Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, is overexpressed in prostate cancer, but the mechanism by which MIF exerts effects on tumor cells remains undetermined. interacts with its identified membrane receptor, CD74, association CD44, resulting ERK 1/2 activation. Therefore, we hypothesized that increased expression or surface localization of CD74 and overexpression cancer regulated cell viability. Prostate lines (LNCaP DU-145) had gene protein levels compared...

Male Sprague-Dawley rats, with their pelvic and hypogastric nerves transected, were infected pseudorabies virus (PRV) injected into the external urethral sphincter. Animals sacrificed at 2, 2.5, 3, 4 days postinfection. Spinal cord brain tissue sectioned processed by immunohistochemical techniques antisera against PRV choline acetyl transferase (CAT). At 2 postinfection, virus-labeled neurons found in ventrolateral divisions of Onuf's nucleus dorsal gray commissure (DGC). progressively later...

Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful preserving effectiveness antibiotics and preventing emergence bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit several decades ago. Here we investigate mechanism inhibition find primary effect on occlusion conjugative pilus by phage particles. This interaction mediated primarily...

The importance of various inflammatory cytokines in maintaining tumor cell growth and viability is well established. Increased expression the proinflammatory cytokine macrophage migration inhibitory factor (MIF) has previously been associated with types adenocarcinoma.

Abstract Following the transection of one pelvic nerve and both hypogastric nerves, urinary bladder male Sprague‐Dawley rats was injected with pseudorabies virus (PRV; Bartha strain). The central stump transected labelled fast blue (FB), were maintained for 2, 2. 5, 3 days following viral infection. Tissue processed antisera against PRV choline acetyltransferase (CAT). In L6‐S1 spinal cord, neurons in ipsilateral intermediolateral area (IML) after 2 days. After 5 days, also found dorsal gray...

Macrophage migration inhibitory factor (MIF) is a cytokine associated with prostate cancer, based on histologic evidence and circulating (serum) levels. Recent studies from another laboratory failed to document these results. This study's aims were extend confirm our previous data, as well define possible mechanisms for the discrepant Additional examine MIF expression, location of MIF's receptor, CD74, in human prostatic adenocarcinoma compared matched benign prostate.MIF amounts determined...

Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine involved in cystitis and non-cognate ligand of the chemokine receptor CXCR4 vitro. We studied whether CXCR4-MIF associations occur rat bladder effect experimental cystitis.Twenty male rats received saline or cyclophosphamide (40 mg/kg; i.p.; every 3(rd) day) to induce persistent cystitis. After eight days, urine was collected bladders excised under anesthesia. Bladder co-localization were examined with...

Introduction Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is constitutively expressed in urothelial cells that also express protease-activated receptors (PAR). Urothelial PAR1 were shown to mediate bladder inflammation. We showed and PAR4 activator, thrombin, mediates MIF release. hypothesized stimulation of or elicits release acts on the urothelium inflammation pain. Thus, we examined effect activation specific PAR release, pain, micturition histological...

Abstract A double‐peroxidase procedure was used to study the ultrastructural relationship between terminals and fibers containing three putative neurotranasmitters retrogradely identified sympathetic preganglionic neurons (SPNS) located in intermediolateral cell column (IML) of rat. SPNS with axons cervical trunk were labeled horseradish peroxidase. In addition, substance P, enkejphalin, serotonin detected using immunohistochemistry. Sections both immunoreactive processes processed for...

Abstract Background The receptor for the cardiac hormone atrial natriuretic peptide (ANP), A (NPRA), is expressed in cancer cells, and peptides have been implicated cancers. However, direct role of NPRA signaling prostate remains unclear. Results expression was examined by western blotting, RT-PCR immunohistochemistry. downregulated transfection siRNA, shRNA inhibitor (iNPRA). Antitumor efficacy iNPRA tested mice using a TRAMP-C1 xenograft. Here, we demonstrated that abundantly on...

Pain is the significant presenting symptom in Interstitial Cystitis/Painful Bladder Syndrome (IC/PBS). Activation of urothelial protease activated receptor 4 (PAR4) causes pain through release macrophage migration inhibitory factor (MIF). High Mobility Group Box-1 (HMGB1), a chromatin-binding protein, mediates bladder (but not inflammation) an experimental model (cyclophosphamide) cystitis. To determine if PAR4-induced hypersensitivity depends on HMGB1 downstream, we tested whether: 1) PAR4...

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Apr 2004SUBSTANCE P INDUCED RELEASE OF MACROPHAGE MIGRATION INHIBITORY FACTOR FROM RAT BLADDER EPITHELIUM KATHERINE L. MEYER-SIEGLER and PEDRO VERA MEYER-SIEGLERKATHERINE More articles by this author VERAPEDRO View All Author Informationhttps://doi.org/10.1097/01.ju.0000115883.49365.1aAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Substance (SP),...

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine found in epithelial cells as preformed stores, such that MIF release can activate innate immune responses. Our identification of stores the urothelium suggests may function bladder's initial response to infectious stimuli, lipopolysaccharide (LPS). To test this hypothesis, we observed changes MIF, cyclooxygenase-2 (COX-2) and c-fos bladder, L6-S1 spinal cord, dorsal root ganglion (DRG), major pelvic (MPG) prostate...

MIF is a proinflammatory cytokine present in preformed stores human urothelium. In animal models of bladder inflammation, including bacterial cystitis, up-regulated the and released from as high molecular weight complex. We compared urine amounts patients with UTI to that without UTI, we examined identified MIF-protein complexes urine.Using enzyme-linked immunosorbent assay levels 14 16 controls no UTI. Western blotting under native, denaturing reducing conditions was done examine found...

The objective of this study was to determine if macrophage migration inhibitory factor (MIF) is upregulated in the bladder during persistent cystitis. MIF a pro-inflammatory cytokine found pre-formed urothelium. Previous findings showed that acute inflammation increased release into lumen while upregulating and CD74 (MIF receptor) bladder. Because effects cystitis on are not known, changes were examined after short-term (1-day) or (8-day) cyclophosphamide (CYP)-induced inflammation....