages. Here, we investigated whether impairment preventing mechanisms can be efficiently counteracted by exposure human adipose-derived to radio electric asymmetrically conveyed fields an innovative technology, named Radio Electric Asymmetric Conveyer (REAC). Due REAC exposure, number positively stained associated β-galactosidase...

Research for the use of physical means, in order to induce cell differentiation new therapeutic strategies, is one most interesting challenges field regenerative medicine, and then treatment neurodegenerative diseases, Parkinson's disease (PD) included. The aim this work verify effect radio electric asymmetric conveyer (REAC) technology on PC12 rat adrenal pheochromocytoma line, as they display metabolic features PD. cells were cultured with a REAC tissue optimization (TO-RGN) period ranging...

Abstract Hyaluronic acid (HA) plays a fundamental role in cell polarity and hydrodynamic processes, affording significant modulation of proliferation, migration, morphogenesis senescence, with deep implication the ability stem cells to execute their differentiating plans. The Radio Electric Asymmetric Conveyer (REAC) technology is aimed optimize ions fluxes at molecular level order mechanisms driving cellular asymmetry polarization. Here, we show that treatment 4-methylumbelliferone (4-MU),...

Inflammatory response represents one of the main mechanisms healing and tissue function restoration. On other hand, chronic inflammation leads to excessive secretion pro-inflammatory cytokines involved in onset several diseases. Oxidative stress condition may contribute worsening inflammatory state fall, increasing reactive oxygen species (ROS) production release. Polyphenols can counteract oxidative stress, modulating release toxic molecules interacting with physiological defenses, such as...

Background Development of molecules chemically modifying the expression crucial orchestrator(s) stem cell commitment may have significant biomedical impact. We recently developed hyaluronan mixed esters butyric and retinoic acids (HBR), turning cardiovascular fate into a high-yield process. The HBR mechanism(s) remain still largely undefined. Methodology/Principal Findings show that in both mouse embryonic (ES) cells human mesenchymal from fetal membranes term placenta (FMhMSCs),...

Human dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as conductor osteogenesis vitro vivo . Here, we attempted prime endogenous VEGF expression without need viral vector mediated gene transfer technologies. We show that hDPSCs exposure mixture hyaluronic, butyric,...

Human breast adenocarcinoma is a form of cancer which has the tendency to metastasize other tissues, including bones, lungs, brain, and liver. Several chemotherapeutic drugs are used treat tumors. Their combination simultaneously target different mechanisms involved in cell replication. Radio electric asymmetric conveyer (REAC) technology an innovative technology, both vitro vivo, induce reprogramming counteract senescence processes. Within this context, we treated MCF-7 cells with...

Melatonin, that regulates many physiological processes including circadian rhythms, is a molecule able to promote osteoblasts maturation in vitro and prevent bone loss vivo, while regulating also adipocytes metabolism. In this regard, we have previously shown melatonin combination with vitamin D, counteract the appearance of an adipogenic phenotype adipose derived stem cells (ADSCs), cultured favoring condition. present study, aimed at evaluating specific elicited by D based medium,...

Nutraceuticals present in food are molecules able to exert biological activity for the prevention and treatment of various diseases, form pharmaceutical preparations, such as capsules, cream, or pills. Myrtus communis L. is a spontaneous Mediterranean evergreen shrub, widely known liqueur obtained from its berries rich phytochemicals tannins flavonoids. In study, we aimed evaluate properties myrtle byproducts, residual industrial processing, Adipose-derived stem cells (ADSCs) induced at...

About 30 percent of patients diagnosed with myelodysplastic syndromes (MDS) progress to acute myeloid leukemia (AML). The senescence bone marrow?derived mesenchymal stem cells (BMSCs) seems be one the determining factors in inducing this drift. Research is continuously looking for new methodologies and technologies that can use bioelectric signals act on cell differentiation towards phenotype interest. Radio Electric Asymmetric Conveyer (REAC) technology, aimed at reorganizing endogenous...

Extracorporeal shock waves (ESWTs) are “mechanical” waves, widely used in regenerative medicine, including soft tissue wound repair. Although already being the clinical practice, mechanism of action underlying their biological activities is still not fully understood. In present paper we tried to elucidate whether a proinflammatory effect may contribute potential treatment. For this purpose, exposed human foreskin fibroblasts (HFF1 cells) an ESWT treatment (100 pulses using energy flux...

According to the WHO, overall age-standardized cancer rate keeps declining, and number of cases diagnosed each year increases, remaining among leading causes death in 91 out 172 recorded countries. In this context, novel prediction therapeutic protocols are compulsory. The effect a Stachys circinata L'Hér dichloromethane extract (ScDME) on cell redox homeostasis tumor proliferation was investigated. HepG2 feedback mechanisms oxidative stress exposure were evaluated by determining catalase...

It is currently known that pluripotent stem cells can be committed in vitro to the cardiac lineage by modulation of specific signaling pathways, but it also well that, despite significant increase cardiomyocyte yield provided available conditioned media, resulting cardiogenic commitment remains a highly variable process. Previous studies evidence radio electric fields asymmetrically conveyed through Radio Electric Asymmetric Conveyer (REAC) technology are able commit R1 embryonic and human...

Alterations of the gravitational environment are likely to modify cell behavior. Several studies have proven that T cells sensitive gravity alterations and microgravity conditions may induce immunosuppression weakened immune response in humans during spaceflights. The aim this work was elucidate if a specific treatment Radio Electric Asymmetric Conveyer (REAC) technology could restore, after mitogenic activation (Con A), correct expression cytokine IL2 gene its receptor IL2R alpha, which...