A5072367040- Osteoarthritis Treatment and Mechanisms
- Shoulder Injury and Treatment
- Tendon Structure and Treatment
- Shoulder and Clavicle Injuries
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Nerve injury and regeneration
- Animal Genetics and Reproduction
- Orthopedic Surgery and Rehabilitation
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Immune Response and Inflammation
- MicroRNA in disease regulation
- Fibroblast Growth Factor Research
- Neurogenesis and neuroplasticity mechanisms
- Sports injuries and prevention
- Zebrafish Biomedical Research Applications
- Inflammatory mediators and NSAID effects
- interferon and immune responses
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- Connective tissue disorders research
- Developmental Biology and Gene Regulation
- Proteoglycans and glycosaminoglycans research
- Congenital heart defects research
University of Utah
2012-2025
Cancer Research Foundation
2005
Pennsylvania State University
2005
Emory University
1999-2003
Lighter variations of pigmentation in humans are associated with diminished number, size, and density melanosomes, the pigmented organelles melanocytes. Here we show that zebrafish golden mutants share these melanosomal changes encodes a putative cation exchanger slc24a5 (nckx5) localizes to an intracellular membrane, likely melanosome or its precursor. The human ortholog is highly similar sequence functional zebrafish. evolutionarily conserved ancestral allele coding polymorphism...
Chondroitin sulfate proteoglycans (CS-PGs) expressed by reactive astrocytes may contribute to the axon growth-inhibitory environment of injured CNS. The specific potentially inhibitory CS-PGs present in areas gliosis, however, have yet be thoroughly examined. In this study, we used immunohistochemistry, combined immunohistochemistry– situ hybridization, immunoblot analysis, and reverse transcription-PCR examine expression an vivo model gliosis: that is, glial scar, after cortical injury....
The zebrafish is a powerful experimental system for uncovering gene function in vertebrate organisms. Nevertheless, studies the have been limited by approaches available eliminating function. Here we present simple and efficient methods inducing, detecting, recovering mutations at virtually any locus zebrafish. Briefly, double-strand DNA breaks are induced of interest synthetic nucleases, called TALENs. Subsequent host repair lesions leads to generation insertion deletion targeted locus. To...
Mutations affecting the seemingly unrelated gene products, SepN1, a selenoprotein of unknown function, and RyR1, major component ryanodine receptor intracellular calcium release channel, result in an overlapping spectrum congenital myopathies. To identify immediate developmental molecular roles SepN RyR vivo, loss-of-function effects were analyzed zebrafish embryo. These studies demonstrate two proteins are required for same cellular differentiation events needed normal fluxes is physically...
Failure of healing after rotator cuff repair (RCR) is common. The purpose the current study was to evaluate effect systemic estrogen or testosterone supplementation on tendon RCR. Seventy-two adult male mice were utilized for all experiments. supraspinatus transected and repaired with 6-0 Prolene suture left shoulder 51 animals. Mice segregated into three groups postoperative: (1) vehicle group (VG; n = 18), (2) (EST; 17), (3) (TST; 16). An unrepaired control (unrepaired, 21) did not have...
Objectives Chondrocyte metabolic dysfunction plays an important role in osteoarthritis (OA) development during aging and obesity. Protein post‐translational modifications (PTMs) have recently emerged as regulator of cellular metabolism. We aim to study one type PTM, lysine malonylation (MaK) its Sirt5 OA development. Methods Human mouse cartilage tissues were used measure SIRT5 MaK levels. Both systemic cartilage‐specific conditional knockout models subject high‐fat diet (HFD) treatment...
How inflammatory signalling contributes to osteoarthritis (OA) susceptibility is undetermined. An allele encoding a hyperactive form of the Receptor Interacting Protein Kinase 2 (RIPK2) proinflammatory intermediate has been associated with familial OA. To test whether altered nucleotide-binding oligomerisation domain (NOD)/RIPK2 pathway activity causes heightened OA susceptibility, we investigated variants affecting additional components are determine Ripk2104Asp disease sufficient account...
Abstract Members of the Slit family regulate axon guidance and cell migration. To date, three vertebrate slit1 genes have been identified in mammals orthologs two, slit2 slit3 , zebrafish. Here, we describe cloning full‐length cDNAs for two zebrafish slit orthologs, slit1a slit1b . Both predicted proteins contain conserved motifs that characterize other Slits. are both expressed midline, hypochord, telencephalon, hindbrain. Apart from these shared expression domains, however, their patterns...
Osteoarthritis (OA) is a common debilitating disease characterized by abnormal remodeling of the cartilage and bone articular joint. Ameliorating therapeutics are lacking due to limited understanding molecular pathways affecting initiation progression. Notably, although link between inflammation overt OA well established, role as driver occurrence highly disputed. We analyzed family with dominant inheritance early-onset found that affected individuals harbored rare variant allele encoding...
SUMMARY The vertebrate heart is one of the first organs to form, and its early function morphogenesis are crucial for continued embryonic development. Here we analyze effects loss Heart adaptor protein 1 (Hadp1), which show required normal zebrafish heart. Hadp1 a pleckstrin homology (PH)-domain-containing whose expression enriched in cardiomyocytes. Knockdown hadp1 embryos reduced cardiac contractility altered late myocyte differentiation. By using optical mapping submaximal levels...
Osteoporosis is a common skeletal disorder characterized by deterioration of bone tissue. The set genetic factors contributing to osteoporosis not completely specified. High-risk pedigrees were analyzed identify genes that may confer susceptibility disease. Candidate predisposition variants identified initially whole exome sequencing affected-relative pairs, approximately cousins, from 10 pedigrees. Variants filtered on the basis population frequency, concordance between pairs affecting gene...
Chondrocyte metabolic dysfunction plays an important role in osteoarthritis (OA) development during aging and obesity. Protein post-translational modifications (PTMs) have recently emerged as regulator of cellular metabolism. We aim to study one type PTM, lysine malonylation (MaK) its Sirt5 OA development.
SH2-domain-containing inositol phosphatase 2 (SHIP2) belongs to a small family of phosphoinositide 5-phosphatases that help terminate intracellular signaling initiated by activated receptor tyrosine kinases. Mammalian SHIP2 is viewed primarily as an attenuator insulin and has become prominent candidate target for therapeutic agents are designed augment signaling. Despite this view, no pathway yet been demonstrated being affected directly function in vivo, vitro studies indicate the protein...
Erosive hand osteoarthritis (OA) is a severe and rapidly progressing subset of OA. Its etiology remains largely unknown, which has hindered development successful treatments. This study was undertaken to test the hypothesis that erosive OA demonstrates familial clustering in large statewide population linked genealogical records, determine association potential risk factors with OA.Patients diagnosed as having were identified by searching 4,741,840 unique medical records from comprehensive...
ABSTRACT Multipotent progenitor populations are necessary for generating diverse tissue types during embryogenesis. We show the RNA polymerase-associated factor 1 complex (Paf1C) is required to maintain multipotent progenitors of neural crest (NC) lineage in zebrafish. Mutations affecting each Paf1C component result near-identical NC phenotypes; alyron mutant embryos carrying a null mutation paf1 were analyzed detail. In absence zygotic function, definitive premigratory arise but fail...