A5012674124- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Toxic Organic Pollutants Impact
- Toxoplasma gondii Research Studies
- Drug Transport and Resistance Mechanisms
- Carbohydrate Chemistry and Synthesis
- Estrogen and related hormone effects
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Antimicrobial Resistance in Staphylococcus
- Trypanosoma species research and implications
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
- Iron Metabolism and Disorders
- Antibiotic Resistance in Bacteria
- Retinoids in leukemia and cellular processes
- HIV Research and Treatment
- Biochemical and Molecular Research
- Protein Kinase Regulation and GTPase Signaling
- Escherichia coli research studies
Centre de Biologie Structurale
2020-2023
Institut de Recherche en Cancérologie de Montpellier
2022-2023
Inserm
2022-2023
Centre National de la Recherche Scientifique
2010-2022
Institut de Génomique Fonctionnelle
2022
Institut Universitaire de France
2022
Université de Montpellier
2022
Walter and Eliza Hall Institute of Medical Research
2015-2021
Laboratoire d'Enzymologie et Biochimie Structurales
2010-2011
University of Virginia
2009
shows a strict host tropism for reticulocytes. We identified transferrin receptor 1 (TfR1) as the
Abstract The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor that mediates broad spectrum of (patho)physiological processes in response to numerous substances including pollutants, natural products and metabolites. However, the scarcity structural data precludes understanding how AHR activated by such diverse compounds. Our 2.85 Å structure human indirubin-bound complex with chaperone Hsp90 co-chaperone XAP2, reported herein, reveals closed conformation dimer...
The study of antigenic targets naturally-acquired immunity is essential to identify and prioritize antigens for further functional characterization. We measured total IgG antibodies 38 P. vivax antigens, investigating their relationship with prospective risk malaria in a cohort 1–3 years old Papua New Guinean children. Using simulated annealing algorithms, the potential protective efficacy multiple antigen-combinations, antibody thresholds associated protection were investigated first time....
Background Major gaps in our understanding of Plasmodium vivax biology and the acquisition immunity to this parasite hinder vaccine development. P. merozoites exclusively invade reticulocytes, making proteins that mediate reticulocyte binding and/or invasion potential key or drug targets. While protein interactions are still poorly understood, Reticulocyte-Binding Protein family (PvRBP) is thought be involved restricted host-cell selectivity. Methodology/Principal findings We assessed...
Significance Chimpanzees, bonobos, and gorillas harbor close relatives of human Plasmodium vivax , but current knowledge these parasites is limited to a small number gene fragments derived almost exclusively from mitochondrial DNA. We compared nearly full-length genomes ape with global sample P. tested the function proteins believed be important for erythrocyte binding. The results showed that are 10-fold more diverse than exhibit no evidence species specificity, whereas represents...
Humans are chronically exposed to mixtures of xenobiotics referred as endocrine-disrupting chemicals (EDCs). A vast body literature links exposure these with increased incidences reproductive, metabolic, or neurological disorders. Moreover, recent data demonstrate that, when used in combination, have outcomes that cannot be predicted from their individual behavior. In its heterodimeric form the retinoid X receptor (RXR), pregnane (PXR) plays an essential role controlling mammalian xenobiotic...
Capsular polysaccharides are well-established virulence factors of pathogenic bacteria. Their biosynthesis and export regulated within the transmembrane polysaccharide assembly machinery by autophosphorylation atypical tyrosine-kinases, named BY-kinases. However, accurate functioning these tyrosine-kinases remains unknown. Here, we report crystal structure non-phosphorylated cytoplasmic domain tyrosine-kinase Wzc from Escherichia coli in complex with ADP showing that it forms a ring-shaped...
Significance Plasmodium vivax is responsible for the most widely distributed recurring human malaria infections whereas falciparum inflicts mortality and morbidity in populations. Malaria parasites enter our blood cells by making proteins that recognize bind to their cognate receptors on red cell surface. Our research describes, knowledge, first crystal structure of PvRBP2a, an erythrocyte-binding protein from P. vivax, which revealed a structural scaffold similar PfRh5, essential ....
Thailand is aiming to eliminate malaria by the year 2024. Plasmodium vivax has now become dominant species causing within country, and a high proportion of infections are asymptomatic. A better understanding antibody dynamics P. antigens in low-transmission setting, where acquired immune responses poorly characterized, will be pivotal for developing new strategies elimination, such as improved surveillance methods vaccines. The objective this study was characterize total IgG levels 11 key...
The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor belonging to the bHLH/PAS protein family and responding hundreds of natural chemical substances. It primarily involved in defense against insults bacterial infections or adaptive immune response, but also development pathological conditions ranging from inflammatory neoplastic disorders. Despite its prominent roles many (patho)physiological processes, lack high-resolution structural data has precluded for thirty...
ABSTRACT Members of the Plasmodium vivax reticulocyte binding protein (PvRBP) family are believed to mediate specific invasion reticulocytes by P. . In this study, we performed molecular characterization genes encoding members family. Through cDNA sequencing, constructed full-length gene models and verified that coding those pseudogenes. We also used quantitative PCR measure their in vivo transcript abundances clinical isolates. Like related ligands falciparum , Pvrbp expression levels vary...
Background The Plasmodium vivax Reticulocyte Binding Protein (PvRBP) family is involved in red blood cell recognition and members of this are potential targets for antibodies that may block P. invasion. To date, the acquisition immunity against PvRBPs low malaria transmission settings a broad age group exposed individuals has not been investigated. Methodology/Principal findings Total IgG antibody levels to six PvRBP (PvRBP1a, PvRBP1b, PvRBP2a, PvRBP2b, non-binding fragment PvRBP2c...
Bacterial UDP-sugar dehydrogenases are part of the biosynthesis pathway extracellular polysaccharides. These compounds act as important virulence factors by protecting cell from opsonophagocytosis and complement-mediated killing. In Staphylococcus aureus, protein Cap5O catalyzes oxidation UDP-N-acetyl-mannosamine to UDP-N-acetyl-mannosaminuronic acid. is crucial for production serotype 5 capsular polysaccharide that prevents interaction bacteria with both phagocytic nonphagocytic eukaryotic...
The DH-PH domain tandems of Dbl-homology guanine nucleotide exchange factors catalyze the GTP for GDP in Rho-family GTPases, and thus initiate a wide variety cellular signaling cascades. Although several crystal structures complexes with cognate, free Rho GTPases are known, they provide limited information about dynamics complex it is not clear how accurately represent solution. We used complementary combination nuclear magnetic resonance (NMR), small-angle X-ray scattering (SAXS),...
Abstract Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) P . invasion. First, observed reticulocytes was consistently reduced when CR1 surface expression through enzymatic cleavage, presence naturally low-CR1-expressing compared with high-CR1-expressing cells, addition soluble...
A particular class of tyrosine-kinases sharing no structural similarity with eukaryotic has been evidenced in a large array bacterial species. These are able to autophosphorylate on C-terminal tyrosine-rich motif. Their autophosphorylation shown play crucial role the biosynthesis or export capsular polysaccharide. The analysis first crystal structure staphylococcal tyrosine kinase CapB2 associated activating domain transmembrane modulator CapA1 had brought conclusive explanation for both and...
Summary Living organisms have developed protein sensors helping them to adapt their environment 1 . The aryl hydrocarbon receptor (AHR) is an emblematic member of this class proteins, and a ligand-dependent transcription factor that mediates broad spectrum (patho)physiological processes in response numerous substances including pollutants, natural products metabolites 2 However, the absence high-resolution structural data, molecular understanding how AHR activated by such diverse compounds...