A5103074090- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Diseases and Immunity
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Drug Transport and Resistance Mechanisms
- Liver Disease and Transplantation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Hypoxia, and Metabolism
- Hepatitis C virus research
- Diabetes Treatment and Management
- Gallbladder and Bile Duct Disorders
- Endoplasmic Reticulum Stress and Disease
- Diet and metabolism studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Transplantation Techniques and Outcomes
- Cancer, Lipids, and Metabolism
- Diabetes and associated disorders
- Neuroendocrine Tumor Research Advances
- Pancreatitis Pathology and Treatment
- Systemic Sclerosis and Related Diseases
- Drug-Induced Hepatotoxicity and Protection
- Chronic Myeloid Leukemia Treatments
- Peptidase Inhibition and Analysis
Marche Polytechnic University
2010-2020
Ospedali Riuniti di Ancona
1998-2013
Krankenhaus Oberndorf
2011
Essen University Hospital
2009
Beth Israel Deaconess Medical Center
2009
Harvard University
2009
Sorbonne Université
2009
Inserm
2009
Johannes Gutenberg University Mainz
2009
Center for HIV and Hepatogastroenterology
2008
Abstract Background/Aims: High‐fat dietary intake and low physical activity lead to insulin resistance, nonalcoholic fatty liver disease (NAFLD) steatohepatitis (NASH). Recent studies have shown an effect of glucagon‐like peptide‐1 (GLP‐1) on hepatic glucose metabolism, although GLP‐1 receptors (GLP‐1r) not been found in human livers. The aim this study was investigate the presence GLP‐1r exenatide, a analogue, signalling. Methods: expression evaluated biopsies livers high‐fat diet‐treated...
Nonalcoholic fatty liver disease (NAFLD) may lead to hepatic fibrosis. Dietary habits affect gut microbiota composition, whereas endotoxins produced by Gram-negative bacteria stimulate fibrogenesis. However, the mechanisms of action and potential effect in are still unknown. Thus, we sought analyze whether interfere with Mice fed control (CTRL) or high-fat diet (HFD) were subjected either bile duct ligation (BDL) CCl4 treatment. Previously gut-sterilized mice transplantation oral gavage...
Oxidative stress is associated with liver fibrosis and hepatic stellate cell (HSC) activation in vivo. However, it remains controversial whether oxidative contributes to HSC either directly or through a paracrine stimulation by damaged hepatocytes. A medium containing products released from cells undergoing was obtained after incubation of hepatocytes (HCM/Fe) without (HCM) 0.1 mmol/L ferric nitrilotriacetate complex (FeNTA). Exposure HCM/Fe for 24 hours significantly increased the number...
Abstract: Background /Aims: Reactive oxygen species (ROS) induce HSCs activation, proliferation and collagen gene expression in vitro . Nitric oxide (NO) represents a reactive molecule that reacts with ROS, yielding peroxynitrite. We thus verified the effect of NO on ROS‐induced correlated iNOS ROS formation to activation early phase liver injury leading hepatic fibrosis vivo Methods/Results : were incubated iron ascorbate (FeAsc) , which induced production, ERK1/2 phosphorylation increased...
Ethanol induces liver fibrosis by several means that include, among others, the direct fibrogenic action of acetaldehyde on hepatic stellate cells (HSC). However mechanisms responsible for this effect are not well understood. In communication we investigated signal transduction pathways triggered leading to upregulation α2(I) collagen and fibronectin gene expression in human HSC. Run–on assays showed acetaldehyde–enhanced transcription these 2 genes as early hours, via de novo protein...
NAFLD is the most common liver disease worldwide but it potential evolution to NASH and eventually hepatocellular carcinoma (HCC), even in absence of cirrhosis, that makes such clinical importance. Aim: we aimed create a mouse model reproducing pathological spectrum investigate role possible co-factors promoting HCC. Methods: mice were treated with choline-deficient L-amino-acid-defined-diet (CDAA) or its control (CSAA diet) subjected low-dose i.p. injection CCl4 vehicle. Insulin resistance...
Cholangiocarcinoma is a strongly aggressive malignancy with very poor prognosis. Effective therapeutic strategies are lacking because molecular mechanisms regulating cholangiocarcinoma cell growth unknown. Furthermore, experimental in vivo animal models useful to study the pathophysiologic of malignant cholangiocytes lacking. Leptin, hormone caloric homeostasis, which increased obese patients, stimulates several cancers, such as hepatocellular carcinoma. The aim this was define if leptin...
Acetaldehyde is fibrogenic and induces the expression of type I collagen genes in hepatic stellate cells. Some these acetaldehyde-dependent events are mediated by H2O2 thus establish a direct connection between oxidative stress upregulation. We localized to −378 −183 region α2(I) (COL1A2) promoter an acetaldehyde-responsive element (AcRE) functional human cells (HHSCs) investigated molecular mechanisms whereby acetaldehyde stimulates modulates its transcriptional activity. Because AcRE...
<h3>Background and aims:</h3> Progression of chronic cholestatic disorders towards ductopenia results from the dysregulation cholangiocyte survival, with cell death by apoptosis prevailing over compensatory proliferation. Currently, no therapy is available to sustain survival in course those disorders. It was recently shown that cholangiocytes express glucagon-like peptide-1 receptor (GLP-1R); its activation enhanced proliferative reaction cholestasis. The GLP-1R selective agonist exendin-4...
Lung fibrosis is a severe condition resulting from several interstial lung diseases (ILD) with different etiologies. Current therapy of ILD, especially those associated connective tissue diseases, rather limited and new anti-fibrotic strategies are needed. In this study, we investigated the activity in vivo human mesenchymal stromal cells obtained whole umbilical cord (hUC-MSC). Adult immunocompetent C57BL/6 mice (n. = 8 for each experimental condition) were injected intravenously hUC-MSC...
Abstract Background Survival of hepatic stellate cells ( HSC s) is a hallmark liver fibrosis, while the induction apoptosis may induce recovery. Activated are resistant to many pro‐apoptotic stimuli. To this issue, role Endoplasmic Reticulum ER ) stress in promoting s and consequently fibrosis resolution still debated. Aim evaluate potential stress‐mediated HSCs Methods were incubated with agonists, tunicamycin or thapsigargin. In vivo , isolated from normal, bile duct‐ligated BDL...
The activation of the biliary stem-cell signaling pathway hairy and enhancer split 1/pancreatic duodenal homeobox-1 (Hes-1/PDX-1) in mature cholangiocytes determines cell proliferation. Neurogenin-3 (Ngn-3) is required for pancreas development ductal neogenesis. PDX-1-dependent Ngn-3 initiates differentiation program by inducing microRNA (miR)−7 expression. Here we investigated role on cholangiocyte Expression levels miR-7 isoforms were tested from normal cholestatic human livers. was...
Increased cholangiocyte growth is critical for the maintenance of biliary mass during liver injury by bile duct ligation (BDL). Circulating levels testosterone decline following castration and cholestasis. Cholangiocytes secrete sex hormones sustaining autocrine mechanisms. We tested hypothesis that an trophic factor stimulating growth. The expression androgen receptor (AR) was determined in sections, male cholangiocytes, cultures [normal rat intrahepatic (NRICC)]. Normal or BDL (immediately...