A5066890643- Planetary Science and Exploration
- Astro and Planetary Science
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Genetic Neurodegenerative Diseases
- Stellar, planetary, and galactic studies
- Metabolism and Genetic Disorders
- Cellular Mechanics and Interactions
- Space Science and Extraterrestrial Life
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Erythrocyte Function and Pathophysiology
- Heat shock proteins research
- Space exploration and regulation
- Advanced biosensing and bioanalysis techniques
- Spaceflight effects on biology
- Fungal and yeast genetics research
- Mitochondrial Function and Pathology
- Bone health and osteoporosis research
- Dental Radiography and Imaging
- Carbohydrate Chemistry and Synthesis
- Muscle activation and electromyography studies
- Parasite Biology and Host Interactions
Tokyo University of Agriculture and Technology
2015-2025
Eneos (Japan)
2024
Teikyo University
2024
RELX Group (Netherlands)
2023-2024
Mie University Hospital
2024
Tokyo Denki University
2021-2023
The Japanese Society of Gastroenterological Surgery
2023
Hiroshima University Hospital
2023
Iwate Medical University
2023
Nagoya University
2002-2022
Myotonic dystrophy (DM; OMIM 160900, also known as dystrophia myotonica, myotonia atrophica and Steinert disease) is an autosomal dominant myotonic myopathy associated with abnormalities of other organs, including eyes, heart, endocrine system, central peripheral nervous systems, gastrointestinal bone, skin.1 The mutation underlying DM expansion unstable cytosine-thymine-guanine (CTG) trinucleotide repeat in the 3' untranslated region protein kinase (DMPK) gene chromosome 19q13.3.2-4 In...
Several human hereditary neuromuscular disease genes are associated with the expansion of CTG or CGG triplet repeats. The DNA syntheses triplets ranging from 17 to 180 and repeats 9 160 in length were studied vitro. Primer extensions using Klenow fragment polymerase I, modified T7 (Sequenase), β paused strongly at specific loci pausings abolished by heating 70°C. As duplex DNA, but not single-stranded was increased, magnitude increased. location pause sites determined distance between site...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA 2-thiouridine derivative in tRNAGlu is a positive determinant for aminoacylation by Escherichia coli glutamyl-tRNA synthetaseLee A. Sylvers, Kelley C. Rogers, Miho Shimizu, Eiko Ohtsuka, and Dieter SollCite this: Biochemistry 1993, 32, 15, 3836–3841Publication Date (Print):April 20, 1993Publication History Published online1 May 2002Published inissue 20 April...
Long CTG triplet repeats which are associated with several human hereditary neuromuscular disease genes stabilized in ColE1-derived plasmids Escherichia coli containing mutations the methyl-directed mismatch repair (mutS, mutL, or mutH). When (CTG)180 were grown for about 100 generations mutS, mutH strains, 60-85% of contained a full-length repeat, whereas parent strain only 20% repeat. The deletions occur insert, not DNA flanking While many products heterogeneous length, preferential...
To find novel probes for duplex DNA, we prepared four types of triplexes containing a homopurine‐homopyrimidine 15‐mer and examined their thermal stabilities ( T m values). The single strands used triplex formation were DNA having defined C‐T mixed sequence, its sugar‐modified analogs, namely 2′‐fluoro RNA, 2′‐ O ‐methyl RNA. RNA the RNA‐containing similar in enhanced at pH 6.1 and, amongst triplexes, was most stable 5.0. Furthermore, an experiment using 34‐mer suggested that RNA‐triplex...
We have found that a water-soluble alkaline-digested form of eggshell membrane (ASESM) can provide an extracellular matrix (ECM) environment for human dermal fibroblast cells (HDF) in vitro. Avian (ESM) has fibrous-meshwork structure and long been utilized as Chinese medicine recovery from burn injuries wounds Asian countries. Therefore, ESM is expected to excellent natural material biomedical use. However, such applications hampered by the insolubility proteins. used recently developed...
The properties of duplex CTG·CAG and CGG·CCG, which are involved in the etiology several hereditary neurodegenerative diseases, were investigated by a variety methods, including circularization kinetics, apparent helical repeat determination, polyacrylamide gel electrophoresis. bending moduli 1.13 × 10<sup>−19</sup> erg·cm for CTG 1.27 CGG, ∼40% less than random B-DNA. Also, persistence lengths triplet sequences ∼60% value However, torsional repeats 2.3 10.4 base pairs (bp)/turn 2.4 10.3...
Obesity and its associated disorders, such as non-alcoholic steatohepatitis, increase the risk of hepatocellular carcinoma. Branched-chain amino acids (BCAA), which improve protein malnutrition in patients with liver cirrhosis, reduce carcinoma these obesity. In present study, effects BCAA supplementation on spontaneous development hepatic premalignant lesions, foci cellular alteration, db/db obese mice were examined. Male given a basal diet containing 3.0% either or casein,...
Abstract L-R (left and right) symmetry breaking during embryogenesis the establishment of asymmetric body plan are key issues in developmental biology, but onset including handedness-determining gene locus still remains unknown. Using pure dextral (DD) sinistral (dd) strains pond snail Lymnaea stagnalis as well its F 2 through to 10 backcrossed lines, single handedness-determining-gene was mapped by genetic linkage analysis, BAC cloning chromosome walking. We have identified actin-related...
Step width is a parameter that determines the size of base support (BOS) during gait. Further, it related to control center mass (COM) and trunk movements gait speed. The current study aimed validate effect conditioning using combined step (narrow wide) speed (normal [4.5 km/h] slow [2.2 km/h]) on segmental head, thorax, pelvis with respect BOS. behavior 17 healthy adult participants were measured treadmill walking three-dimensional motion analysis system. If was narrow, whole body segment...
Aging is a high-risk factor for obstructive and fibrotic lung diseases. Fibrotic disease leading to decreased function characterized by interstitial remodeling tissue scarring (sclerosis), with destruction of alveoli excess deposition type I collagen, an extracellular matrix component secreted fibroblasts. Therefore, regulating transforming growth factor-β (TGF-β) as profibrotic signal essential suppress pulmonary fibrosis. In fibrosis, TGF-β signaling mediated Smad YAP/TAZ, TAZ linked the...
To elucidate the effects of enamel matrix derivative (EMD: Emdogain) on bone regeneration in rat femurs after drill-hole injury, defects were filled with either EMD or its carrier, PGA, as control. On postoperative days 4 to 28, dissected examined by means various morphological approaches. In both experimental groups, formation trabecular bone, which was immunostained for sialoproteins (BSP), had occurred medullary cavities at cylindrical Day 7 postoperatively. Cuboidal osteoblasts clearly...
Signs of aging in facial skin correlate with lifespan and chronic disease; however, the health has not been extensively studied. In healthy young skin, dermis forms a type III collagen-rich dermal papilla, where capillary vessels supply oxygen nutrients to basal epidermal cells. Chicken eggshell membranes (ESMs) have used as traditional medicines promote wound healing Asian countries for many years. Previously, we designed an experimental system which human fibroblasts (HDFs) were cultured...
DNA templates containing a modified base (O6-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th 61st codon of ras gene were synthesized and deoxynucleotide incorporation opposite lesions investigated. The replicated by Taq polymerase, recombinant rat polymerase β mouse α-primase complex. Sequence analysis products indicated selective nucleotide(s) base, depending on kind polymerase. This system is very useful obtain...
Obese people and diabetic patients are known to be high risk of colorectal cancer (CRC), suggesting need a new preclinical animal model, by which extensively study the diverse mechanisms, therapy prevention. The present aimed determine whether experimental obese mice produced monosodium glutamate (MSG) treatment susceptible azoxymethane (AOM)-induced colon tumorigenesis using early biomarkers, aberrant crypts foci (ACF) β-catenin-accumulated (BCACs), carcinogenesis. Male Crj:CD-1 (ICR)...
The processes of bone wound healing after maxillary molar extraction in ovariectomized aged rats were examined by means quantitative backscattered electron image analysis and energy-dispersive X-ray microanalysis. Six-month-old female either sham-operated or underwent bilateral ovariectomy (OVX), 60 days postoperatively, the first molars extracted. On post-extraction 7, 30, 60, dissected resin-embedded maxillae micromilled transverse direction through extracted alveolar sockets, new...
DMPK, the product of DM locus, is a member same family serine-threonine protein kinases as Rho-associated enzymes. In DM, membrane inclusions accumulate in lens fiber cells producing cataracts. Overexpression DMPK cultured epithelial led to apoptotic-like blebbing plasma and reorganization actin cytoskeleton. Enzymatically active was necessary for both effects; inactive mutant did not produce either effect. Active RhoA but constitutive GDP-state produced similar effects DMPK. The actions...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetailed study of sequence-specific DNA cleavage triplex-forming oligonucleotides linked to 1,10-phenanthrolineMiho Shimizu, Hideo Inoue, and Eiko OhtsukaCite this: Biochemistry 1994, 33, 2, 606–613Publication Date (Print):January 18, 1994Publication History Published online1 May 2002Published inissue 18 January 1994https://pubs.acs.org/doi/10.1021/bi00168a028https://doi.org/10.1021/bi00168a028research-articleACS PublicationsRequest reuse...