A5050601416- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Tissue Engineering and Regenerative Medicine
- Melanoma and MAPK Pathways
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Genomics, phytochemicals, and oxidative stress
- Chronic Myeloid Leukemia Treatments
- Telomeres, Telomerase, and Senescence
- Bladder and Urothelial Cancer Treatments
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Multiple Myeloma Research and Treatments
- melanin and skin pigmentation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- Genomics and Chromatin Dynamics
- FOXO transcription factor regulation
- Cell death mechanisms and regulation
- Cancer therapeutics and mechanisms
- Congenital gastrointestinal and neural anomalies
- Heat shock proteins research
- Cell Adhesion Molecules Research
Kaiser Permanente
2025
Charles River Laboratories (Germany)
2025
Cleveland Clinic Lerner College of Medicine
2020-2024
Charles River Laboratories (United States)
2024
Kaiser Permanente San Francisco Medical Center
2022
Roswell Park Comprehensive Cancer Center
2011-2021
Cleveland Clinic
2020
In normal human cells, oncogene-induced senescence (OIS) depends on induction of DNA damage response. Oxidative stress and hyperreplication genomic have been proposed as major causes in OIS cells. Here, we report that down-regulation deoxyribonucleoside pools is another endogenous source fibroblasts (NHFs) undergoing HRASG12V-induced senescence. NHF-HRASG12V cells underexpressed thymidylate synthase (TS) ribonucleotide reductase (RR), two enzymes required for the entire de novo...
Polyamine inhibition for cancer therapy is, conceptually, an attractive approach but has yet to meet success in the clinical setting. The aryl hydrocarbon receptor (AHR) is central transcriptional regulator of xenobiotic response. Our study revealed that AHR also positively regulates intracellular polyamine production via direct activation 2 genes, ODC1 and AZIN1, which are involved biosynthesis control, respectively. In patients with multiple myeloma (MM), levels were inversely correlated...
Non-muscle invasive bladder cancer (NMIBC) represents 70-80% patients with newly diagnosed cancer, and Bacillus Calmette-Guerin (BCG) remains a cornerstone treatment for intermediate high-risk NMIBC to prevent disease recurrence progression. However, many experience after induction BCG, posing significant challenges in the management of disease. We conducted single cell RNA sequencing on freshly collected samples, distinguishing between those naive BCG that recurred post-BCG treatment....
Continuous EEG (cEEG) has become a standard for monitoring critically ill patients, but it is resource-intensive with limited availability. The 2HELP2B seizure risk score can help stratify and aid in clinical decision making to optimize duration of monitoring. This study aimed incorporate the 2HELPS2B inform cEEG provide cost-effective care without compromising detection. We conducted quality improvement that targeted workflow stratification intensive units tertiary academic hospital....
Highlights•The carcinoma oncogene FOXQ1 suppresses invasion and metastasis in melanoma cells•FOXQ1 inversely regulates N-cadherin gene (CDH2) cells•β-Catenin levels determine the mode of regulation CDH2 invasionSummaryLineage-specific tumor progression by same transcription factor is understudied. We find that factor, an carcinomas, are decreased during progression. Moreover, contrast to epithelial-to-mesenchymal transition, invasion, cells. these lineage-specific functions largely depend on...
Resistance to the proteasome inhibitor bortezomib (BTZ) represents a major obstacle in treatment of multiple myeloma (MM). The contribution lipid metabolism resistance MM cells BTZ is mostly unknown. Here we report that levels fatty acid elongase 6 (ELOVL6) were lower from BTZ-nonresponsive vs BTZ-responsive patients and cultured selected for compared with parental counterparts. Accordingly, depletion ELOVL6 suppressed BTZ-induced endoplasmic reticulum (ER) stress cytotoxicity, whereas...
Introduction: The use of continuous electroencephalograms (cEEG) has increased over the last decade with guidelines recommending at least 24 hours monitoring to detect nonconvulsive seizures (NCS). However, recent studies suggest it may be unnecessary monitor low-risk patients beyond a few hours. Since cEEGs are resource-intensive, we sought optimize utilization by reducing nonvalue-added time on cEEG, applying seizure risk score, 2HELPS2B, based initial EEG findings and patient clinical...
Summary Tissue engineering offers a promising treatment strategy for ureteral strictures, but its success requires an in-depth understanding of the architecture, cellular heterogeneity, and signaling pathways underlying tissue regeneration. Here we define spatially map cell populations within human ureter using single-cell RNA sequencing, spatial gene expression, immunofluorescence approaches. We focused on stromal urothelial to enumerate distinct types composing inferred potential cell-cell...
ABSTRACT Alternative cleavage and polyadenylation (APA) is a gene regulatory mechanism used by cells under stress to upregulate proteostasis-promoting transcripts, but how achieve this remains poorly understood. Previously, we elucidated DNA methylation-regulated APA mechanism, in which body methylation enhances distal poly(A) isoform expression blocking CTCF binding chromatin loop formation at control regions. We hypothesized that one employ induce isoforms. At the DNAJB6 co-chaperone...