A5068519388- Birth, Development, and Health
- Anesthesia and Neurotoxicity Research
- Effects and risks of endocrine disrupting chemicals
- Neuroscience and Neuropharmacology Research
- Prenatal Substance Exposure Effects
- Carcinogens and Genotoxicity Assessment
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Folate and B Vitamins Research
- Adipose Tissue and Metabolism
- Neuroendocrine regulation and behavior
- Toxic Organic Pollutants Impact
- Pharmacological Effects and Toxicity Studies
- Stress Responses and Cortisol
- Heavy Metal Exposure and Toxicity
- Renal and related cancers
- RNA Interference and Gene Delivery
- Retinoids in leukemia and cellular processes
- Reproductive System and Pregnancy
- Inhalation and Respiratory Drug Delivery
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Animal testing and alternatives
National Institute of Health Sciences
2019-2024
National Institute of Health Sciences
2021-2022
Food and Drug Safety Center
2009-2019
Showa University
2005-2019
Kyoto University
2009
Central Institute for Experimental Animals
2009
Hamamatsu University School of Medicine
2009
Saitama International Medical Center
2009
Indiana University School of Medicine
2005
Indiana University – Purdue University Indianapolis
2005
Abstract Prenatal exposure to chemicals is well known induce developmental abnormalities in the central nervous system of children. Developmental neurotoxicity (DNT) tests are important identify neurotoxic agents and prevent neurodevelopmental disorders. We have investigated DNT, focusing on fetal brain shortly after chemical exposure. To demonstrate a usefulness study DNT tests, we assessed rat valproate‐induced autism model. Rats were treated with sodium valproate (VPA, 800 mg/kg) orally...
Background: Alcohol exposure during the fetal stage generates variable severity in different organs, as seen alcohol syndrome and effect. Whether genetic factors or conditions of influence susceptibility to alcohol-related developmental impairment remains a question. Methods: To investigate contribution genotype alcohol-induced toxicity development beyond confounding maternal variables exposures, authors tested effect under definitive concentration using whole embryonic culture two inbred...
A longevity gene product, Sir2 (silent information regulator 2) is a NAD-dependent histone deacetylase involved in yeasts, worms and flies. The mammalian homolog of Sir2, SIRT1(sirtuin 1), has been shown to play important roles related anti-aging effects (regulating apoptosis, stress tolerance, insulin resistance, fat metabolism). Recently, SIRT1 expression demonstrated occur at as early embryonic day 10.5 mice. during developing period may be the mechanism developmental origins adult...
Historical control data from prenatal developmental toxicity studies in rats have been used to evaluate whether toxicology outcomes were induced by exposure a chemical or within the range of spontaneous variation. These are also important for monitoring animal characteristics. As follow‐up historical 1998 2010, this study analyzed performed 2011 2015. Data collected 24 Japanese laboratories, including 15 pharmaceutical and companies nine contract research organizations, Sprague‐Dawley...
The purpose of the present study was to collect background data from repeated dose toxicity studies in Wistar Hannover [Crl:WI(Han)] (hereafter Han) rats with dosing periods 4, 13 and 26 weeks four safety research facilities pharmaceutical companies contract organizations participating International Genetic Standardization (IGS) rat forum supported by Charles River Laboratories Japan, Inc. Han were compared those Sprague Dawley Crl:CD(SD) rats. In addition, effects restricted feeding SD also...
Recent findings have revealed that extracellular vesicles (EVs) are secreted from cells and circulate in the blood. EVs classified as exosomes (40-100 nm), microvesicles (50-1,000 nm) or apoptotic bodies (500-2,000 nm). contain mRNAs, microRNAs, DNAs ability to transfer them cell cell. Recently, especially humans, diagnostic accuracy of tumor type-specific EV-associated miRNAs biomarkers has been found be more than 90 %. In addition, microRNAs contained blood being identified specific...
ABSTRACT The majority of prior developmental neurotoxicity studies focused on postnatal subjects rather than the fetus. In present paper, we demonstrate use histological examination fetal rat (embryonic day 16.5) brain serial sections, employing Nissl staining and microtubule‐associated protein 2 (MAP2) immunohistochemistry, in evaluating a chemical‐induced neurodevelopmental disorder. Since prenatal treatment with 5‐bromo‐2′‐deoxyuridine (BrdU) is known to induce behavioral abnormalities...
Human epidemiological evidence has led scientists to theorize that undernutrition during gestation is an important early origin of adult diseases. Animal models have successfully demonstrated maternal diet could contribute some Undernutrition perceived harmful in pregnant women, whereas calorie restriction a strategy proven extend healthy and maximum lifespan adult. This diagrammatically opposite effect nutritional condition might provide us with hints search for genes underlying health...
Low birthweight resulting from a non-optimal fetal environment is correlated epidemiologically to higher risk of adult diseases, and which has also been demonstrated using animal models for maternal undernutrition. In this study, we subjected pregnant mice 50% food restriction (FR), profiled gene expression promoter DNA methylation genome-wide the livers. The fact that effect opposite between before after birth encouraged us hunt genes are expressed oppositely calorie (CR) Among regulated...
Inhaled ciclesonide (CIC), a corticosteroid used to treat asthma that is also being investigated for the treatment of corona virus disease 2019, hydrolyzes desisobutyryl-ciclesonide (des-CIC) followed by reversible esterification when exposed fatty acids in lungs. While previous studies have described distribution and metabolism compounds after inhalation, spatial localization lungs remains unclear. We visualized two-dimensional CIC its metabolites rat administration single dose aerosol...
The embryonic stem cell test (EST) is an in vitro assay that has been developed to assess the embryotoxic potential of chemicals and biomaterials. It based on capacity mouse line D3 (ES-D3 cells) differentiate into contracting myocardial cells. However, EST method cannot fully embryotoxicity mercury vapor. We tried apply rotary culture bottle containing vapor medium was slowly turned cells come direct contact with gaseous phase At exposure concentrations tested this study, no pulsations were...
Extracellular vesicles (EVs) are particles released not only from blood cells but also various organs. EVs, which lipid bilayer vesicles, contain proteins, DNAs, and RNAs. The RNA proteins within EVs display cell-specific characteristics. derived tumor identified as biomarkers with diagnostic accuracy exceeding 90% for early cancer detection. Furthermore, EV in serum has serves a biomarker toxicity. have been found body fluids, including saliva, tears, urine, amniotic fluid. In this study,...
Rats exposed prenatally to 5-bromo-2'-deoxyuridine (BrdU-rats) display hyperlocomotive activity, making them a possibly useful animal model for the study of attention deficit hyperactivity disorder (ADHD). Using this model, we investigated dopamine transporter (DAT) density and behavioral outcomes in BrdU-rats, some which were also administered methylphenidate, psychostimulant that is widely used treatment ADHD. Pregnant rats BrdU from gestational day 9 through 15. In male offspring, DAT...
This study serves to further define the capabilities of whole embryo culture system using well-known teratogen, 5-fluorouracil (5-FU), an antineoplastic agent. An initial in vivo was performed whereby pregnant rats were injected intraperitoneally with 10-30 mg/kg 5-FU on day 9 gestation. On 20 gestation, effects this drug growth and development embryos evaluated. The number externally malformed fetuses increased a dose-related manner, most common defect micro-/anophthalmos dams treated 5-FU....