The mechanism of high-level fluoroquinolone resistance was studied in strains Streptococcus pneumoniae, either selected vitro or isolated from clinical samples. By using DNA these high-level-resistant strains, low-level-resistant transformants (MIC pefloxacin, > = 32 micrograms/ml; MIC ciprofloxacin, 4 sparfloxacin, 0.50 micrograms/ml) were obtained at high frequencies (ca.10(-2)), while 64 16 to 8 only low (ca.10(-4)). This suggested that mutations least two unlinked genes necessary...

Background Previous studies have highlighted the increased risk of contracting COVID-19 for health-care workers and suggest that oral may carry greatest risk. Considering transmission route SARS-CoV-2 infection, a similar can be hypothesized other respiratory infections. However, no study has specifically assessed within dental profession. Methods An online survey was conducted population French professionals between April 1 29, 2020. Univariable multivariable logistic regression analyses...

In Brief Changes in the nerve's microenvironment and local inflammation resulting from peripheral nerve injury participate sensitization neuropathic pain development. Taking part these early changes, disruption of blood–nerve barrier (BNB) allows for infiltration immunocytes promotes neuroinflammation. However, molecular mechanisms engaged vascular endothelial cells (VEC) dysfunction BNB alterations remain unclear. vivo, permeability was assessed following chronic constriction (CCI) rat...

By using infrared and 19F nuclear magnetic resonance spectroscopies, we localized the binding site measured affinity of magnesium for six fluoroquinolones. It was proven that is situated between ketone carboxylate groups. We determined constants 1:1 Mg(2+)-drug complex in solution. Sparfloxacin pefloxacin, with (Ka) (10.1 +/- 0.6) x 10(2) M-1 (21 1) M-1, respectively, were least most bound, respectively. The trend affinities assayed fluoroquinolones correlated their antimicrobial activities...

Osseous dysplasia (OD) is the most common fibro-osseous lesion of jaw affecting periapical region. Early stages OD can resemble radiolucencies, thus mimicking radiological aspects an endodontic pathology. Such radiolucent lesions previously decayed or treated teeth are even more complex to interpret.The aim this paper report a case-series representative clinical situations describing features and illustrating diagnostic workup patients with florid osseous (FOD). Emphasis given implications...

Background: This study aimed to compare the corneal nerve structural abnormalities detected using in vivo confocal microscopy (IVCM) patients with neuropathic pain (NCP) secondary primary meibomian gland dysfunction (MGD) or autoimmune dry eye (AIDE). Methods: A two-stage retrospective nested case-control was conducted. First, data from either MGD AIDE were assessed, selecting only cases no (VAS = 0) severe ≥ 8). Ocular signs and symptoms of 238 selected compared between painful painless...

As a means of gaining information on the selectivity quinolone antibacterial agents, we examined their effect four topoisomerases, topoisomerases I and II purified from Escherichia coli calf thymus. The inhibition supercoiling relaxation activities was monitored by using classical gel electrophoresis assay. Eight quinolones were assayed enzymes. Gyrase much more sensitive to than other which can therefore be inhibited moderate concentrations certain quinolones. No good correlation observed...

Two associated resistance mechanisms were found in a nalidixic acid-susceptible (4 micrograms/ml) but fluoroquinolone-resistant (8 to 16 strain of Escherichia coli Q2 selected under norfloxacin therapy. As compared with the susceptible E. Q1 isolated before treatment, changes outer membrane proteins and lipopolysaccharides 1.5- 3-fold decrease uptake fluoroquinolones not acid. A 50% inhibition DNA synthesis toluene-permeabilized cells resistant required up 500-fold increased quantities...

Whereas neurovascular interactions in spinal neuropathic pain models have been well characterized, little attention has given to such orofacial models. This study investigated male Sprague-Dawley rats the vascular changes following chronic constriction injury (CCI) of infraorbital nerve (IoN), a broadly validated preclinical model pain. Following IoN-CCI, an early downregulation tight junction proteins Claudin-1 and Claudin-5 was observed within endoneurium perineurium, associated with...

A spontaneous Escherichia coli mutant, named Q3, resistant to nalidixic acid was obtained from a previously described clinical isolate of E. coli, Q2, fluoroquinolones but susceptible (E. Cambau, F. Bordon, Collatz, and L. Gutmann, Antimicrob. Agents Chemother. 37:1247-1252, 1993). Q3 harbored the mutation Asp82Gly in addition Gly81Asp Q2. The different mutations leading Gly81Asp, Asp82Gly, Gly81AspAsp82Gly were introduced into gyrA gene on plasmid pJSW102, resulting plasmids KNK453 (gyrAts)...

Quinolone-resistant Escherichia coli strains were isolated from poultry clinical samples in Saudi Arabia. The flocks had been treated with oxolinic acid or flumequine prophylaxis. measure of the uptake fluoroquinolones showed that none a reduced accumulation quinolones. result complementation wild-type E. gyrA gene, which restored fluoroquinolone susceptibility, and isolation DNA gyrase six isolates indicated resistant an altered gyrase. minimum effective dose ciprofloxacin for inhibition...

Abstract BACKGROUND Objective Structured Clinical Examinations (OSCEs) are amongst the most anxiety‐provoking competency assessment methods. An online serious game (OSCEGame) was developed and implemented within OSCE curriculum. This study aimed to evaluate usefulness of this on preparedness reducing OSCE‐related stress. METHODS A designed help dental students train for OSCEs. Two courses (4 stations each) were according year undergraduate training th 5 year), based 6 pre‐existing...

BackgroundBlood–nerve barrier disruption is pivotal in the development of neuroinflammation, peripheral sensitization, and neuropathic pain after nerve injury. Activation toll-like receptor 4 inactivation Sonic Hedgehog signaling pathways within endoneurial endothelial cells are key events, resulting infiltration harmful molecules immunocytes parenchyma. However, we showed a previous study that preemptive or sustained activation did not prevent local alterations observed following injury,...

Two coumarins, inhibitors of the B subunit DNA gyrase, and six quinolones, A subunit, were tested against Escherichia coli topoisomerase I-catalyzed relaxation. Coumarins had no effect, whereas quinolones enzyme. This inhibition was compared with that gyrase calf thymus I. The 50% inhibitory concentrations for E. I about one order magnitude higher than corresponding values but far lower known There a good relationship between two prokaryotic topoisomerases MICs coli, could be ranked in same...

L’évolution des thérapeutiques orthodontiques doit faire face à une demande croissante de traitements plus courts pour un même résultat final, en particulier chez les patients adultes. Ces dernières années, l’amélioration techniques orthodontiques, chirurgicales et anesthésiques chirurgie orthognathique ont permis développement considérable protocoles orthodontico-chirurgicaux la prise charge orthodontique le patient adulte. Les corticotomies alvéolaires font partie ces nouvelles techniques....

We determined the nucleotide sequence of a 6-kb DNA region harboring recF, orf192, gyrB, and gyrA genes from Mycobacterium smegmatis mc(2)155. The amino acid sequences deduced gyrB displayed 89 86% identity, respectively, with gyrase tuberculosis, 67 65% that Streptomyces coelicolor. An open reading frame encoding C-terminal M. RecF polypeptide was found upstream 57% identical to S. coelicolor protein. gene orf192 identified between recF 39% orf191 in recF-gyrB region. gyrase, which purified...