A5081260368- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Enzyme Structure and Function
- Heavy Metal Exposure and Toxicity
- Adipose Tissue and Metabolism
- Biochemical and Structural Characterization
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Heavy metals in environment
- Selenium in Biological Systems
- Analytical Chemistry and Chromatography
- Peptidase Inhibition and Analysis
- Escherichia coli research studies
- Cardiac Structural Anomalies and Repair
- Arsenic contamination and mitigation
- Radioactivity and Radon Measurements
- Polish Historical and Cultural Studies
- Neuropeptides and Animal Physiology
- Geochemistry and Geologic Mapping
- Antibiotic Resistance in Bacteria
- Pancreatic function and diabetes
- Bacterial biofilms and quorum sensing
VIB-UGent Center for Inflammation Research
2022-2023
Ghent University
2022-2023
KU Leuven
2016-2022
Rega Institute for Medical Research
2016-2022
University of Crete
2010-2014
Hormone absence or inactivity is common in congenital disease, but hormone antagonism remains controversial. Here, we characterize two novel homozygous leptin variants that yielded antagonistic proteins unrelated children with intense hyperphagia, severe obesity, and high circulating levels of leptin. Both bind to the receptor trigger marginal, if any, signaling. In presence nonvariant leptin, act as competitive antagonists. Thus, treatment recombinant was initiated at doses, which were...
Secretory proteins are only temporary cytoplasmic residents. They typically synthesized as preproteins, carrying signal peptides N-terminally fused to their mature domains. In bacteria secretion largely occurs posttranslationally through the membrane-embedded SecA-SecYEG translocase. Upon crossing plasma membrane, cleaved off and domains reach destinations fold. Targeting translocase is mediated by peptides. The role of in targeting unclear. We now reveal that harbor own independent signals...
Antimony concentrations up to a factor of 2.7 above the EU limit for drinking water were found in commercial juices and may either be leached from packaging material or introduced during manufacturing, pointing out need further research on area.
Prolyl oligopeptidase (PREP) is conserved in many organisms across life. It involved numerous processes including brain function and neuropathology, that require more than its strict proteolytic role. consists of a seven-bladed β-propeller juxtaposed to catalytic α/β-hydrolase domain. The conformational dynamics PREP domain motions the gating mechanism allows substrate accessibility remain elusive. Here we used Hydrogen Deuterium eXchange Mass Spectrometry (HDX-MS) derive first near-residue...
Cellular proteomes are distributed in multiple compartments: on DNA, ribosomes, and inside membranes, or they become secreted. Structural properties that allow polypeptides to occupy subcellular niches, particularly after crossing remain unclear. We compared intrinsic extrinsic features cytoplasmic secreted of the Escherichia coli K-12 proteome. between cytoplasmome secretome sharply distinct, such a signal peptide-agnostic machine learning tool distinguishes from proteins with 95.5%...
Novel biophysical tools allow the structural dynamics of proteins and regulation such by binding partners to be explored in unprecedented detail. Although this has provided critical insights into protein function, means which direct evolution remain poorly understood. Here, we investigated how with a bilobed structure, composed two related domains from periplasmic-binding protein-like II domain family, have undergone divergent evolution, leading adaptation their dynamics. We performed...
In this study, we report on the development of a novel nebulizer configuration for sonic-spray ionization (SSI) mass spectrometry (MS), more specifically version SSI that is referred to as Venturi easy ambient (V-EASI) MS. The developed based commercially available pneumatic glass has been used extensively aerosol formation in atomic spectrometry. present was modified order achieve efficient V-EASI-MS operation. Upon evaluating system, it demonstrated offers some distinct advantages analysis...
Abstract Novel biophysical tools allow the structural dynamics of proteins, and regulation such by binding partners, to be explored in unprecedented detail. Although this has provided critical insights into protein function, means which direct evolution remains poorly understood. Here, we investigated how proteins with a bilobed structure, composed two related domains from type-II periplasmic domain family, have undergone divergent leading modification their function. We performed analysis...
Abstract Secretory preproteins of the Sec pathway bear signal peptides and are targeted post-translationally to cross plasma membrane or ER through translocases. After translocation peptide cleavage, mature domains fold native states in bacterial periplasm after further trafficking. During cytoplasmic transit, must remain non-folded for translocase recognition translocation. Here, we sought structural basis delayed folding mechanism how this is regulated by peptides. To address this,...
Abstract The adipokine Leptin activates its type I cytokine receptor (LEP-R) in the hypothalamus to regulate body weight and exerts additional pleiotropic functions immunity, fertility, cancer. However, structure mechanism of Leptin-mediated LEP-R assemblies has remained unclear. Here, we show that Leptin:LEP-R adopt an unprecedented within family featuring 3:3 stoichiometry. We validate Leptin-induced trimerization plasma membrane living cells via multicolor single molecule microscopy. In...
Brief summary: The authors provide the first description of antagonistic hormone mutations as cause congenital disease in humans. paper describes detailed characterizations two novel, leptin underlying a formerly unrecognized form dysfunction and delineate challenges these pose to diagnosis therapy disease.
The authors appreciate Vasami's contribution to the debate on Sb in foodstuff but disagree that their communication contains misinterpretations and inaccuracies.