A5009875147- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Tendon Structure and Treatment
- Surgical Sutures and Adhesives
- Medical Imaging and Analysis
- Cervical and Thoracic Myelopathy
- Periodontal Regeneration and Treatments
- Silk-based biomaterials and applications
- Tissue Engineering and Regenerative Medicine
- Reconstructive Surgery and Microvascular Techniques
- Magnetic and Electromagnetic Effects
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- TGF-β signaling in diseases
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- Spaceflight effects on biology
- Spondyloarthritis Studies and Treatments
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Shoulder Injury and Treatment
- Congenital limb and hand anomalies
- Rheumatoid Arthritis Research and Therapies
- Hemostasis and retained surgical items
University of Bern
2016-2024
University Hospital of Bern
2024
Lucerne University of Applied Sciences and Arts
2017
CIC nanoGUNE
2013
Background Recently, Tie2/TEK receptor tyrosine kinase (Tie2 or syn. angiopoietin‐1 receptor) positive nucleus pulposus progenitor cells were detected in human, cattle, and mouse. These show remarkable multilineage differentiation capacity direct correlation with intervertebral disc (IVD) degeneration are therefore an interesting target for regenerative strategies. Nevertheless, there remains controversy over the presence function of these Tie2 + (NPCs), part due to difficulty identification...
(1) Background: Intervertebral disc (IVD) repair represents a major challenge. Using functionalised biomaterials such as silk combined with enforced hydrogels might be promising approach for repair. We aimed to test an IVD by combining genipin-enhanced fibrin hydrogel engineered scaffold under complex load, after inducing injury in bovine whole organ culture; (2) Methods: Bovine coccygeal IVDs were isolated from ~1-year-old animals within four hours post-mortem. Then, the annulus fibrosus...
For the example of peptides and proteins, we contrast "natural" self-assembly, i.e. aggregation in solutions, with "forced" assembly by electrospinning, application strong electrical fields to concentrated solutions. We were able spin fibres that contain short stretches diameters down 5 nm; ultimate aim is a fibre size single molecule. Besides their wide biochemical relevance, small can assemble defined supramolecular structures such as tubes. While main driving mechanism electrospinning...
Intervertebral disc (IVD) repair is a high-priority topic in our active and increasingly ageing society. Since high number of people are affected by low back pain treatment options that able to restore the biological function IVD highly warranted. Here, we investigated whether feasibility genetically engineered (GE)-silk from Bombyx mori containing specific growth factors precondition human bone-marrow derived mesenchymal stem cells (hMSC) or activate differentiated annulus fibrosus (hAFC)...
Low back pain (LBP) is an increasing global health problem associated with intervertebral disc (IVD) trauma and degeneration. Current treatment options include surgical interventions partial unsatisfactory outcomes reported such as failure to relieve LBP, nonunions, nerve injuries, or adjacent segment disease. Cell-based therapy tissue engineered IVD constructs supplemented transfected cells that incorporate factors enhancing matrix synthesis represent appealing approach regenerate the IVD....
Low back pain related to intervertebral disk (IVD) degeneration has a major socioeconomic impact on our aging society. Therefore, stem cell therapy activate self-repair of the IVD remains an exciting treatment strategy. In this respect, tissue-specific progenitors may play crucial role in regeneration, as these cells are perfectly adapted niche. Such rare progenitor population residing nucleus pulposus (NP) (NP [NPPCs]) was found positive for angiopoietin-1 receptor (Tie2+), and demonstrated...
Due to the poor self-healing capacities of anterior cruciate ligament, previous primary repair attempts have failed. To enhance biologic healing, platelet rich plasma and collagen scaffold shown promise in animal models. Platelet (PRP) is already used several clinical applications although outcomes are quite debated. The purpose this study was examine effects different PRP formulations during 21 days: With leucocytes pure on human ligament-derived ligamentocytes grown patches 3D cell...
Spinal fusion is a common surgical procedure to address range of spinal pathologies, like damaged or degenerated discs. After the removal intervertebral disc (IVD), structural spacer positioned followed by internal fixation, and segment natural bone growth. Due their osteoinductive properties, morphogenetic proteins (BMP) are applied promote fusion. Although successful in most patients, rates non-unions after lumbar spine from 5% 35%. Clinical observations recent studies indicate, that...
Spinal fusion surgery is a common treatment modality for various pathologic conditions of the spine. The bone morphogenetic protein 2 (BMP2) analogue L51P acts as general inhibitor BMP antagonists, whereas it shows weak affinity type I receptor. It suggested that applied in disorders might prevent side effects highly concentrated dosage applications order milligrams. objective this study was to investigate and BMP2 on intervertebral disc cells (IVDCs), i.e. nucleus pulposus cells, annulus...
Abstract The estimation of BMD with CT scans requires a calibration method, usually based on phantom. In asynchronous calibration, the phantom is scanned separately from patient. A standardized acquisition protocol must be used to avoid variations between patient and However, can still induced, for example, by temporal fluctuations or characteristics. Based further use 739 forensic 111 clinical scans, this study uses proximal femur value (“total hip”) assess accuracy, using in-scan as ground...
Introduction Discectomy and spinal fusion represents the gold standard treatment for surgery to relieve pain. Fusion can be hindered, however, yet unknown reasons that lead non-fusions with pseudo-arthrosis. We previously showed intervertebral disc (IVD)-derived cells hinder ossification process of human bone marrow-derived stromal (hMSC). 1 Within this study, we hypothesized BMP-antagonists secreted by IVD are factors responsible such inhibition reversed addition L51P. L51P is an engineered...
Introduction Almost 80% of the population suffers from low back pain at least once in their life causing a high socio economic burden. Often these pains originate trauma resulting disc herniation and /or degeneration. Despite this prevalence associated costs, satisfying treatment method is not yet on market. Here we aimed to develop new seal repair annulus fibrosus (AF) injuries by using genetically engineered silk carrying GDF-6 growth factor known lead stem cells toward an intervertebral...
It is not fully understood how cells detect external mechanical forces, but mechanosensitive ion channels play important roles in detecting and translating physical forces into biological responses (mechanotrans-duction). With the “OoClamp” device, we developed a tool to study electrophysiological processes, including gating properties of under various gravity conditions. The device uses an adapted patch clamp technique operational during parabolic flight centrifugation up 20 g. In framework...
Abstract It is not fully understood how cells detect external mechanical forces, but mechanosensitive ion channels play important roles in detecting and translating physical forces into biological responses (mechanotransduction). With the “OoClamp” device, we developed a tool to study electrophysiological processes, including gating properties of under various gravity conditions. The device uses an adapted patch clamp technique operational during parabolic flight centrifugation up 20 g . In...