A5014696278- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Veterinary Equine Medical Research
- Cardiac Valve Diseases and Treatments
- Genetic and phenotypic traits in livestock
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Coronary Interventions and Diagnostics
- Reproductive Biology and Fertility
- TGF-β signaling in diseases
- Mast cells and histamine
- Infective Endocarditis Diagnosis and Management
- Cell death mechanisms and regulation
- Protease and Inhibitor Mechanisms
- Milk Quality and Mastitis in Dairy Cows
- Cardiac Imaging and Diagnostics
- Atrial Fibrillation Management and Outcomes
- Aortic Disease and Treatment Approaches
- Animal health and immunology
- Immune Response and Inflammation
- Animal Genetics and Reproduction
- Estrogen and related hormone effects
- Effects of Environmental Stressors on Livestock
- Angiogenesis and VEGF in Cancer
Institute of Animal Reproduction and Food Research
2015-2024
Polish Academy of Sciences
2013-2024
Józef Piłsudski University of Physical Education in Warsaw
2023
American Heart of Poland
2020-2023
Andrzej Frycz Modrzewski Krakow University
2022
Hebrew University of Jerusalem
2014
Agricultural Research Organization
2014
Okayama University
2013
University of Warmia and Mazury in Olsztyn
2013
Contents The bovine corpus luteum ( CL ) is a transient gland with life span of only 18 days in the cyclic cow. Mechanisms controlling development and secretory function may involve factors produced both within outside this gland. Although luteinizing hormone LH surge main trigger ovulation granulosa cells luteinization, many locally agents such as arachidonic acid AA metabolites, growth cytokines were shown to complement gonadotropins action process development. Bovine highly vascular...
The human endometrium is a fertility-determining tissue and target of steroid hormones' action. Endocrine disruptors (EDs) can exert adverse effects on the physiological function decidua at maternal–fetal interface. We examined potential an ED, bisphenol A (BPA), endometrial maturation/decidualization, receptivity, secretion decidual factors (biomarkers). In vitro decidualized, stromal cells from six hysterectomy specimens were treated with 1 pM–1 μM BPA, for 24 h assessed cell viability...
The aim of the study was to characterize endometrial mRNA transcription, immunolocalization, and protein expression interleukin (IL) 1alpha, IL1beta, IL6, IL1RI, IL1RII, IL6Ralpha/beta in course endometrosis during estrous cycle. Additionally, influence IL1alpha, IL6 on prostaglandin (PG) secretion PG synthase transcription tissue investigated. samples were obtained at early (n = 12), mid- late 12) luteal phases follicular phase Within each these phases, there four within category I, II, III...
We hypothesized that cytokines influence luteal angiogenesis in mares, while angiogenic factors themselves can also regulate secretory capacity. Therefore, the purpose of this study was to evaluate role cytokines—tumor necrosis factor alpha (TNF), interferon gamma (IFNG) and Fas ligand (FASL)—on vitro modulation activity mRNA level vascular endothelial growth A (VEGF), its receptor VEGFR2, thrombospondin 1 (TSP1), CD36 equine corpus luteum (CL) throughout phase. After treatment, VEGF protein...
Abstract Information on the prevalence of subclinical endometritis and its mechanism in repeat breeding cows is very limited. The aims this study were: a) to evaluate incidence disorder with cytobrush cytology b) analyze mRNA expression tumor necrosis factor α (TNFα) inducible nitric oxide synthase (iNOS) endometrial biopsy samples collected from without endometritis. Two experiments were carried out. In experiment 1, 112 (12.4%) (inseminated at least 3 times not pregnant) selected out 902 8...
Abstract Programmed necrosis (necroptosis) is an alternative form of programmed cell death that regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent, but a caspase (CASP)-independent pathway. In the present study, to determine if necroptosis participates in bovine structural luteolysis, we investigated RIPK1 RIPK3 expression throughout estrous cycle, during prostaglandin F2α (PGF)-induced luteolysis corpus luteum (CL), cultured luteal steroidogenic cells (LSCs) after...
Mare endometrial fibrosis (endometrosis), is one of the main causes equine infertility. Despite high prevalence, both ethology, pathogenesis and nature its progression remain poorly understood. Recent studies have shown that microRNAs (miRNAs) are important regulators in multiple cellular processes functions under physiological pathological circumstances. In this article, we reported changes miRNA expression at different stages endometrosis effect transforming growth factor (TGF)-β1 on most...
In the current study, transcriptome profiles of mare endometrium, classified into categories I, IIA, and IIB according to Kenney Doig, were compared using RNA sequencing, analyzed, functionally annotated in silico analysis. mild stage (IIA) endometrosis category I differentially expressed genes (DEGs) inflammation, abnormal metabolism, wound healing, quantity connective tissue. moderate (IIB) DEGs fibrosis, cellular homeostasis, mitochondrial dysfunction, pregnancy disorders. Ingenuity...
Proapoptotic factor Fas ligand (FASL) and its cell surface receptor FAS are tumor necrosis superfamily members that trigger apoptosis in different types. However, their influence on luteal steroidogenesis is not clearly understood. The aim of the present work was to determine (i) presence cytokine FASL mare's corpus luteum (CL) throughout phase, as well (ii) alone, or together with cytokines alpha (TNF) interferon gamma (IFNG), equine production luteotrophic luteolytic factors, viability,...
We have shown that bacteria induce neutrophil extracellular traps (NETs) in mare endometrium. Besides killing pathogens, NETs may contribute for endometrosis (chronic endometrium fibrosis). Since elastase (ELA) is a component regulates fibrosis and prostaglandin (PG) output, the aim was to evaluate if inhibition of ELA would affect collagen 1 (COL1) transcription PGs secretion by explants, different estrous cycle phases. Follicular-FP (n = 8) mid luteal-MLP 7) phases explants were cultured...
Although proteases found in neutrophil extracellular traps (NETs) have antimicrobial properties, they also stimulate collagen type 1 (COL1) production by the mare endometrium, contributing for development of endometrosis. Cathepsin G (CAT), a protease present NETs, is inhibited specific inhibitors, such as Inhibitor I (INH; β-keto-phosphonic acid). Metallopeptidases (MMPs) are involved equilibrium matrix. The objective this study was to investigate effect CAT and INH (a selective inhibitor)...
Prostaglandin endoperoxide synthase-2 ( PTGS2 ), tumour necrosis factor-alpha-induced protein-6 TNFAIP6 pentraxin-3 PTX3 epidermal growth factor-like factors: amphiregulin AREG ) and epiregulin EREG are essential for successful ovulation. In this study, we compared the induction of these ovulatory genes in bovine granulosa cells (GCs) vivo (after LH surge) vitro (forskolin (FRS) treatment). These were markedly stimulated GCs isolated from cows 21 h after LH-surge. GCs, FRS induced a distinct...
Recently, we showed that leukotrienes (LTs) regulate ovarian cell function in vitro. The aim of this study was to examine the role LTs corpus luteum (CL) during both estrous cycle and early pregnancy vivo. mRNA expression LT receptors (BLT for LTB(4) CYSLT LTC(4)), 5-lipoxygenase (5-LO) CL tissue their localization ovary were studied pregnancy. Moreover, concentrations (LTB(4) C(4)) blood measured. 5-LO BLT increased on days 16-18 cycle, whereas level evaluated compared with LTC(4), which...
Endometrosis, a fibrotic disease of mare endometrium, impairs uterine function. Prostaglandins (PG), despite modulating reproductive physiological functions, may also cause local pathological collagen deposition (fibrogenesis). We have previously shown that neutrophil extracellular traps (NETs) favor endometrosis. The aim this study was to investigate the effect enzymes present in NETs on PGF2α-pathway activation. Kenney and Doig’s type I/IIA IIB/III endometria, from follicular phase (FLP)...