A5089090724- Nutrition, Genetics, and Disease
- Cancer Immunotherapy and Biomarkers
- Genetics and Physical Performance
- Cancer Genomics and Diagnostics
- Cancer Risks and Factors
- Immunotherapy and Immune Responses
- Breast Cancer Treatment Studies
- Epigenetics and DNA Methylation
- Global Cancer Incidence and Screening
- Bladder and Urothelial Cancer Treatments
- Evolution and Genetic Dynamics
- Genetic diversity and population structure
- Cancer survivorship and care
- BRCA gene mutations in cancer
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- HER2/EGFR in Cancer Research
- Gene expression and cancer classification
- Cancer, Stress, Anesthesia, and Immune Response
- Childhood Cancer Survivors' Quality of Life
- Colorectal Cancer Treatments and Studies
- FOXO transcription factor regulation
- Estrogen and related hormone effects
- Metabolism, Diabetes, and Cancer
- PI3K/AKT/mTOR signaling in cancer
Roswell Park Comprehensive Cancer Center
2015-2024
University of Florida
2019
Rutgers, The State University of New Jersey
2019
Rutgers Cancer Institute of New Jersey
2019
University at Buffalo, State University of New York
2001-2012
Buffalo State University
2011
Indiana University Bloomington
2006-2010
Indiana University
2008
The facilitates chromatin transcription (FACT) complex is involved in remodeling during transcription, replication, and DNA repair. FACT was previously considered to be ubiquitously expressed not associated with any disease. However, we discovered that the target of a class anticancer compounds normal cells adult mammalian tissues, except for undifferentiated stem-like cells. Here, show expression strongly poorly differentiated aggressive cancers low overall survival. In addition, found...
<h3>Background</h3> Immune checkpoint inhibitors (ICIs) have changed the clinical management of melanoma. However, not all patients respond, and current biomarkers including PD-L1 mutational burden show incomplete predictive performance. The validity utility complex been studied in <h3>Methods</h3> Cutaneous metastatic melanoma at eight institutions were evaluated for expression, CD8<sup>+</sup> T-cell infiltration pattern, burden, 394 immune transcript expression. IHC assessed association...
// Wa Zhang 1, 10 , Carter J. Barger 1 Kevin H. Eng 4 David Klinkebiel 2 Petra A. Link 3 Angela Omilian 5 Wiam Bshara Kunle Odunsi 6, 7, 8 Adam R. Karpf 9 Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE, USA Department Biochemistry and Molecular Biology, Pharmacology, Roswell Park Institute, Buffalo, NY, Biostatistics Bioinformatics, Pathology, 6 Gynecologic Oncology, 7 Immunology, Center Immunotherapy, Fred Pamela Buffett Current address: Wilmer Eye...
Blacks tend to have a stronger inflammatory immune response than Whites. We hypothesized that racial differences in host immunity also manifest the tumor microenvironment, constituting part of distinct aggressive biology underlying higher mortality Black women.Pathological and gene expression profiling approaches were used for characterizing infiltrating cells breast microenvironment from 1315 patients Women's Circle Health Study. Racial phenotypes compared, with results validated publicly...
Despite the enormous theoretical attention given to evolutionary consequences of sexual reproduction, validity key assumptions on which theory depends rarely has been evaluated. It is often argued that a reduced ability purge deleterious mutations condemns asexual lineages an early extinction. However, most well characterized fail exhibit high levels neutral allelic divergence expected in absence recombination. With purely descriptive data, it difficult evaluate whether this pattern...
The Hippo signaling pathway regulates cellular proliferation and survival, thus exerting profound effects on normal cell fate tumorigenesis. We previously showed that the pivotal effector of this pathway, YAP, is amplified in tumors promotes epithelial-to-mesenchymal transition (EMT) malignant transformation. Here, we report overexpression TAZ, a paralog human mammary epithelial cells EMT and, particular, some invasive structures 3D cultures. TAZ also leads to migration anchorage-independent...
Daphnia pulex is quickly becoming an attractive model species in the field of ecological genomics due to recent release its complete genome sequence, a wide variety new genomic resources, and rich history data. Sequences mitochondrial NADH dehydrogenase subunit 5 cytochrome c oxidase 1 genes were used assess global phylogeography this species, further elucidate phylogenetic relationship other members complex. Using both newly acquired previously published data, we analyzed 398 individuals...
Significance Genetic alterations are frequently observed in bladder cancer. In this study, we demonstrate that tumors can be classified into two different types based on the spectrum of genetic diversity they confer. one class tumors, tumor protein p53 mutations and a large number single-nucleotide structural variants. Another characteristic group was chromosome shattering, known as chromothripsis, mutational heterogeneity. The other did not show these profound aberrations, but found novel...
We have developed a next-generation sequencing assay to quantify biomarkers of the host immune response in formalin-fixed, paraffin-embedded (FFPE) tumor specimens. This aims provide clinicians with comprehensive characterization immunologic microenvironment as guide for therapeutic decisions on patients solid tumors. The relies RNA-sequencing (seq) semiquantitatively measure levels 43 transcripts related anticancer responses and 11 that reflect relative abundance tumor-infiltrating...
Abstract Background Anti-tumorigenic vs pro-tumorigenic roles of estrogen receptor-beta (ESR2) in breast cancer remain unsettled. We investigated the potential TP53 status to be a determinant bi-faceted role ESR2 and associated therapeutic implications for triple negative (TNBC). Methods ESR2-TP53 interaction was analyzed with multiple assays including situ proximity ligation assay. Transcriptional effects on TP53-target genes cell proliferation response knocking down or overexpressing were...
Duplication and divergence of genes genetic networks is hypothesized to be a major driver the evolution complexity novel features. Here, we examine history in context eye by using new approaches understand patterns gene duplication during metazoan genomes. We hypothesize that 1) involved development phototransduction have duplicated are retained at higher rates animal clades possess more distinct types optical design; 2) with functional relationships were lost together, thereby preserving...
Melanoma is one of the most aggressive types human cancers, and mechanisms underlying melanoma invasive phenotype are not completely understood. Here, we report that expression guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis purine nucleotides, was downregulated stages melanoma. Loss- gain-of-function experiments revealed GMPR downregulates amounts several GTP-bound (active) Rho-GTPases suppresses ability cells to form invadopodia, degrade extracellular...
The reprogramming of lipid metabolism is a hallmark many cancers that has been shown to promote breast cancer progression. While several signatures associated with aggressiveness have identified, comprehensive lipidomic analysis specifically targeting the triple-negative subtype (TNBC) may be required identify novel biomarkers and therapeutic targets for this most aggressive still lacks effective therapies. In current study, our global LC-MS-based lipidomics platform was able measure 684...
Current criteria for identifying cancer patients suitable immunotherapy with immune checkpoint blockers (ICBs) are subjective and prone to misinterpretation, as they mainly rely on the visual assessment of CD274 (best known PD-L1) expression levels by immunohistochemistry (IHC). To address this issue, we developed a RNA sequencing (RNAseq)-based approach that specifically measures abundance transcripts in formalin-fixed paraffin embedded (FFPE) specimens. Besides exhibiting superior...
Abstract Background African American/Black women with breast cancer have poorer survival than White women, and this disparity persists even after adjusting for non-biological factors. Differences in tumor immune biology been reported between Black the milieu could potentially drive racial differences etiology outcome. Methods We examined association of CD8 + cytotoxic T cells clinical-pathological variables Women’s Circle Health Study (WCHS) population predominantly patients. evaluated 688...
Racial disparities in breast cancer (BC) survival arise from multilevel causes, which may exert influence at different stages of BC progression. Clarifying the importance genetic and social factors could help prioritize interventions.
Importance It is unclear whether breast cancer (BC) with low ERBB2 expression (ERBB2-low) a distinct clinical, pathological, and epidemiological entity from BC classified as no (ERBB2-negative). Objective To evaluate the epidemiologic features of ERBB2-low compared ERBB2-negative in large population study. Design, Setting, Participants This cohort study was conducted part Pathways Study, prospective, racially ethnically diverse women enrolled between 2006 2013 Kaiser Permanente Northern...
Objective Having children reduces risk of breast cancer overall, but parity without breastfeeding, more prevalent among black women, increases poor-prognosis oestrogen receptor negative (ER−) cancer. We investigated if relationships between parity, breastfeeding and ER subtypes result from epigenetic programming, potentially steering progenitor cells to a basal-like phenotype. Methods analysis The Illumina MethylationEPIC platform was used assess genome-wide methylation in formalin-fixed,...
Abstract Background: Tumor-infiltrating lymphocytes (TILs) within the tumor and its surrounding microenvironment reflect an active immune response are regarded as a prognostic marker for breast cancer. However, host factors that affect TILs remain less explored. The study investigated association between lifetime hormone exposure TILs. Methods: included newly diagnosed invasive cancer cases in Women's Circle of Health Study. Lifetime endogenous (LHEendo) was defined sum reproductive years...
Abstract Background: There are well-documented racial and ethnic disparities in breast cancer, with Black women having the highest mortality Asian lowest mortality. While reasons for this disparity multi-factorial, differences tumor biology microenvironment may contribute. Over past two decades, pathologic tumor-infiltrating lymphocytes (TILs) have emerged as a histologic biomarker of prognosis, where higher TIL levels associated better outcomes overall survival, predictive cancer treatment....
The nuclear large subunit (LSU) rRNA gene is a rich source of phylogenetic characters because its size, mosaic slowly and rapidly evolving regions, complex secondary structure variation. Nevertheless, many studies have indicated that inconsistency, bias, gene-specific error (e.g., within-individual family variation, cryptic sequence simplicity, coevolution) can complicate animal phylogenies based on LSU rDNA sequences. However, most these sampled small fragments from expansion segments—among...
To what extent steroid hormones contribute to lung cancer in male and female never smokers is unclear. We examined expression of hormone receptors tumors by sex smoking. Patients with primary non–small cell were recruited into an Intergroup study the United States Canada, led SWOG (S0424). Tumors from 813 cases (450 women 363 men) assayed using immunohistochemistry for estrogen receptor (ER)–α, ER-β, progesterone (PR), human epidermal growth factor 2 (HER2). Linear regression was used...
Abstract Background Tumor-associated macrophages (TAMs) are a prominent immune subpopulation in the tumor microenvironment that could potentially serve as therapeutic targets for breast cancer. Thus, it is important to characterize this cell population across different subtypes including patterns of association with demographic and prognostic factors, cancer outcomes. Methods We investigated CD163 + relation clinicopathologic variables outcomes Women’s Circle Health Study Follow-up...
Here, we report 2 novel intron gains segregating in populations of Daphnia pulex endemic to Oregon. These introns do not have an obvious source and are present any D. outside Oregon, other species that examined, or organism for which sequence data available. Furthermore, the both found same gene, a Rab GTPase (rab4), they appear differ their insertion site by one base pair, providing some support proto-splice hypothesis. The rarity intron-gain polymorphisms is questioned as discovered events...