A5100394689- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Digital Holography and Microscopy
- RNA Research and Splicing
- Leech Biology and Applications
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Optical measurement and interference techniques
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Advanced X-ray Imaging Techniques
- Electrochemical Analysis and Applications
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- Image Processing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Electrochemical sensors and biosensors
- DNA and Nucleic Acid Chemistry
- Advanced Fluorescence Microscopy Techniques
- Identification and Quantification in Food
- SARS-CoV-2 and COVID-19 Research
Chongqing University
2015-2025
Nankai University
2023-2025
State Key Laboratory of Mechanical Transmission
2025
Aerospace Information Research Institute
2025
Xidian University
2025
Central South University of Forestry and Technology
2025
Central South University
2025
Nanjing Agricultural University
2015-2024
Xi'an University of Science and Technology
2024
Jining Medical University
2024
Don′t FRET when I GO: Sensitive and selective rotavirus detection is achieved by using the photoluminescence of a graphene oxide (GO) array. The target cell was captured rotavirus-specific antibody immobilized on GO array, binding event monitored observing fluorescence quenching that results from resonance energy transfer (FRET) between gold nanoparticles linked to antibodies (see picture).
DEAD-box proteins, the largest helicase family, catalyze ATP-dependent remodeling of RNA-protein complexes and unwinding RNA duplexes. Because proteins hydrolyze ATP in an RNA-dependent fashion, energy provided by hydrolysis is commonly assumed to drive energetically unfavorable duplex unwinding. Here, we show efficient stable duplexes several presence nonhydrolyzable analog ADP-beryllium fluoride. Another analog, ADP-aluminum fluoride, does not promote The findings that from dispensable for...
Significance A ribosomal frameshift occurs when the ribosome slips by one or more nucleotides on messenger RNA (mRNA) during translation. Programmed frameshifting produces than protein from a single mRNA and is tightly regulated sequence structure. Using single-molecule fluorescence resonance energy transfer, we studied effects of stimulatory structure conformational dynamics translocation rate. Our results show that shifts equilibrium complexes away conformations favor process, resulting in...
Due to its low growth rate and fastidious nature, Mycobacterium tuberculosis is difficult identify. Its rapid sensitive detection is, however, critical for the control of tuberculosis. Molecular biology, more recently electrochemical technology, have been exploited this pathogen. In present study, a novel DNA biosensor was developed highly specific insertion sequence IS6110 M. tuberculosis, using reduced graphene oxide–gold nanoparticles (rGO–AuNPs) as sensing platform gold...
High-risk human papillomavirus oncoproteins E6 and E7 are the major etiological factors of cervical cancer but insufficient for malignant transformation cancer. Dysregulated alternative splicing, mainly ascribed to aberrant splicing factor levels activities, contributes most hallmarks. However, do regulate expression factors? Does acts as an "accomplice" E6E7 promote progression? Here, we identified that SRSF10, which promotes tumorigenesis cervix, was upregulated by via E2F1 transcriptional...
Despite the accessibility of ultrasound, clinical potential ultrasound-active theranostic agents has not been fully realized because it requires combining sufficient imaging contrast, high encapsulation efficiency, and ultrasound-triggered release in one entity. We report on polymer microcapsules composed hydrogen-bonded multilayers tannic acid poly(N-vinylpyrrolidone) that produce contrast deliver anticancer drug doxorubicin upon low-power diagnostic or high-power therapeutic ultrasound...
Fluorescent biosensors have been widely applied in DNA detection because of their reliability and reproducibility. However, low kinetics hybridization often brings out long test terms, thus restricting practical use. Here, we demonstrate unexpected fast fluorescence on the confined surface hydrogel particles. When pore size charge particles are tailored, molecules can be outer water layer We fabricated a fluorescence-on sensor based particle array by utilizing quenching property graphene...
A lysosome-targeting chimera (LYTAC) offers a novel strategy for degrading extracellular proteins previously considered to be undruggable by hijacking the lysosomal degradation system. However, clinical use of LYTAC has been limited due potential uncontrolled and systemic toxicity. Based on our developed genetically encoded TfR-LYTAC, here, we introduce photothermal-inducible switch in engineered bacterium enable spatiotemporal expression TfR-LYTAC. We also precise photothermal systems...
Group II introns and non-LTR retrotransposons encode a phylogenetically related family of highly processive reverse transcriptases (RTs) that are essential for mobility persistence these retroelements. Recent crystallographic studies on members this RT have revealed they structurally distinct from the retroviral RTs typically used in biotechnology. However, quantitative, structure-guided analysis processivity, efficiency, accuracy alternate has been lacking. Here, we characterize...
Metabolic syndrome caused obesity has long been recognized as a risk of health. Celery and celery extracts have various medicinal properties, such anti-diabetes anti-inflammatory properties blood glucose serum lipid reduction. However, the effect probiotic fermentation on juice association between fermented (FCJ) were unclear. This study aimed to evaluate beneficial effects FCJ high-fat diet (HFD) induced related metabolic syndromes. C57BL/6 mice randomly divided into six groups (n = 15 per...
Emerging coronaviruses (CoVs) pose a severe threat to human and animal health worldwide. To identify host factors required for CoV infection, we used α-CoV transmissible gastroenteritis virus (TGEV) as model genome-scale CRISPR knockout (KO) screening. Transmembrane protein 41B (TMEM41B) was found be bona fide factor involved in infection by three additional families. We that TMEM41B is critical the internalization early-stage replication of TGEV. Notably, our results also showed cells...
The aim of this study was to investigate the effects puerarin (Pue), a phytoestrogen, on production performance, egg quality, endocrine hormones, antioxidant capacity, and intestinal morphology in aged laying hens. A total 180 Hy-Line Brown hens 480 d were randomly divided into 4 groups (n = 45 per group) fed 0, 200, 400, 800 mg/kg Pue (Con, L-Pue, M-Pue, H-Pue, respectively) during 42-d experiment. Compared with Con treatment, supplementation H-Pue improved performance quality by...