A5084834995- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Systemic Lupus Erythematosus Research
- RNA Research and Splicing
- RNA modifications and cancer
- Vasculitis and related conditions
- Circular RNAs in diseases
- Glycosylation and Glycoproteins Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Sphingolipid Metabolism and Signaling
- CAR-T cell therapy research
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Toxin Mechanisms and Immunotoxins
- Cancer Immunotherapy and Biomarkers
- Renal Transplantation Outcomes and Treatments
- Galectins and Cancer Biology
- Phagocytosis and Immune Regulation
- Virus-based gene therapy research
University Medical Center Groningen
2015-2025
University of Groningen
2014-2025
Dialyse Centrum Groningen
2000-2018
Seoul National University
2014
Abstract In a previous study we demonstrated high expression of the non‐coding BIC gene in vast majority Hodgkin's lymphomas (HLs). Evidence suggesting that is primary microRNA transcript containing mature microRNA‐155 (miR‐155) as part RNA hairpin now accumulating. We therefore analysed HL cell lines and tissue samples to determine whether miR‐155 also expressed HL. High levels could be demonstrated, indicating processed into Most non‐HL subtypes were negative for determined by RNA‐ISH....
MicroRNAs (miRNAs) are a set of small, non-protein-coding RNAs that regulate gene expression at the post-transcriptional level. Maturation miRNAs comprises several regulated steps resulting in approximately 22-nucleotide single-stranded mature miRNAs. Regulation miRNA can occur both transcriptional level and during processing. Recent studies have elucidated specific aspects well-regulated nature processing involving various regulatory proteins, editing transcripts, cellular location. In...
Abstract In a search for genes specifically expressed in Reed–Sternberg (RS) cells of Hodgkin lymphoma (HL), we applied the serial analysis gene expression (SAGE) technique on HL‐derived cell line DEV. Genes highly DEV were subjected to an RT‐PCR confirm SAGE results. For one genes, high was observed and other lines but not non‐Hodgkin (NHL)–derived normal controls, suggesting HL‐specific expression. This corresponds human BIC gene, member noncoding mRNA‐like molecules. RNA situ...
Abstract We previously demonstrated high expression of primary‐microRNA BIC (pri‐miR‐155) in Hodgkin lymphoma (HL) and lack most non‐Hodgkin subtypes including some Burkitt (BL) cases. Recently, was reported BL comparison to pediatric leukemia normal peripheral‐blood samples. In this study, we extended our series cases cell lines examine using RNA situ hybridization (ISH) quantitative RT‐PCR (qRT‐PCR) miR‐155 Northern blotting. Both ISH qRT‐PCR revealed no or low levels 25 tissue samples...
Background Obesity promotes inflammation in adipose tissue (AT) and this is implicated pathophysiological complications such as insulin resistance, type 2 diabetes cardiovascular disease. Although based on the classical hypothesis, necrotic AT adipocytes (ATA) obese state activate macrophages (ATM) that then lead to a sustained chronic AT, link between human source of has not been in-depth systematically studied. So we decided new hypothesis investigate primary alone see whether they are...
Hodgkin lymphoma (HL) is derived from preapoptotic germinal center B cells, although a general loss of cell phenotype noted. Using quantitative reverse transcription-polymerase chain reaction and miRNA microarray, we determined the microRNA (miRNA) profile HL compared this with panel B-cell non-Hodgkin lymphomas. The two methods showed strong correlation for detection expression levels. HL-specific included miR-17-92 cluster members, miR-16, miR-21, miR-24, miR-155. large lines, found...
MicroRNA (miRNA) sponges are transcripts with repeated miRNA antisense sequences that can sequester miRNAs from endogenous targets. MiRNA valuable tools for loss-of-function studies both in vitro and vivo. We developed a fast flexible method to generate tested their efficiency various assays. Using single directional ligation reaction we generated 10 or more binding sites. Luciferase AGO2-immuno precipitation (IP) assays confirmed effective of the sponges. GFP competition assay showed miR-19...
The search for potential targets ceramide action led to the identification of ceramide-activated protein phosphatases (CAPP). To date, two serine/threonine phosphatases, phosphatase 2A (PP2A) and 1 (PP1), have been demonstrated function as phosphatases. In this study, we show that treatment with either anti-FAS IgM (CH-11) (150 ng/ml) or exogenous d-(e)-C(6-)ceramide (20 microm) induces dephosphorylation PP1 substrates, serine/arginine-rich (SR) proteins, in Jurkat acute leukemia T-cells....
B-cells, triggered via their surface B-cell receptor (BcR), start an apoptotic program known as activation-induced cell death (AICD), and it is widely believed that this phenomenon plays a role in the restriction focusing of immune response. Although both ceramide caspases have been proposed to be involved AICD, contribution either exact molecular events through which AICD commences are still unknown. Here we show Ramos BcR-triggered associated with early rise C16 derives from activation de...
The study of human microRNAs is seriously hampered by the lack proper tools allowing genome-wide identification miRNA targets. We performed Ribonucleoprotein ImmunoPrecipitation—gene Chip (RIP-Chip) using antibodies against wild-type Ago2 in untreated Hodgkin lymphoma (HL) cell lines. Ten to thirty percent gene transcripts from genome were enriched Ago2-IP fraction cells, representing HL miRNA-targetome. In silico analysis indicated that ∼40% these represent targets abundantly co-expressed...
In this study, we describe an ordered formation of long- and very long-chain ceramide species in relation to the progression B-cell receptor (BcR) triggering induced apoptosis. An early caspase-independent increase species, which C16- predominated, was observed 6 h after BcR triggering. contrast, were generated later, 12–24 The these C24-ceramide required activation effector caspases. BcR-induced resulted proteasomal degradation XIAP subsequent caspases, demonstrating important...
Immune cell-type specific miRNA expression patterns have been described but the detailed role of single miRNAs in function T-cells remains largely unknown. We investigated miR-21 primary human CD4+ T-cells. MiR-21 is substantially expressed with a memory phenotype, and robustly upregulated upon αCD3/CD28 activation both naive By inhibiting endogenous activated T-cells, we showed that regulates fundamentally different aspects T-cell biology, depending on differentiation status T-cell. Stable...
Elderly kidney transplant recipients (KTRs) represent almost one third of the total population. These patients have a very high coronavirus disease 2019 (COVID-19)-related mortality, whereas their response to COVID-19 vaccination is impaired. Finding ways improve in this vulnerable population uttermost importance.
BackgroundSystemic sclerosis-interstitial lung disease (SSc-ILD) is the leading cause of death in patients with SSc. There an unmet need for predictive biomarkers to identify SSc at risk ILD. Previous studies have shown that interferon (IFN) pathways may play a role We assessed use C-X-C motif chemokine ligand 10 (CXCL10) as biomarker new onset ILD SSc.MethodsOne-hundred-sixty-five (Female, N = 130) (SSc-ILD, 41) and 13 8) healthy controls were investigated retrospectively. CXCL10 protein...
Objective To evaluate serum neurofilament light chain (sNfL) as biomarker of disease onset, progression and treatment effect in hereditary transthyretin (ATTRv) amyloidosis patients TTR variant (TTRv) carriers.
Human papillomavirus (HPV) infection is the major cause of (pre)malignant cervical lesions. We previously demonstrated that Vvax001, a replication-incompetent Semliki Forest virus (SFV) vaccine encoding HPV type 16 (HPV16) E6 and E7, induced potent anti-E6 -E7 cytotoxic T-cell responses. Here, we investigated clinical efficacy Vvax001 in patients with HPV16-positive intraepithelial neoplasia grade 3 (CIN3). Patients newly diagnosed CIN3 were eligible for participation. received immunizations...