A5082965048- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- Ion channel regulation and function
- Genetics, Aging, and Longevity in Model Organisms
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Neuroendocrine regulation and behavior
- Cardiac electrophysiology and arrhythmias
- Heart Rate Variability and Autonomic Control
- Neurobiology and Insect Physiology Research
- Temporomandibular Joint Disorders
- Neuroscience of respiration and sleep
- Maternal Mental Health During Pregnancy and Postpartum
- Mental Health and Patient Involvement
- Connexins and lens biology
- Pluripotent Stem Cells Research
- Pregnancy and Medication Impact
- Congenital heart defects research
- Urticaria and Related Conditions
- Regulation of Appetite and Obesity
- Resilience and Mental Health
- CRISPR and Genetic Engineering
- Tryptophan and brain disorders
Washington University in St. Louis
2013-2023
University of Maryland, Baltimore
2005-2010
University of Pittsburgh
2008
Rutgers, The State University of New Jersey
2008
University of Dental Medicine
2007
The present study was designed to test the hypothesis that estrogen exacerbates inflammation of temporomandibular joint (TMJ). Evans blue dye used quantify plasma extravasation (PE) around rat TMJ. In an initial set experiments, TMJ PE compared in naïve intact male and female rats, as well both groups after complete Freund’s adjuvant (CFA)-induced contrast our hypothesis, significantly greater CFA-inflamed rats than females. To determine whether these differences were due gonadal hormones,...
The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as consequence fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie in pulpal afferents, we used single-cell PCR approach to screen retrogradely labeled afferents. presence mRNA encoding BNC-1, ASIC3, TRPV4, TRPA1, the α, β, and γ subunits ENaC, two pore K + channels (TREK1, TREK2) TRAAK were screened...
The formation of heteromeric tetramers is a common feature voltage-gated potassium (Kv) channels. This results in the generation variety tetrameric Kv channels that exhibit distinct biophysical and biochemical characteristics. Kv2 delayed rectifier are, however, unique exceptions. It has been previously shown mammalian Kv2.1 Kv2.2 are localized domains neuronal membranes not capable forming with each other (Hwang, P. M., Glatt, C. E., Bredt, D. S., Yellen, G., Snyder, S. H. (1992)...
Considerable evidence suggests that day–night rhythms in the functional expression of subthreshold potassium (K+) channels regulate daily oscillations spontaneous firing rates neurons suprachiasmatic nucleus (SCN), master circadian pacemaker mammals. The K+ conductance(s) driving these repetitive SCN neurons, however, have not been identified. To test hypothesis Kv12.1/Kv12.2-encoded play a role, we obtained current-clamp recordings from slices prepared adult mice harboring targeted...
The Kv2 voltage-gated potassium channels, Kv2.1 and Kv2.2, are important regulators of neuronal excitability in mammalian brain. It has been shown that channels expressed virtually all neurons the However, cellular localization Kv2.2 not fully elucidated. In this article we report is highly a subset magnocellular preoptic nucleus (MCPO) horizontal limb diagonal band Broca (HDB) basal forebrain complex, which areas implicated regulation cortical activity sleep/wake cycle. MCPO HDB contain...
The suprachiasmatic nucleus (SCN) regulates daily rhythms in physiology and behavior. Previous studies suggest a critical role for neurons expressing vasoactive intestinal peptide (VIP) coordinating rhythmicity synchronization the SCN. Here we examined firing properties of VIP-expressing SCN acute brain slices. Active passive membrane were measured VIP non-VIP during day at night. Current-clamp recordings revealed that both spontaneously active, with higher rates than Average frequencies,...
Abstract Rapidly activating and inactivating A-type K + currents (I A ) encoded by Kv4.2 Kv4.3 pore-forming (α) subunits of the Kv4 subfamily are key regulators neuronal excitability. Previous studies have suggested a role for Kv4.1 α-subunits in regulating firing properties mouse suprachiasmatic nucleus (SCN) neurons. To test this, we utilized an RNA-interference strategy to knockdown Kv4.1, acutely selectively, SCN. Current-clamp recordings revealed that vivo significantly ( p < 0.0001)...
Neurons in the suprachiasmatic nucleus (SCN), master circadian pacemaker mammals, display daily rhythms electrical activity with more depolarized resting potentials and higher firing rates during day than at night. Although these variations properties of SCN neurons are required for physiology behavior, mechanisms linking changes neuronal excitability to molecular clock not known. Recently, we reported that mice deficient either Kcna4 ( Kv1.4 -/- ) or Kcnd2 Kv4.2 ; but Kcnd3, Kv4.3 ),...
The basal forebrain (BF) has been implicated as an important brain region that regulates the sleep-wake cycle of animals. Gamma-aminobutyric acidergic (GABAergic) neurons are most predominant neuronal population within this region. However, due to lack specific molecular tools, roles BF GABAergic have not fully elucidated. Previously, we found high expression levels Kv2.2 voltage-gated potassium channel on approximately 60% in magnocellular preoptic area and horizontal limb diagonal band...
Neurons in the suprachiasmatic nucleus (SCN) generate circadian changes rates of spontaneous action potential firing that regulate and synchronize daily rhythms physiology behavior. Considerable evidence suggests repetitive (higher during day than at night) SCN neurons are mediated by subthreshold potassium (K
Considerable evidence suggests that day-night rhythms in the functional expression of subthreshold potassium (K
Summary The mammalian suprachiasmatic nucleus (SCN) functions as a master circadian pacemaker, integrating environmental input to align physiological and behavioral rhythms local time cues. Approximately 10% of SCN neurons express vasoactive intestinal polypeptide (VIP); however, it is unknown how firing activity VIP releases entrain rhythms. To identify physiologically relevant patterns, we optically tagged characterized spontaneous over three days. had in rate exhibited two classes...