A5043827609- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Herbal Medicine Research Studies
- Hemiptera Insect Studies
- Neuroscience and Neural Engineering
- Neuroscience of respiration and sleep
- Alzheimer's disease research and treatments
- Estrogen and related hormone effects
- Adipose Tissue and Metabolism
- Nicotinic Acetylcholine Receptors Study
- Plant and Biological Electrophysiology Studies
- Amyotrophic Lateral Sclerosis Research
- Autophagy in Disease and Therapy
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Physiological and biochemical adaptations
- Menopause: Health Impacts and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Nuclear Receptors and Signaling
- Mitochondrial Function and Pathology
- Connexins and lens biology
- Extracellular vesicles in disease
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- MicroRNA in disease regulation
UCLouvain Saint-Louis Brussels
2019-2024
China Pharmaceutical University
2022-2024
Saint Louis University
2016-2023
Saint Louis University
2019
University of Calgary
2009-2017
Alberta Children's Hospital
2016
University of Alberta
2005-2007
Center for Neurosciences
2007
Stony Brook University
2004
Shanghai University of Traditional Chinese Medicine
1984
Silver nanoparticles (AgNPs), owing to their effective antimicrobial properties, are being widely used in a broad range of applications. These include, but not limited to, antibacterial materials, the textile industry, cosmetics, coatings various household appliances and medical devices. Despite extensive use, little is known about AgNP safety toxicity vis-à-vis human animal health. Recent studies have drawn attention towards potential neurotoxic effects AgNPs, however, primary cellular...
Mercury is a well-known neurotoxin implicated in wide range of neurological or psychiatric disorders including autism spectrum disorders, Alzheimer’s disease, Parkinson’s epilepsy, depression, mood and tremor. Mercury-induced neuronal degeneration thought to invoke glutamate-mediated excitotoxicity, however, the underlying mechanisms remain poorly understood. Here, we examine effects various mercury concentrations (including pathological levels present human plasma cerebrospinal fluid) on...
The objective of this work was to test the synergistic effects substrate stiffness, electro-conductivity, composition and electrical stimulation on morphology, alignment directional neurite outgrowth neuron-like PC12 cells. use exogenous has emerged as a promising new intervention promote neural regeneration following injury. For critical gap size nerve injuries, permissive biomaterial coupled may be needed provide guidance support for outgrowth. Thus, combinatorial properties need...
Abstract The neural functions of adropin, a secreted peptide highly expressed in the brain, have not been investigated. In humans, adropin is astrocytes and peaks during critical postnatal periods brain development. Gene enrichment analysis transcripts correlating with expression suggests processes relevant to aging-related neurodegenerative diseases that vary age dementia state, possibly indicating survivor bias. people aged <40 y ‘old-old’ (>75 y) diagnosed dementia, correlates...
During hypoxia, the level of adenosine in carotid bodies increases as a result ATP catabolism and efflux via transporters. Using Ca2+ imaging, we found that adenosine, acting A2A receptors, triggered rise cytoplasmic [Ca2+] ([Ca2+]i) type I (glomus) cells rat bodies. The response could be mimicked by forskolin (but not its inactive analog), abolished PKA inhibitor H89. Simultaneous measurements membrane potential (perforated patch recording) [Ca2+]i showed adenosine-mediated was accompanied...
Concerns have risen regarding the potential side effects of clinical exposure pediatric population to inhalational anesthetics, and how they might impact cognitive, learning, memory functions. However, neither mechanisms anesthetic cytotoxicity, nor protective strategies, yet been fully explored. In this study, we examined whether two most commonly used sevoflurane desflurane, affect neuronal viability synaptic network assembly between cultured rat cortical neurons.Primary neuron cultures...
Taurine is a sulfur-containing amino acid that widely expressed throughout the human brain, heart, retina, and muscle tissues. deficiency associated with cardiomyopathy, renal dysfunction, abnormalities of developing nervous system, epilepsy which suggests role specific to excitable Like vertebrates, invertebrates maintain high levels taurine during embryonic larval development, decline aging, indicating potential developmental role. Notwithstanding its extensive presence throughout,...
Abstract Estrogen plays fundamental roles in nervous system development and function. Traditional studies examining the effect of estrogen brain have focused on nuclear receptors (ERs), ERα ERβ. Studies related to extranuclear, membrane-bound G-protein-coupled ER (GPER/GPR30) revealed a neuroprotective role for GPER mature neurons. In this study, we investigated differential effects activation primary rat embryonic day 18 (E18) hippocampal cortical Microscopy imaging, multielectrode array...
Summary During hypoxia, ATP was released from type I (glomus) cells in the carotid bodies. We studied action of on intracellular Ca 2+ concentration ([Ca ] i ) dissociated rat bodies using a imaging technique. did not affect resting [Ca but strongly suppressed hypoxia‐induced elevations cells. The order purinoreceptor agonist potency inhibiting hypoxia response 2‐methylthioATP > ADP ≫ α, β‐methylene UTP, implicating involvement P2Y 1 receptors. Simultaneous measurements membrane potential...
Accumulated soluble amyloid beta- (A β -) induced aberrant neuronal network activity may directly contribute to cognitive deficits, which are the most outstanding characteristics of Alzheimer’s disease (AD). The entorhinal cortex (EC) is one earliest affected brain regions in AD. Impairments EC neurons responsible for deficits However, little effort has been made investigate effects A on discharge properties vivo . present study was designed examine 1−42 neurons, using extracellular single...
Cysteine string protein (CSPalpha) is a synaptic vesicle that displays unique anti-neurodegenerative properties. CSPalpha member of the conserved J family, also called Hsp40 (heat shock 40 kDa) whose importance in folding has been recognized for many years. Deletion mice results knockout are normal first 2-3 weeks life followed by an unexplained presynaptic neurodegeneration and premature death. How prevents currently not known. As neuroprotective protein, represents promising therapeutic...
Connections between neurons called synapses are the key components underlying all nervous system functions of animals and humans. However, important genetic information on formation plasticity one type, electrical (gap junction-mediated) synapse, is understudied in many invertebrates. In present study, we set forth to identify characterize gap junction-encoding gene innexin central (CNS) mollusc pond snail Lymnaea stagnalis (L. stagnalis). With PCR, 3' 5' RACE, BLAST searches, identified...
Nervous system functions in all animals rely upon synaptic connectivity that is established during early development. Whereas cell–cell signaling plays a critical role establishing synapse specificity, the involvement of extrinsic growth factors cannot, however, be undermined. We have previously demonstrated trophic are required for excitatory but not inhibitory formation between Lymnaea neurons. Moreover, absence factors, neurons from number species establish inappropriate synapses, which...
Abstract Current treatment regimes for a variety of mental disorders involve various selective serotonin reuptake inhibitors such as Fluoxetine (Prozac). Although these drugs may ‘manage’ the patient better, there has not been significant change in paradigm over years and neither have outcomes improved. There is also considerable debate to effectiveness their potential side‐effects on neuronal architecture function. In this study, using mammalian cortical neurons, dorsal root ganglia cell...
Astrocytes activate and crosstalk with neurons influencing inflammatory responses following ischemic stroke. The distribution, abundance, activity of microRNAs in astrocytes-derived exosomes after stroke remains largely unknown. In this study, were extracted from primary cultured mouse astrocytes via ultracentrifugation, exposed to oxygen glucose deprivation/re‑oxygenation injury mimic experimental SmallRNAs astrocyte-derived sequenced, differentially expressed randomly selected verified by...
Abstract Pituitary adenylate cyclase‐activating polypeptide (PACAP)‐deficient mice are prone to sudden neonatal death and have reduced respiratory response hypoxia. Here we found that PACAP‐38 elevated cytosolic [Ca 2+ ] ([Ca i ) in the oxygen sensing type I cells but not glial‐like II (sustentacular) of rat carotid body. This action PACAP could be mimicked by vasoactive intestinal peptide was abolished 6‐38, implicating involvement PAC 1 receptors. H89, a protein kinase A (PKA) inhibitor...
Abstract Synapse formation and plasticity depend on nuclear transcription site-specific protein targeting, but the molecular mechanisms that coordinate these steps have not been well defined. The MEN1 tumor suppressor gene, which encodes menin, is known to induce synapse in CNS. This synaptogenic function has conserved across evolution, however underlying remain unidentified. Here, using central neurons from invertebrate Lymnaea stagnalis , we demonstrate menin coordinates subunit-specific...