A5029177591- Fungal and yeast genetics research
- Cellular Mechanics and Interactions
- Plant and fungal interactions
- Heat shock proteins research
- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- Mollusks and Parasites Studies
- Cardiomyopathy and Myosin Studies
- Advanced Fluorescence Microscopy Techniques
- Algal biology and biofuel production
- Advanced Biosensing Techniques and Applications
- Plant Reproductive Biology
- Molecular Biology Techniques and Applications
- 14-3-3 protein interactions
- Fungal Biology and Applications
- bioluminescence and chemiluminescence research
- Fermentation and Sensory Analysis
- Microbial Metabolic Engineering and Bioproduction
- Advanced Proteomics Techniques and Applications
- Cancer Genomics and Diagnostics
Institut Fresnel
2020-2024
Centre National de la Recherche Scientifique
2015-2022
Centrale Marseille
2020-2022
Mosaic Company (United States)
2021-2022
Architecture et Fonction des Macromolécules Biologiques
2015
Aix-Marseille Université
2015
Institut de Biologie du Développement Marseille
2014
Septins are cytoskeletal proteins conserved from algae and protists to mammals. A unique feature of septins is their presence as heteromeric complexes that polymerize into filaments in solution on lipid membranes. Although animal associate extensively with actin-based structures cells, whether organize cells if septin organization impacts function not known. Customizing a tripartite split-GFP complementation assay, we show all decorating actin stress fibers octamer-containing filaments....
Septin GTP-binding proteins contribute essential biological functions that range from the establishment of cell polarity to animal tissue morphogenesis. Human septins in cells form hetero-octameric septin complexes containing ubiquitously expressed SEPT9 subunit (also known as SEPTIN9). Despite established role mammalian development and human pathophysiology, biochemical biophysical studies have relied on monomeric SEPT9, thus not recapitulating its native assembly into complexes. We a...
ABSTRACT Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, are thus important regulators cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors mutated hereditary neuralgic amyotrophy (HNA), mediates binding septins to but molecular determinants this interaction remained uncertain. We demonstrate short microtubule-associated protein (MAP)-like motif unique SEPT9 isoform...
SUMMARY The cytoskeletal protein actin is crucial for cell shape and integrity throughout eukaryotes. Actin filaments perform essential biological functions, including muscle contraction, division tissue morphogenesis. These diverse activities are achieved through the ability of to be arranged into architectures, but a detailed appreciation dynamic organizational state has been hindered by available tools. Fluorescence polarization microscopy uniquely placed measuring organization exploiting...
Abstract Septin GTP-binding proteins contribute essential biological functions that range from the establishment of cell polarity to animal tissue morphogenesis. Human septins in cells form hetero-octameric septin complexes containing ubiquitously expressed SEPT9. Despite established role SEPT9 mammalian development and human pathophysiology, biochemical biophysical studies have relied on monomeric thus not recapitulating its native assembly into complexes. We a protocol enabled first-time...
Abstract Septins are cytoskeletal proteins conserved from algae and protists to mammals. Septin knock-out animals have established that septins essential for animal physiology, but their molecular function remains elusive. A unique feature of is presence as heteromeric complexes polymerize into filaments in solution on lipid membranes. Although associate extensively with actin-based structures cells, whether actin-decorating organize if septin organization impacts not known. Customizing a...
Abstract Septins are conserved cytoskeletal proteins that regulate cell cortex mechanics. The mechanisms of their interactions with the plasma membrane remain poorly understood. Here we show by cell-free reconstitution binding requires electrostatic septins anionic lipids and promotes ordered self-assembly fly into filamentous meshworks. Transmission electron microscopy reveals both mammalian form arrays single paired filaments. Atomic force quartz crystal microbalance demonstrate filaments...
Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect role of environment (ionic conditions, protein lipid compositions, curvature) different partners associated with specific reshaping processes, we undertake in vitro bottom-up approaches. In recent years, there has been keen interest revealing septin proteins major diseases. Septins are essential ubiquitous cytoskeletal that interact membrane. They implicated cell division, motility,...
Septins are a family of conserved eukaryotic GTP-binding proteins that can form cytoskeletal filaments and higher-order structures from hetero-oligomeric complexes. They interact with other components the cell membrane to participate in important cellular functions such as migration division. Due complexity septins' many interactions, large number septin genes (13 humans), ability septins complexes different subunit compositions, cell-free reconstitution is vital strategy understand basics...
Septins are a family of conserved eukaryotic GTP-binding proteins that can form cytoskeletal filaments and higher-order structures from hetero-oligomeric complexes. They interact with other components the cell membrane to participate in important cellular functions such as migration division. Due complexity septins' many interactions, large number septin genes (13 humans), ability septins complexes different subunit compositions, cell-free reconstitution is vital strategy understand basics...
Abstract Septins, a family of GTP-binding proteins assembling into higher order structures, interface with the membrane, actin filaments and microtubules, which positions them as important regulators cytoarchitecture. Septin 9 (Sept9), is frequently overexpressed in tumors mutated hereditary neuralgic amyotrophy (HNA), mediates binding septins to but molecular determinants this interaction remained uncertain. We demonstrate that short MAP-like motif unique Sept9 isoform 1 (Sept9_i1) drives...
Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect role of environment (ionic conditions, protein lipid compositions, curvature) different partners associated with specific reshaping processes, we undertake in vitro bottom-up approaches. In recent years, there has been keen interest revealing septin proteins major diseases. Septins are essential ubiquitous cytoskeletal that interact membrane. They implicated cell division, motility,...