A5041459633- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Neuroscience and Neuropharmacology Research
- IL-33, ST2, and ILC Pathways
- Exercise and Physiological Responses
- MicroRNA in disease regulation
- Pancreatitis Pathology and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Pancreatic and Hepatic Oncology Research
- Galectins and Cancer Biology
- Hair Growth and Disorders
- Biochemical effects in animals
- Protein Degradation and Inhibitors
- Hemoglobin structure and function
- Polyamine Metabolism and Applications
- Atherosclerosis and Cardiovascular Diseases
Inha University
2013-2024
Inha University Hospital
2012-2023
La Jolla Institute for Immunology
2004-2010
Heinrich Heine University Düsseldorf
1997
Abstract Mesenchymal stem cells (MSCs) can modulate lymphocyte proliferation and function. One of the immunomodulatory functions MSCs involves CD4 + CD25 FoxP3 regulatory T (Tregs), which negatively regulate inflammatory responses. MSC-mediated Treg induction is supposed to be regulated by mechanisms requiring both soluble cell contact-dependent factors. Although involvement factors has been revealed, in remain unclear. We attempted identify molecule(s) other than secreted that are...
Generation of reactive oxygen species (ROS) by NADPH oxidase 4 (Nox4) induces the proliferation and migration adipose-derived stem cells (ASCs). However, functional role mitochondrial ROS (mtROS) generation in ASCs is unknown. Therefore, we have investigated whether hypoxia differentiation via generation. We also tried to identify cellular mechanisms underlying adipocyte differentiation. Hypoxia (2%) generators, such as antimycin rotenone, induced differentiation, which was attenuated an...
Abstract Signaling through the TCR and costimulatory signals primarily control transcription of IL-2 gene in naive T cells. The minimal promoter necessary for this expression lies proximal, between −300 start site. We had previously shown that activation arylhydrocarbon receptor (AHR), a member bHLH-PAS family factors, leads to increased mRNA murine fetal thymocytes. AHR is abundant thymus may play role development immune system. Moreover, its overactivation by chemicals such as dioxins...
Bone marrow-derived mesenchymal stem cells (MSCs) have immunomodulatory properties and can suppress exaggerated pro-inflammatory immune responses.Although the exact mechanisms remain unclear, a variety of soluble factors are known to contribute MSC-mediated immunosuppression.However, functional redundancy in immunosuppressive MSCs indicates that other uncharacterized could be involved.Galectin-9, member β-galactoside binding galectin family, has emerged as an important regulator innate...
Mouse bone marrow-derived clonal mesenchymal stem cells (mcMSCs), which were originated from a single cell by subfractionation culturing method, are recognized as new paradigm for therapy featured with its homogenous population. Next to proven therapeutic effects against pancreatitis, in the current study we demonstrated that mcMSCs showed significant dextran sulfate sodium (DSS)-induced experimental colitis model supported anti-inflammatory and restorative activities. significantly reduced...
Minha Kim, So-Dam Kyoung In Eun Hae Jeon, Min Gee Yu-Ree Lim, Enkhmaa Lkhagva-Yondon, Yena Oh, Kwangmin Na, Young Cheul Chung, Byung Kwan Jin, Yun Seon Song and Myung-Shin Jeon. Exp Neurobiol 2021;30:155-69. https://doi.org/10.5607/en20062
Although there was no impairment in IL-2 secretion and proliferation of Fyn-deficient naïve CD4 cells after stimulation with antigen antigen-presenting cells, these anti-CD3 anti-CD28 revealed profound defects. Crosslinking purified wild-type activated Lck initiated the signaling cascade downstream Lck, including phosphorylation ZAP-70, LAT, PLC-γ1; calcium flux; dephosphorylation nuclear translocation factor T (NFAT)p. All events were diminished severely by CD3 crosslinking. Coaggregation...
Since the discovery of immunomodulation property mesenchymal stem cells (MSCs) about a decade ago, it has been extensively investigated whether MSCs can be used for treatment immune-related diseases, such as graft-versus-host disease (GvHD). However, how to evaluate efficacy human clinical trial is still unclear. We an MHC-mismatched model GvHD (B6 into BALB/c). Surprisingly, administration (hMSCs) could reduce GvHD-related mortality mouse recipients and xenogeneically inhibit T-cell...
Mesenchymal stromal cells (MSCs) are known to suppress T-cell activation and proliferation. Several studies have reported that MSCs CD25 expression in T cells. However, the molecular mechanism underlying MSC-mediated suppression of has not been fully examined. Here, we investigated mTOR pathway, which is involved We showed inhibited expression, was restored presence an inducible nitric oxide synthase (iNOS) inhibitor. Since mRNA inhibited, focused on determining whether modulated components...
AIM:To characterize single-cell-derived mouse clonal mesenchymal stem cells (mcMSCs) established with bone marrow samples from three different strains. METHODS:We mcMSC lines using subfractionation culturing method obtained long bones.These were characterized by measuring cell growth, surface epitopes, differentiation potential, lineage-specific gene expression and T-cell suppression capability.Nonclonal MSCs isolated the conventional gradient centrifugation used as controls. RESULTS:All...
CT-P13 is the first biosimilar monoclonal antibody to infliximab, and was recently approved in European Union, Japan, Korea, USA for all six indications of infliximab. However, studies directly assessing biologic activity versus inflximab context inflammatory bowel disease [IBD] are still scanty. In present study, we aimed compare biological activities infliximab with specific focus on intestinal cells so as gain insight into potential biosimilarity these two agents treatment IBD.CT-P13 were...
AbstractThe induction process of the galactose regulon has been intensively studied, but until now nature inducer remained unknown. We have analyzed a ∆gal7 mutant yeast Kluyveromyces lactis, which lacks galactotransferase activity and is able to express genes Gal/Lac also in absence galactose. found that this expression semiconstitutive undergoes strong during stationary phase. The gal1-209 mutant, reduced kinase retains its positive regulatory function, shows constitutive β-galactosidase,...
Atopic dermatitis (AD) is a chronic inflammatory skin disease. A hallmark of AD dry itchy that results from defects in the epidermal barrier function. Aloe vera used widely to promote general health and administered topically treat conditions such as eczema, burns wounds. However, effects on were not fully elucidated. In this study, we investigated oral administration processed gel (PAG) containing low molecular weight polysaccharides ovalbumin (OVA)-induced mice. Oral PAG suppressed total...
Abstract Mesenchymal stem cells (MSCs) have great therapeutic advantages due to their immunosuppressive properties. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor whose signaling plays an important role in the immune system. AHR may be involved regulation of MSC-associated immunomodulatory functions. However, mechanisms by which controls functions MSCs are not well understood. Here, we report that Ahr -deficient show decreased efficacy against...
(PC) has been reported to have antimicrobial and anti-inflammatory properties, but its potential as a neuroprotective agent in mouse model of cerebral ischemia not explored. Considering neuroglobin (Ngb), an endogenous factor, novel approach neuroprotection, this study, Ngb promoter activity, expression changes, antioxidant protection by PC extract (PCE) component compounds (PCCs) were analyzed oxygen-glucose deprivation (OGD)-treated neurons. In vivo analysis involved transient middle...
Abstract Atopic dermatitis is a chronic and relapsing inflammatory skin disease that treated with immunosuppressants. However, long‐term use of immunosuppressants may cause toxicity severe side‐effects. To confirm the efficacy safety clonal mesenchymal stem cell therapy, we performed investigator‐initiated clinical trials observation in five adult patients moderate to atopic was refractory conventional treatments. The response assessment values such as Eczema Area Severity Index (EASI)...
Mesenchymal stem cells (MSCs) are used to treat autoimmune or inflammatory diseases. Our aim was determine the immunomodulatory mechanisms elicited by MSCs during inflammation.We cocultured with peripheral blood mononuclear for a mixed lymphocyte reaction stimulated them phytohemagglutinin. Morphological changes of and secretion acetylcholine (ACh) from were measured. The effects an ACh antagonist agonist on proliferation proinflammatory-cytokine production determined. milieu created...
Bone marrow-derived mesenchymal stem cells (MSCs) are multipotent, with the ability to differentiate into different cell types.Additionally, immunomodulatory activity of MSCs can downregulate inflammatory responses.The use repair injured tissues and treat inflammation, including in neuroimmune diseases, has been extensively explored.Although have emerged as a promising resource for treatment attempts define molecular properties limited by heterogeneity MSC populations.We recently developed...
Anti-interleukin-33 (anti-IL-33) and anti-Siglec-F antibodies have potent anti-allergic effects on murine allergic asthma rhinitis induce eosinophil apoptosis.We aimed to determine whether post-sensitization with anti-IL-33/anti-Siglec-F treatments exhibited more compared individual in a model.Twenty-five BALB/c mice were separated into five groups (n = 5): Group A (control), B (ovalbumin [OVA] challenge), C (OVA + anti-IL-33), D anti-Siglec-F), E anti-IL-33 anti-Siglec-F). Serum total/...