A5083590326- Proteins in Food Systems
- DNA and Nucleic Acid Chemistry
- Protein Interaction Studies and Fluorescence Analysis
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Enzyme Production and Characterization
- Iron Metabolism and Disorders
- Phytase and its Applications
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Protease and Inhibitor Mechanisms
- Biochemical effects in animals
- Surfactants and Colloidal Systems
- Hemoglobin structure and function
- Amino Acid Enzymes and Metabolism
- Diet, Metabolism, and Disease
- Radioactive element chemistry and processing
- Trace Elements in Health
- Chemical Synthesis and Analysis
- Glycogen Storage Diseases and Myoclonus
- Radioactive contamination and transfer
- Glycosylation and Glycoproteins Research
- Digestive system and related health
- Carbohydrate Chemistry and Synthesis
Denso (Japan)
2021-2022
Kyoto University
2003-2020
Curtin University
2020
Osaka Prefectural Government
2018
Denso (United States)
2011-2017
Sophia University
2014-2015
Takeda (Japan)
2012-2013
Noguchi Hospital
2013
Nitto (Japan)
2013
Aoyama Gakuin University
2012
A critical role for the small GTPase Rho and one of its targets, p160ROCK (a Rho-associated coiled coil-forming protein kinase), in neurite remodeling was examined neuroblastoma N1E-115 cells. Using wild-type a dominant-negative form p160ROCK-specific inhibitor, Y-27632, we show here that activation is necessary sufficient agonist-induced retraction cell rounding. The accompanied by elevated phosphorylation myosin light chain disassembly intermediate filaments microtubules. Y-27632 blocked...
Female mice lacking the gene encoding prostaglandin (PG) E 2 receptor subtype EP (EP −/− ) become pregnant and deliver their pups at term, but with a much reduced litter size. A decrease in ovulation number fertilization rate were observed females without difference of uterus to support implantation wild-type embryos. Treatment gonadotropins induced mRNA expression cumulus cells ovarian follicles mice. The immature cumuli oophori from expanded vitro response both follicle-stimulating hormone...
Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein involved in signaling from Rho to actin cytoskeleton.However, vivo functions of each ROCK isoform remain largely unknown.We generated mice deficient by gene targeting.ROCK-II ؊/؊ embryos were found at expected Mendelian frequency until 13.5 days postcoitum, but approximately 90% died thereafter utero.ROCK-II both genders that survived born runts, subsequently developed without gross abnormality,...
Journal Article Early electrical remodeling in rabbit pulmonary vein results from trafficking of intracellular SK2 channels to membrane sites Get access Nazira Özgen, Özgen aCenter for Molecular Therapeutics, College Physicians and Surgeons Columbia University, New York, United StatesbDepartment Pharmacology, States Search other works by this author on: Oxford Academic PubMed Google Scholar Wen Dun, Dun bDepartment Eugene A. Sosunov, Sosunov Evgeny P. Anyukhovsky, Anyukhovsky Masanori...
With the aim of discovering a selective kinase inhibitor targeting pan-RAF inhibition, we designed novel 1,3-benzothiazole derivatives based on our thiazolo[5,4-b]pyridine class RAF/VEGFR2 1 and developed regioselective cyclization methodology for C-7-substituted scaffold utilizing meta-substituted anilines. Eventually, selected 7-cyano derivative 8B (TAK-632) as development candidate confirmed its binding mode by cocrystal structure with BRAF. Accommodation group into BRAF-selectivity...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural evidence for a pH-sensitive dilysine trigger in the hen ovotransferrin N-lobe: Implications transferrin iron releaseJohn C. Dewan, Bunzo Mikami, Masaaki Hirose, and James SacchettiniCite this: Biochemistry 1993, 32, 45, 11963–11968Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe 2.0-.ANG. resolution structure of soybean .beta.-amylase complexed with .alpha.-cyclodextrinBunzo Mikami, Edward J. Hehre, Mamoru Sato, Yukiteru Katsube, Masaaki Hirose, Yuhei Morita, and James C. SacchettiniCite this: Biochemistry 1993, 32, 27, 6836–6845Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July...
To develop RAF/VEGFR2 inhibitors that bind to the inactive DFG-out conformation, we conducted structure-based drug design using X-ray cocrystal structures of BRAF, starting from an imidazo[1,2-b]pyridazine derivative. We designed various [5,6]-fused bicyclic scaffolds (ring A, 1–6) possessing anilide group forms two hydrogen bond interactions with Cys532. Stabilizing planarity this and nitrogen atom on six-membered ring scaffold was critical for enhancing BRAF inhibition. The selected...
Ovalbumin, a non-inhibitory member of serine proteinase inhibitors (serpin), is transformed into heat-stabilized form, S-ovalbumin, under elevated pH conditions. The structural mechanism for the S-ovalbumin formation has long been puzzling question in food science and serpin biology. On basis commonly observed thermostabilization by insertion reactive center loop proximal β-sheet, most widely accepted hypothetical model included partial insertion. Here we demonstrate, first time, crystal...
The utility of various kinds acid salts azole derivatives as promoters for the condensation a nucleoside phosphoramidite and is investigated. Among salts, N-(phenyl)imidazolium triflate, N-(p-acetylphenyl)imidazolium N-(methyl)benzimidazolium benzimidazolium perchlorate have shown extremely high reactivity in liquid phase. These reagents serve powerful activators deoxyribonucleoside 3'-(allyl N,N-diisopropylphosphoramidite)s or 3'-(2-cyanoethyl employed preparation deoxyribonucleotides,...
The conformation of the fully disulfide-reduced state human serum albumin was investigated by tryptophan fluorescence spectrum, CD analyses, and size-exclusion chromatography. Both reduction native disulfide-bonded form under nondenaturing conditions refolding urea-denatured reduced yielded almost exactly same with partially folded unique that clearly distinguished from either or denatured state. In addition, interconversion between reversible each other; reoxidation, converted to form....
The three-dimensional crystal structure of hen apo-ovotransferrin has been solved by molecular replacement and refined simulated annealing restrained least squares to a 3.0-Å resolution. final model, which comprises 5312 protein atoms (residues 1 686) 28 carbohydrate (from two monosaccharides attached Asn<sup>473</sup>), gives an <i>R</i>-factor 0.231 for the 11,989 observed reflections between 20.0- In structure, both empty iron binding clefts are in open conformation, lending weight theory...
Journal Article Exanthem Subitum and Antibody to Human Herpesvirus-6 Get access Kohji Ueda, Ueda Please address requests for reprints Dr. Department of Pediatrics, Faculty Medicine, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812, Japan. Search other works by this author on: Oxford Academic PubMed Google Scholar Koichi Kusuhara, Kusuhara Mizuo Hirose, Hirose Kenji Okada, Okada Chiaki Miyazaki, Miyazaki Ken Tokugawa, Tokugawa Minako Nakayama, Nakayama Yamanishi The Infectious...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTO-Allyl protection of guanine and thymine residues in oligodeoxyribonucleotidesYoshihiro Hayakawa, Masaaki Hirose, Ryoji NoyoriCite this: J. Org. Chem. 1993, 58, 20, 5551–5555Publication Date (Print):September 1, 1993Publication History Published online1 May 2002Published inissue 1 September 1993https://pubs.acs.org/doi/10.1021/jo00072a050https://doi.org/10.1021/jo00072a050research-articleACS PublicationsRequest reuse permissionsArticle...
Transferrins bind Fe3+ very tightly in a closed interdomain cleft by the coordination of four protein ligands (Asp60, Tyr92, Tyr191, and His250 ovotransferrin N-lobe) synergistic anion, physiologically bidentate CO32−. Upon Fe3+uptake, transferrins undergo large scale conformational transition: apo structure with an opening is transformed into holo structure, implying initial binding open form. To solve Fe3+-loaded, domain-opened N-lobe crystal that had been grown as form was soaked...