A5063131792- Advanced Semiconductor Detectors and Materials
- Lipid Membrane Structure and Behavior
- Infrared Target Detection Methodologies
- Nanopore and Nanochannel Transport Studies
- Ion-surface interactions and analysis
- Calibration and Measurement Techniques
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Semiconductor materials and devices
- Microbial infections and disease research
- Oral microbiology and periodontitis research
- Semiconductor Quantum Structures and Devices
- Force Microscopy Techniques and Applications
- Graphene research and applications
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Plant and Biological Electrophysiology Studies
- Advanced Optical Sensing Technologies
- Carbon Nanotubes in Composites
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Spectroscopy and Quantum Chemical Studies
Janelia Research Campus
2025
Howard Hughes Medical Institute
2025
National Institute of Biomedical Imaging and Bioengineering
2023-2025
National Institutes of Health
2023-2025
Lehigh University
2016-2020
The Nature Conservancy
2020
Boise State University
2013-2019
Micron (United States)
2016
University of Arkansas at Fayetteville
2006-2013
Loyola University Maryland
2009
This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on three-dimensional graphene foam bioscaffolds. Specifically, we establish both bare laminin-coated as a biocompatible platform for muscle cells identify that electrical coupling stimulates cell activity. Cell functionality is determined by expression myotube heavy chain protein Ca2+ fluorescence, respectively. Further, our data show application pulsed stimulus to initiates contraction...
The Gram-negative bacterium, Aggregatibacter actinomycetemcomitans, has been associated with localized aggressive periodontitis (LAP). In particular, highly leukotoxic strains of A. actinomycetemcomitans have more closely this disease, suggesting that LtxA is a key virulence factor for actinomycetemcomitans. secreted across both the inner and outer membranes via Type I secretion system, but also found to be enriched within membrane vesicles (OMVs), derived from bacterial membrane. We...
The authors review those characterization techniques that have played significant roles in the development of HgCdTe infrared detector technology. They focus on two specific devices achieved widespread application for detection LWIR (8-12 mu m) and VLWIR (12-20 spectral regions: simple n-type photoconductor P-on-n LPE heterojunction photodiode. device physics these detectors, relate performance to starting material properties processing parameters, describe most important had a role their...
The capability of growing long-wavelength infrared HgCdTe liquid-phase epitaxy P-on-n heterojunction films with state-of-the-art photodiode performance, excellent thickness uniformity (±10%), and cutoff wavelength (e.g., 10.5±0.1 μm) across 2.5 cm×4.0 cm wafers has been demonstrated. In addition, we have extended the region operation to wavelengths 18–19 μm at 80 K. Both measured carrier lifetime data show that n-type base layers are quality, 77 K lifetimes calculated Auger-1 limit for film...
We demonstrate that 3 keV ion beams, formed from the common noble gasses, He, Ne, Ar, Kr, and Xe, can controllably "sculpt" nanometer scale pores in silicon nitride films. Single control of structural dimensions nanopores be achieved with all species despite a very wide range sputtering yields surface energy depositions. Heavy ions shrink more efficiently make thinner than lighter ions. The dynamics nanopore closing is reported for each results are fitted to an adatom diffusion model...
Recognition of and binding to cholesterol on the host cell membrane is an initial step in mechanism numerous pathogens, including viruses, bacteria, bacterial toxins; however, a viable method inhibiting this interaction has not yet been uncovered. Here, we describe by which recognition amino acid consensus peptide interacts with inhibits activity cholesterol-binding leukotoxin (LtxA). Using series biophysical techniques, have shown that recognizes hydroxyl group nanomolar affinity does...
The Gram-negative bacterium Aggregatibacter actinomycetemcomitans, commonly associated with localized aggressive periodontitis (LAP), secretes an RTX (repeats-in-toxin) protein leukotoxin (LtxA) that targets human white blood cells, interaction is driven by its recognition of the lymphocyte function-associated antigen-1 (LFA-1) integrin. In this study, we report on inhibition LtxA-LFA-1 binding as antivirulence strategy to inhibit LtxA-mediated cytotoxicity. Specifically, designed and...
The large-scale conformation of DNA molecules plays a critical role in many basic elements cellular functionality and viability. By targeting the structural properties DNA, cancer drugs, such as anthracyclines, effectively inhibit tumor growth but can also produce dangerous side effects. To enhance development innovative medications, rapid screening changes to provide important insight into their mechanism interaction. In this study, we report circular from intercalation with ethidium...
Abstract Optical aberrations hinder fluorescence microscopy of thick samples, reducing image signal, contrast, and resolution. Here we introduce a deep learning-based strategy for aberration compensation, improving quality without slowing acquisition, applying additional dose, or introducing more optics into the imaging path. Our method (i) introduces synthetic to images acquired on shallow side stacks, making them resemble those deeper volume (ii) trains neural networks reverse effect these...
The pore‐forming toxin lysenin self‐assembles large and stable conductance channels in natural artificial lipid membranes. exhibit unique regulation capabilities, which open unexplored possibilities to control the transport of ions molecules through Our investigations demonstrate that positively charged polymers polyethyleneimine chitosan inhibit conducting properties inserted into planar preservation inhibitory effect following addition on either side supporting membrane suggests presence...
There has been significant progress during the past several years in photovoltaic (PV) HgCdTe technology for advanced long wavelength remote sensing applications. Useful cutoff wavelengths have extended to beyond 17.0 micrometer. Junction quality improved point that D* greater than 3 multiplied by 10<SUP>11</SUP> cm-(root)Hz/W can be achieved arrays at temperatures of 60 - 65 K. The atmospheric infrared sounder (AIRS) instrument, scheduled launch year 2000 as part NASA EOS Program, uses over...
Abstract Lysenin is a pore-forming toxin, which self-inserts open channels into sphingomyelin containing membranes and known to be voltage regulated. The mechanistic details of its gating mechanism, however, remains elusive despite much recent efforts. Here, we have employed novel combination experimental computational techniques examine model for gating, that based on the existence an “effective electric dipole” inspired by reported structures lysenin. We support this mechanism observations...
The concentration and distribution of chlorine incorporated into silicon substrates during reactive ion beam etching is investigated. Rutherford backscattering spectrometry Auger sputter profiling are employed to analyze the single crystal samples. Data presented for samples etched at various energies, current densities, angles incidence. effect temperature, dopant concentration, crystalline orientation also discussed. amount retained by substrate decreases sharply with increasing energy...