A5107387450- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Nanopore and Nanochannel Transport Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Lung Cancer Research Studies
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Enzyme Structure and Function
- Biochemical and Structural Characterization
- RNA Interference and Gene Delivery
- stochastic dynamics and bifurcation
- Metabolism and Genetic Disorders
- Quantum-Dot Cellular Automata
- Bacterial Identification and Susceptibility Testing
- Mitochondrial Function and Pathology
- Force Microscopy Techniques and Applications
- Antibiotic Resistance in Bacteria
Centre National de la Recherche Scientifique
2012-2025
Institut de Biologie Structurale
2023-2025
CEA Grenoble
2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2025
Université Grenoble Alpes
2025
Université Claude Bernard Lyon 1
2011-2024
Lyon College
2024
Centre de Biologie Structurale
2019-2022
Laboratoire de Chimie Bactérienne
2012-2014
Aix-Marseille Université
2012-2014
Despite years of intensive research, much remains to be discovered understand the regulatory networks coordinating bacterial cell growth and division. The mechanisms by which Streptococcus pneumoniae achieves its characteristic ellipsoid-cell shape remain largely unknown. In this study, we analyzed interplay division paralogs DivIVA GpsB with ser/thr kinase StkP. We observed that deletion divIVA hindered elongation resulted in shortening rounding. By contrast, absence hampered triggered...
Eukaryotic-like serine/threonine-kinases are involved in the regulation of a variety physiological processes bacteria. In Streptococcus pneumoniae, deletion single serine/threonine-kinase gene stkP results an aberrant cell morphology suggesting that StkP participates pneumococcus division. To understand function StkP, we have engineered various strains expressing truncated or kinase-dead forms StkP. We show kinase activity, but also its extracellular and cytoplasmic domains per se, required...
Teichoic acids (TA) are linear phospho-saccharidic polymers and important constituents of the cell envelope Gram-positive bacteria, either bound to peptidoglycan as wall teichoic (WTA) or membrane lipoteichoic (LTA). The chemical composition TA varies greatly but presence both WTA LTA is highly conserved, hinting at an underlying fundamental function that distinct from their numerous specific roles in diverse organisms. We report here observation a periplasmic space Streptococcus pneumoniae...
Teichoic acids (TA) are linear phospho-saccharidic polymers and important constituents of the cell envelope Gram-positive bacteria, either bound to peptidoglycan as wall teichoic (WTA) or membrane lipoteichoic (LTA). The chemical composition TA varies greatly but presence both WTA LTA is highly conserved, hinting at an underlying fundamental function that distinct from their numerous specific roles in diverse organisms. We report here observation a periplasmic space Streptococcus pneumoniae...
Teichoic acids (TA) are linear phospho-saccharidic polymers and important constituents of the cell envelope Gram-positive bacteria, either bound to peptidoglycan as wall teichoic (WTA) or membrane lipoteichoic (LTA). The chemical composition TA varies greatly but presence both WTA LTA is highly conserved, hinting at an underlying fundamental function that distinct from their numerous specific roles in diverse organisms. We report here observation a periplasmic space Streptococcus pneumoniae...
ABSTRACT Eukaryotic-like membrane Ser/Thr protein kinases play a pivotal role in different aspects of bacterial physiology. In contrast to the diversity their extracellular domains, cytoplasmic catalytic domains are highly conserved. However, function long juxtamembrane domain (JMD), which connects transmembrane helix, remains elusive. this study, we investigated JMD kinase StkP cell division Streptococcus pneumoniae . We observed that deletion affected ability phosphorylate some its...
Accurate division site placement is essential for bacterial cells to produce viable daughter with proper size and appropriate functional features. In the opportunistic pathogen bacterium Streptococcus pneumoniae, positioning of has been shown depend on both protein MapZ chromosome segregation. However, nature this interplay molecular determinants guiding localization remained unclear. Here we demonstrated that positioned at cell equator, widest part body, rather than mid-cell. addition,...
The YvcK protein has been shown to be necessary for growth under gluconeogenic conditions in Bacillus subtilis. Amazingly, its overproduction rescues and morphology defects of the actin-like MreB deletion mutant by restoration PBP1 localization. In this work, we observed that was phosphorylated at Thr-304 kinase PrkC dephosphorylated cognate phosphatase PrpC. We show neither substitution threonine with a constitutively mimicking glutamic acid residue or phosphorylation-dead alanine nor prkC...
The mitochondrial voltage-dependent anion channel 1 (VDAC1) plays a central role in metabolism and apoptosis, which makes it promising therapeutic target. Nevertheless, molecular mechanisms governing VDAC1 functioning remain unclear. Small-molecule ligands specifically interacting with the provide an attractive way of exploring its structure-function relationships can possibly be used as founding stones for future drug-candidates. While around 30 have been identified over years, various...
Bacterial cell-wall hydrolases must be tightly regulated during bacterial cell division to prevent aberrant lysis and allow final separation of viable daughter cells. In a multidisciplinary work, we disclose the molecular dialogue between hydrolase LytB, wall teichoic acids, eukaryotic-like protein kinase StkP in Streptococcus pneumoniae. After characterizing peptidoglycan recognition mode by catalytic domain further demonstrate that LytB possesses modular organization allowing specific...
Two-component regulatory systems (TCS) are among the most widespread mechanisms that bacteria use to sense and respond environmental changes. In human pathogen Streptococcus pneumoniae , a total of 13 TCS have been identified many them linked pathogenicity. Notably, TCS01 strongly contributes pneumococcal virulence in several infection models. However, it remains one least studied pneumococci its functional role is still unclear. this study, we demonstrate cooperates with BceAB-type ABC...
Control of peptidoglycan assembly is critical to maintain bacterial cell size and morphology. Penicillin-binding proteins (PBPs) are crucial enzymes for the polymerization glycan strand and/or their cross-linking via peptide branches. Over last few years, it has become clear that PBP activity localization can be regulated by specific cognate regulators. The first regulator in Gram-positive bacteria was discovered human pathogen Streptococcus pneumoniae This regulator, named CozE, controls...
Protein phosphorylation is a key post-translational modification required for many cellular functions of the bacterial cell. Recently, we identified new protein-kinase, named UbK, in Bacillus subtilis that belongs to family protein-kinases widespread bacteria. In this study, analyze function UbK Streptococcus pneumoniae. We show displays tyrosine-kinase activity and autophosphorylates on unique tyrosine vivo. To get insights into its role, constructed set pneumococcal ubk mutants. Using...
Despite an extensive theoretical and numerical background, the translocation ratchet mechanism, which is fundamental for transmembrane transport of biomolecules, has never been experimentally reproduced at nanoscale. Only Sec61 bacterial type IV pilus pores were shown to exhibit a mechanism. Here we designed synthetic quantified its efficiency as nanopump. We measured frequency DNA molecules through nanoporous membranes showed that polycations trans side accelerated in ratchet-like fashion....
In Bacillus subtilis, the ribosome-associated GTPase CpgA is crucial for growth and proper morphology was shown to be phosphorylated in vitro by Ser/Thr protein kinase PrkC. To further understand function of Escherichia coli RsgA ortholog, CpgA, we first demonstrated that its activity stimulated association with 30 S ribosomal subunit. Then role phosphorylation analyzed. A single residue, threonine 166, identified mass spectrometry. Phosphoablative replacement this residue induces a decrease...
A particular class of tyrosine-kinases sharing no structural similarity with eukaryotic has been evidenced in a large array bacterial species. These are able to autophosphorylate on C-terminal tyrosine-rich motif. Their autophosphorylation shown play crucial role the biosynthesis or export capsular polysaccharide. The analysis first crystal structure staphylococcal tyrosine kinase CapB2 associated activating domain transmembrane modulator CapA1 had brought conclusive explanation for both and...
Abstract Bacterial shape and division rely on the dynamics of cell wall assembly, which involves regulated synthesis cleavage peptidoglycan. In ovococci, these processes are coordinated in an annular mid-cell region with nanometric dimensions. More precisely, cross-wall that is synthesized by divisome split to generate lateral wall, whose expansion insured insertion so-called peripheral peptidoglycan elongasome. Septum thus crucial remodeling events for ovococcal elongation. The structural...
Bacterial shape and division rely on the dynamics of cell wall assembly, which involves regulated synthesis cleavage peptidoglycan. In ovococci, these processes are coordinated within an annular mid-cell region with nanometric dimensions. More precisely, cross-wall synthesized by divisome is split to generate a lateral wall, whose expansion insured insertion so-called peripheral peptidoglycan elongasome. Septum are, thus, crucial remodeling events for ovococcal elongation. The structural...
Abstract Teichoic acids (TA) are linear phospho-saccharidic polymers and important constituents of the cell envelope Gram-positive bacteria, either bound to peptidoglycan as wall teichoic (WTA) or membrane lipoteichoic (LTA). The chemical composition TA varies greatly but presence both WTA LTA is highly conserved, hinting at an underlying fundamental function that distinct from their numerous specific roles in diverse organisms. We report here observation a periplasmic space Streptococcus...
Remodeling of the pneumococcal cell wall, carried out by peptidoglycan (PG) hydrolases, is imperative for maintaining bacterial shape and ensuring survival, particularly during division or stress response. The Streptococcus pneumoniae protein Spr1875 plays a role in response, both regulated VicRK two-component system (analogous to WalRK TCS found Firmicutes). Modular presents putative cell-wall binding module at N-terminus catalytic C-terminal (Spr1875MT3) connected long linker. Assays...