A5026222529- Lung Cancer Treatments and Mutations
- HER2/EGFR in Cancer Research
- Lung Cancer Research Studies
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Gastric Cancer Management and Outcomes
- Genetic factors in colorectal cancer
- Neuroendocrine Tumor Research Advances
- Breast Cancer Treatment Studies
- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- Salivary Gland Tumors Diagnosis and Treatment
- Adrenal and Paraganglionic Tumors
- Genomic variations and chromosomal abnormalities
- Colorectal and Anal Carcinomas
- Peptidase Inhibition and Analysis
- Radiomics and Machine Learning in Medical Imaging
- Cancer, Lipids, and Metabolism
- Lymphoma Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Hypoxia, and Metabolism
Peking Union Medical College Hospital
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Academy of Medical Sciences
2022
National Cancer Center
2020
Union Hospital
2014
Peking University
2007
Chinese PLA General Hospital
2007
Introduction Pulmonary mucoepidermoid carcinoma (PMEC) is an uncommon neoplasm of the lung and main salivary gland-type carcinoma. The aims this study were to review clinicopathological immunohistochemical features PMEC characterize genetic events in PMEC. Methods We reviewed pathology cases our hospital found 34 initially diagnosed cases, 26 which confirmed as after excluding 8 MEC-like pulmonary characteristics retrospectively reviewed. MAML2 rearrangement was detected by fluorescence In...
The EGFR and HER2 genes are located on chromosomes 7 17, respectively. They therapeutic targets in some tumors. TOP2A gene, which is near chromosome the target of many chemotherapeutic agents, co-amplification has been described several tumor types. Herein, we investigated gene status EGFR, HER2, Chinese gastric carcinoma patients. We determined rate polysomy for attempted to clarify relationship between copy number increased expression their encoded proteins. Furthermore, tried address...
Abstract Background This study was designed to investigate EGFR protein expression, copy number and mutations in lung adenocarcinomas, explore the relationship of three markers. Methods status analyzed surgically resected adenocarcinoma samples from 133 Chinese patients by methods: expression (n = 133) standardized immunohistochemistry (IHC), gene fluorescence situ hybridization (FISH), mutation analysis using Scorpion amplification refractory system (ARMS) 133). Results The results showed...
EML4-ALK is a new driver gene of non-small cell lung cancer and target crizotinib. The objectives this study were to determine the frequency ALK rearrangements in large cohort patients with primary adenocarcinoma analyze association clinicopathological characteristics clinical outcomes. roles fluorescence situ hybridization (FISH), Ventana immunohistochemistry (IHC), reverse transcriptase polymerase chain reaction (RT-PCR) detection evaluated. rearrangement was detected 430 specimens from...
Lung adenocarcinoma (AD) is a common variant of non-small cell lung cancer (NSCLC). Programmed death protein 1/programmed ligand 1 (PD1/PD-L1) are promising immunotherapy targets and its expression may be an important biomarker predicting clinical response. In this study, we evaluated PD-L1 in conjunction with clinicopathological characteristics outcomes resected adenocarcinoma.This study included 133 cases adenocarcinoma. rate was 16.5% at the mRNA level 13.5% level, kappa coefficient two...
Lung adenosquamous cell carcinomas (ASCs) is a rare variant of NSCLC with poorer prognosis and fewer treatment option than the more common variants. PD-L1 expression reported to be predictor clinical response in trials NSCLC. In our study, was evaluated via immunohistochemistry using specific monoclonal antibody (SP263), mRNA situ hybridization. This study included 51 ASCs, 133 lung adenocarcinomas, 83 squamous (SCC). Similar results were obtained for measured at protein level (k...
HER2/ERBB2 status is used to select patients for HER2-targeted therapy. amplification/overexpression of upper gastrointestinal (UGI) adenocarcinomas was determined locally or in two central laboratories the TRIO-013/LOGiC trial chemotherapy with without lapatinib. Patients selected had laboratory confirmation HER2 amplification inclusion primary efficacy population. assessed PathVysion IQ PharmDx FISH and HercepTest immunohistochemistry assays. Associations outcomes were retrospectively...
Background and Aim Poor response to immune checkpoint inhibitors (ICIs) has been observed in most triple-negative breast cancer (TNBC) cases (around 80%). Our aim was investigate the status of mismatch repair (MMR), microsatellite instability (MSI), programmed death-ligand 1 (PD-L1), lymphocyte-activation gene 3 (LAG-3) TNBC. Methods A total 74 TNBC samples were retrospectively analyzed. MMR MSI evaluated by immunohistochemistry (IHC) polymerase chain reaction (PCR) using Promega 1.2 NCI...
Fused information from protein status, DNA breakage, and transcripts are still limited because of the low rate activated-NTRK in colorectal cancer (CRC). In total, 104 archived CRC tissue samples with dMMR were analyzed using immunohistochemistry (IHC), polymerase chain reaction (PCR), pyrosequencing to mine NTRK-enriched group, then subjected NTRK fusion detection pan-tyrosine kinase IHC, fluorescence situ hybridization (FISH), DNA-/RNA-based next generation sequencing (NGS) assays. Of 15...
c-ros oncogene 1 (ROS1) rearrangement presents one of the newest molecular targets in non-small cell lung cancer (NSCLC). ROS1 is predominantly found adenocarcinoma cases and exclusive to other oncogenes, such as epidermal growth factor receptor (EGFR), Kirsten rat sarcoma viral homolog (KRAS), anaplastic lymphoma kinase (ALK). The aim this study was investigate clinicopathological characteristics outcomes ROS1-rearranged patients with without EGFR KRAS mutations ALK rearrangements.Wild-type...
Abstract Background Although microsatellite instability (MSI) is most commonly detected in colorectal cancer (CRC), improvement MSI analysis method can always help us better assessing phenotypes and gaining useful information challenging cases. The purpose of current study to explore whether the ProDx® System (ProDx® MSI) improve classification CRC. Methods We compared profiles 97 FFPE samples from CRC patients by with Promega 1.2 NCI panel. result then confirmed IHC test, which evaluate MMR...
The aim of this study was to describe the clinicopathological characteristics and high-risk human papillomavirus (HPV) infection status in patients diagnosed with co-existing adenoid cystic carcinoma (ACC) invasive squamous cell (SCC) uterine cervix. Three were identified from pathology databank Peking Union Medical College Hospital year 2000 2014. Immunohistochemistry situ hybridization (ISH) employed study. aged 64, 77 63 years (average, 68 old). All postmenopausal women who presented...
Abstract Objective A standardized procedure of fused REarranged during Transfection (RET) gene detection using fluorescence in situ hybridization (FISH) remains to be established papillary thyroid carcinoma (PTC). Our purpose was investigate false-negative and false-positive events their FISH signal characteristics. Methods total 111 PTC cases were analyzed break-apart probes for RET status evaluation. All results validated fusion-induced asymmetric transcription assay (FIATA)-based reverse...
The paradigm for combined small-cell lung carcinoma (C-SCLC) is according to standard SCLC treatment with poor outcomes. efficacy of immune checkpoint inhibitor (ICI) monotherapy pretreated still limited. Clinical researches exploring radiotherapy immunotherapy showed promising synergistic effects in several tumors. We report one C-SCLC case after resistant comprehensive receiving nivolumab achieving clinical complete remission (CCR). combination module ICI and radiation could be an option...
Non-Hodgkin's lymphoma (NHL) primarily derived from the base of tongue, is rare. Human papillomavirus (HPV) and Epstein-Barr virus (EBV) are important aetiological risk factors for tumours head neck. This study describes clinicopathological features NHL in tongue status HPV EBV these cases.Seven cases were identified Pathological Registry Database at Peking Union Medical College Hospital (PUMCH). The utilized immunochemistry, situ hybridization (ISH), gene rearrangement to confirm disease...
To our knowledge there are few reports on the K-ras mutation pattern of pancreatic adenocarcinoma from Chinese mainland patients. We examined surgically resected formalin-fixed, paraffin-embedded primary tissue blocks for presence activating point mutations at codon 12 and 13 gene.Mutations were detected through use microdissection, polymerase chain reaction (PCR) direct sequencing. The results confirmed by reverse sequencing.The combination PCR sequencing techniques resulted in a rapid...
Survivin has functions in both the regulation of cell division and inhibition apoptosis, it is expressed most human tumors but not normal adult tissues. It a potential target for cancer gene therapy. In present study, we designed DNAzyme targeting survivin mRNA tried to determine its effect on pancreatic carcinoma line PANC-1.We synthesized '10-23' antisurvivin DNAzyme, testified cleavage activity by cell-free test, then delivered into PANC-1 through liposome, detected expression reverse...
Rare NTRK-driven malignant neoplasms can be effectively inhibited by anti-TRK agents. The discovery of NTRK1/2/3-rich tumors in papillary thyroid cancer (PTC) patients is a precondition for the rapid identification NTRK fusion tumors. Knowledge gene activation critical to accurately detect status. A total 229 BRAF V600E-negative samples from PTC were analyzed this study. Break-apart fluorescence situ hybridization (FISH) was performed RET fusion. status using FISH, DNA- and RNA-based...