A5037633999- Fungal Infections and Studies
- Nail Diseases and Treatments
- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Salivary Gland Disorders and Functions
- Pleural and Pulmonary Diseases
- Marine Bivalve and Aquaculture Studies
- Aquaculture Nutrition and Growth
- Echinoderm biology and ecology
- Plant Disease Resistance and Genetics
- Glycosylation and Glycoproteins Research
- Amoebic Infections and Treatments
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Diagnosis and treatment of tuberculosis
- Wnt/β-catenin signaling in development and cancer
- Nerve injury and regeneration
- Protease and Inhibitor Mechanisms
- Occupational and environmental lung diseases
- Immune Response and Inflammation
- Pancreatic function and diabetes
- Plant Reproductive Biology
- Ocean Acidification Effects and Responses
- Pediatric health and respiratory diseases
Tokyo Metropolitan Police Hospital
2021-2023
Nihon University
2011-2023
The University of Sydney
2014
Robert Bosch (India)
2014
Kawasaki Municipal Hospital
2011
University of Occupational and Environmental Health Japan
2007
East Japan Railway (Japan)
1999-2007
Aichi Health Promotion Foundation
2007
JR Tokyo General Hospital
1999
Japan Science and Technology Agency
1999
ZnT-1 is a Cation Diffusion Facilitator (CDF) family protein, and present throughout the phylogenetic tree from bacteria to humans.
Abstract The proinflammatory cytokine interleukin 1β (IL-1β) induces prostaglandin E 2 (PGE ) production via upregulation of cyclooxygenase-2 (COX-2) expression in synovial fibroblasts. This effect IL-1β is involved osteoarthritis. We investigated MAPK signaling pathways IL-1β-induced COX-2 feline In the presence inhibitors, and PGE release were both attenuated. induced phosphorylation p38, JNK, MEK, ERK1/2. A JNK inhibitor prevented not only but also MEK ERK1/2 IL-1β-stimulated cells,...
We investigated the in vitro differentiation of canine bone marrow stromal cells (BMSCs) into voltage- and glutamate-responsive neuron-like cells. BMSCs were obtained from healthy beagle dogs. Canine incubated with basal medium for neurons containing recombinant human basic fibroblast growth factor (bFGF; 100 ng/ml). The viability bFGF-treated was assessed by a trypan blue exclusion assay, morphology monitored. Real-time RT-PCR performed to evaluate mRNA expression neuronal, neural stem cell...
Human single-chain urokinase-type plasminogen activator without an N-glycosylation site (scu-PA-Q302) was produced in the methylotrophic yeast, Pichia pastoris using shortened prepeptide sequence of a fungal aspartic proteinase, Mucor pusillus rennin (MPR). The level (u-PA) immunoreactive material YPM medium 0·47 mg/l; however, most secreted product had been processed to smaller polypeptides. N-terminal amino acid major species identical that low molecular weight two-chain u-PA. Some...
Nucleotide sequences of chitin synthase 1 (CHS1) gene were analysed for the phylogenetic relation between Trichophyton violaceum and T. rubrum, including two isolates raubitschekii one isolate rubrum var. nigricans. About 620-bp genomic DNA fragments CHS1 amplified from these dermatophytes by polymerase chain reaction (PCR) sequenced. The nucleotide showed more than 95% similarity species. analysis their revealed that was genetically distinct violaceum. specific restriction endonuclease site...
Bone marrow stromal cells (BMSCs) are considered as candidates for regenerative therapy and a useful model studying neuronal differentiation. The role of basic fibroblast growth factor (bFGF) in differentiation has been previously studied; however, the signaling pathway involved this process remains poorly understood. In study, we investigated bFGF-induced canine BMSCs. bFGF induced mRNA expression neuron marker, microtubule associated protein-2 (MAP2) neuron-like morphological change...
Acute kidney injury (AKI) is characterized by a sudden loss of renal function. Early recognition AKI, especially in critically ill patients, essential for adequate therapy. Currently, neutrophil gelatinase-associated lipocalin (NGAL) considered to be an effective biomarker AKI; however, the regulation its expression and function tubular cells remains unclear. In this study, we investigated NGAL IL-1β-treated Madin-Darby canine (MDCK) as model cells. IL-1β induced disturbance localization...
Animal mycosis, particularly deep is one of the most challenging conditions encountered by veterinarians. Pathogens causing mycotic infections in animals include fungi such as Cryptococcus neoformans, Candida spp., and Aspergillus spp. The antifungal drugs used for treatment mycoses well humans are polyenes azoles. However, sensitivity clinical isolates obtained from toward these has rarely been assayed. In this study, activities itraconazole voriconazole against C. A. fumigatus isolated...
We have fused a cDNA gene encoding mature human serum albumin (HSA) to several secretory leader-encoding sequences. The hybrid genes were cloned into an episomal vector under the control of yeast promoters and then introduced cells. GAL1 promoter in combination with either native HSA pre-sequence or modified gave highest production immunoreactive HSA, 90 mg/liter being reached shake flask culture. invertase pre-sequence, mating factor α1 prepro-sequence, directed accurate processing. In...
Phosphofructokinase-1 (EC:2.7.1.11, PFK-1) catalyzes the phosphorylation of fructose 6-phosphate to 1,6-bisphosphate using adenosine triphosphate and is a key regulatory enzyme glycolysis. Mammalian PFK-1 isozymes are composed three kinds subunits (PFK-M, -L, -P), with different properties. It has been suggested that proportion in organs based on organ energy metabolism. In this study, we analyzed activity subunit composition canine PFK-1. We found that, dogs, skeletal muscle only PFK-M,...
Most isolates of Cryptococcus neoformans (teleomorph: Filobasidiella neoformans) from human patients and environmental materials in Japan have been identified as serotype A mating type a by the seroagglutination test experiments. PCR method using α allele-specific primer STE12 gene serotype- type-specific primers STE20 for identification C. has developed. Using method, conserved strains clinical feline cryptococcosis were examined type. The results showed that all A, MATα, indicating...
HBV‐ and HCV‐ Infected Workers in the Japanese Workplace, Rie N arai , et al . Department of Environmental Health, University Occupational Health —Around three million are persistently infected with HBV or HCV. Though most them work various industries, little is known about actual conditions their workplaces. To clarify workplace workers hepatitis, kinds questionnaire surveys, answered by occupational health physicians were carried out. The rates recognized as hepatitis B C 0.82% 0.48%...
To investigate the in vitro differentiation of canine bone marrow stromal cells (BMSCs) into functional, mature neurons.Bone from 6 adult dogs.BMSCs were isolated and chemically induced to develop neurons. The morphology BMSCs during neuronal induction was monitored, immunocytochemical analyses for neuron markers performed after induction. Real-time PCR methods used evaluate mRNA expression levels neural stem or progenitor cells, neurons, ion channels, western blotting assess proteins before...
Bisphenol A (BPA), an endocrine-disrupting chemical, is widely used in the production of polycarbonate plastic and epoxy resins. This study analyzed BPA concentration rat milk, order to assess risk transfer offspring via milk. The rats ingested by oral administration or drinking water a bottle, milk samples were collected using automated experimental milker. water, food was LC/MS. In case obtained from injected with at 2, 4, 8, 24 h prior milking, concentrations 0.462 +/- 0.182 ppm, 0.138...
Polymeric immunoglobulin receptor (pIgR) plays an intrinsic role in protecting the intestinal tract from invading pathogens. In present study, we observed a decrease pIgR colon lysate mice with dextran sodium sulfate (DSS) colitis. A was detected both mRNA and protein levels. Histologic examinations revealed marked destruction of epithelial cells (IECs), only small number regenerating IECs expressed pIgR. These results suggest that due to IECs. Because activation toll-like 3 slows...
This report deals with three additional canine cases of sterile panniculitis treated by oral administration tacrolimus and prednisolone. The treatment was proved to be an affordable medical protocol for good efficacy without adverse effects.