A5027231792- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Inflammatory Bowel Disease
- Gastrointestinal motility and disorders
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Microscopic Colitis
- Diet and metabolism studies
- Colorectal Cancer Surgical Treatments
- Veterinary medicine and infectious diseases
- Esophageal Cancer Research and Treatment
- Mycobacterium research and diagnosis
- Food composition and properties
- Diverticular Disease and Complications
- Plant Pathogenic Bacteria Studies
- Pleural and Pulmonary Diseases
- Surgical site infection prevention
- Eosinophilic Esophagitis
- Oral Health Pathology and Treatment
- Anaerobic Digestion and Biogas Production
- Birth, Development, and Health
- Viral gastroenteritis research and epidemiology
- Lung Cancer Diagnosis and Treatment
- Tryptophan and brain disorders
Hunter Medical Research Institute
2021-2024
University of Newcastle Australia
2021-2024
National Health and Medical Research Council
2021-2024
The University of Queensland
2015-2023
Translational Research Institute
2016-2023
APC Microbiome Institute
2019-2023
University College Cork
2019-2023
Australasian Gastro Intestinal Research Foundation
2022-2023
Newcastle University
2022
Brisbane School of Theology
2020
Proteus spp, Gram-negative facultative anaerobic bacilli, have recently been associated with Crohn's disease (CD) recurrence after intestinal resection. We investigated the genomic and functional role of as a gut pathogen in CD.Proteus spp abundance was assessed by ure gene-specific polymerase chain 54 pairs fecal samples 101 biopsies from patients CD healthy controls. The adherence, invasion, intracellular presence 2 distinct isolates mirabilis epithelial cells were evaluated using...
Emerging evidence suggests that microbiome-host crosstalk regulates intestinal immune activity and predisposition to inflammatory bowel disease (IBD). NF-κB is a master regulator of function validated target for the treatment IBD. Here, we identify five Clostridium strains suppress immune-mediated activation in epithelial cell lines, PBMCs, gut organoids from healthy human subjects patients with Cell-free culture supernatant bolteae AHG0001 strain, but not reference C. BAA-613 suppresses...
Despite advances in clinical proteomics, translating protein biomarker discoveries into use remains challenging due to the technical complexity of validation process. Targeted MS-based proteomics approaches such as parallel reaction monitoring (PRM) offer sensitive and specific assays for translation. In this study, we developed a multiplex PRM assay using Stellar mass spectrometry platform quantify 57 plasma proteins, including 21 FDA-approved proteins. Loading curves (11-points) were...
<italic>In vitro</italic> fermentation of wheat starch depends on amylose content in cooked but not granule forms, and shows that high is a promising source fermentable carbohydrate the large intestine.
With an increasing interest in the manipulation of methane produced from livestock cultivation, microbiome Australian marsupials provides a unique ecological and evolutionary comparison with 'low-methane' emitters. Previously, marsupial species were shown to be enriched for novel lineages Methanocorpusculum, as well Methanobrevibacter, Methanosphaera, Methanomassiliicoccales. Despite sporadic reports Methanocorpusculum stool samples various animal species, there remains little information on...
Abstract The mucosa-associated microbiota is widely recognized as a potential trigger for Crohn’s disease pathophysiology but remains largely uncharacterised beyond its taxonomic composition. Unlike stool microbiota, the functional characterisation of these communities using current DNA/RNA sequencing approaches constrained by relatively small microbial density on tissue, and overwhelming amount human DNA recovered during sample preparation. Here, we have used novel ex vivo approach that...
The production of SCFA and promotion bacteria, during fermentation soluble polysaccharides, depend on the initial composition host large intestinal microbiota more than type polysaccharide.
Typically, animal models studying gastrointestinal microbiotas compromised in early life have employed either germ-free animals or mice treated with a cocktail of antibiotics. Such studies intend to mimic scenarios infants born by caesarean section and/or subjected antibiotic treatment. However, the antibiotics used these are rarely prescribed infants. Therefore, an model was developed which murine microbiota severely disrupted clindamycin In this mouse model, we investigated extent...
Functional gastrointestinal disorders (FGIDs) encompass a range of complex conditions with similar clinical characteristics and no overt pathology. Recent recognition sub-clinical pathologies in FGIDs, conjunction physiological biochemical abnormalities including increased intestinal permeability, microbial profile alterations, differences metabolites extra-intestinal manifestations disease, call into question the designation these as 'functional'. This is despite significant heterogeneity...
Enterococcus faecalis is an early coloniser of the human infant gut and contributes to development intestinal immunity. To better understand functional capacity E. we constructed a broad host range RP4 mobilisable vector, pEHR513112, that confers chloramphenicol resistance used metaparental mating approach isolate AHG0090 from faecal sample collected healthy infant. We demonstrated genetically tractable could be manipulated using traditional molecular microbiology approaches. was comparable...
Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due their extensive and variable repertoire Restriction-Modification (R-M) systems. Non-replicating plasmids currently employed achieve insertional mutagenesis in . One limitations using such insertion vectors is presence within sequence various restriction sites, making them sensitive activity endogenous endonucleases encoded by target strain. For this reason, have been developed with aim methylating...
Hospital placement is essential training for medical interns, involving shift work and high-pressure environments. This can increase physiological psychological stress, which may be mediated by metabolites of microbial digestion (1) . Nutrients interest include those accessible to associated with altered signalling within the microbiota-gut-brain axis (MGBA) Fibre fermented gut microbes produce short-chain fatty acids (2) improved outcomes (3) Tryptophan, a precursor gut-derived serotonin ,...