Abstract Mesenchymal stem cells (MSCs) possess immunomodulatory properties and have potential, however, there been conflicting reports regarding their effects in rheumatoid arthritis (RA), which causes inflammation destruction of the joints. Through a comparative analysis regulatory T (Treg) IL-10-producing type 1 (Tr1) cells, we hypothesized that Tr1 enhance immunoregulatory functions MSCs, combinatorial approach to cell therapy may exert synergistic an experimental animal model (RA). A...

Graft-versus-host disease (GVHD) is a major complication associated with allogeneic hematopoietic stem cell transplantation. Despite the prominent role of adaptive immune system, importance controlling innate system in pathogenesis GVHD has recently been rediscovered. High-mobility group box 1 (HMGB1) crucial damage-associated molecular pattern signal that functions as potent mediator GVHD. In present study, we investigated treatment experimental through HMGB1 blockade using compound...

Mesenchymal stem cells (MSCs) have been considered to be an ideal cellular source for graft-versus-host disease (GVHD) treatment due their unique properties, including tissue repair and major histocompatibility complex (MHC)-unmatched immunosuppression. However, preclinical clinical data suggested that the immunomodulatory activity of MSCs is not as effective previously expected. This study was performed investigate whether capacity could enhanced by combination infusion regulatory T (Treg)...

Interleukin (IL)-21, a proinflammatory cytokine, has been developed as an immunotherapeutic approach due to its effects on various lymphocytes, including natural killer (NK) cells and T cells; however, the clinical success in cancer patients limited. Recently, mesenchymal stem (MSCs) have emerged vehicles for gene therapy their inherent migratory abilities toward tumors. In present study, we hypothesized that MSCs, genetically modified express high levels of IL-21 (IL-21/MSCs), can enhance...

Mesenchymal stem cells (MSCs) are an attractive tool to treat graft-versus-host disease because of their unique immunoregulatory properties. Although human bone marrow-derived MSCs (BM-MSCs) were the most widely used in cell therapy until recently, derived from umbilical cords (UC-MSCs) have gained popularity as material for ethical and noninvasive collection.To investigate difference mechanisms immunosuppressive effects UC-MSCs BM-MSCs.To analyze soluble factors expressed by MSCs, such...

Human bone marrow-derived mesenchymal stromal cells (BM-MSCs) have been proposed as a treatment for graft-versus-host disease (GVHD), which is major complication following allogeneic hematopoietic cell transplantation. However, clinical trials not yielded good results, and human decidua-derived (DSCs) an alternative. In addition, the mechanism by DSCs exert their immunomodulatory effects still unknown. We found that knockdown of IL-6 in reduced expression PD-L1 PD-L2, are known classical...

Therapeutic effects of combined cell therapy with mesenchymal stem cells (MSCs) and regulatory T (Treg cells) have recently been studied in acute graft-versus-host-disease (aGVHD) models. However, the underlying, seemingly synergistic mechanism behind has not determined. We investigated origin Foxp3+ Treg interleukin 17 (IL-17+) recipients following allogeneic bone marrow transplantation (allo-BMT) to identify immunological therapy. were generated from eGFP-expressing C57BL/6 mice (Tregegfp...

Establishment of mixed chimerism is an ideal approach to induce donor-specific tolerance while expanding its potential in various clinical settings. Despite the developments partial conditioning regimens, improvements are still needed reducing toxicity and bone marrow transplantation-related complications. Recently, cell-based therapies, including mesenchymal stem cells (MSCs), have been incorporated establishing noncytoreductive protocols; however, efficacy only shows reversed...

Oral mucositis (OM) is a common complication in cancer patients undergoing anticancer treatment. Despite the clinical and economic consequences of OM, there are no drugs available for its fundamental control. Here we show that high-mobility group box 1 (HMGB1), "danger signal" acts as potent innate immune mediator, plays critical role pathogenesis OM. In addition, investigated treatment OM through HMGB1 blockade using NecroX-7...

Chronic graft-versus-host disease (cGVHD) is a long-term complication of allogeneic hematopoietic stem cell transplantation associated with poor quality life and increased morbidity mortality. Currently, there are several approved treatments for patients who do not respond to steroids, such as ruxolitinib. Nevertheless, significant proportion fail second-line treatment, indicating the need novel approaches. Mesenchymal cells (MSCs) have been considered potential treatment approach...

Mesenchymal stromal cells (MSCs) are seen as an ideal source of to induce graft acceptance; however, some reports have shown that MSCs can be immunogenic rather than immunosuppressive. We speculate the immunomodulatory effects regulatory T (Tregs) aid maintenance immunoregulatory functions MSCs, and a combinatorial approach cell therapy synergistic on allograft rejection. After preconditioning with Fludarabine, followed by total body irradiation anti-asialo-GM-1(ASGM-1), tail skin grafts...

AIM:To investigate the effects of mesenchymal stem cells (MSCs) on dextran sulfate sodium-induced inflammatory bowel disease (IBD).METHODS: C57BL/6 mice were fed 3.5% (g/L) sodium.On day seven, received intraperitoneal injections 1 × 106 MSCs.The survival rate, activity index values, and body weight, monitored daily.On ten, colon lengths histopathologic changes assessed.In addition, immunoregulatory following MSC administration evaluated by determining levels effector T cell responses in...

Immunological characteristics of COVID-19 show pathological hyperinflammation associated with lymphopenia and dysfunctional T cell responses. These features provide a rationale for restoring functional immunity in patients by adoptive transfer SARS-CoV-2 specific cells.To generate cells, we isolated peripheral blood mononuclear cells from 7 recovered 13 unexposed donors. Consequently, stimulated peptide mixtures covering spike, membrane nucleocapsid proteins. Then, culture expanded IL-2 21...

Cytomegalovirus(CMV)-related diseases are a serious cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). CMV-specific cytotoxic T lymphocytes (CMV-CTLs) have been reported as an alternative to antiviral drugs that provide long-term immunity without major side effects. However, their application has limited by the prolonged manufacturing process required.

Graft-versus-host disease (GVHD) is a common complication following hematopoietic stem cell transplantation and can be life-threatening. Mesenchymal cells (MSCs), adult with immunomodulatory properties, have been used as therapeutic agents in variety of ways demonstrated efficacy against acute GVHD (aGVHD); however, variability MSC pro- anti-inflammatory properties the limitation that they only exhibit immunosuppressive effects at high levels inflammation prevented their widespread clinical...

Interleukin (IL)-10-producing type 1 regulatory T (Tr1) cells, which are Foxp3−memory lymphocytes, play important roles in peripheral immune tolerance. We investigated whether Tr1 cells exert immunoregulatory effects a mouse model of acute graft-versus-host disease (GVHD). Mouse CD4+ were induced to differentiate vitro into using vitamin D3 and dexamethasone, these donor-derived infused on the day bone marrow transplantation. The cell-transferred group showed less weight-loss twofold higher...

Introduction B-cell-activating factor (BAFF) is associated with donor-specific antibodies and chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, the effects of BAFF on T-cell physiological function have not been fully elucidated in acute GVHD. Methods We examined belimumab, a monoclonal antibody targeting BAFF, for treatment T cells B separately when inducing GVHD mouse model. Results Therapeutic functional manipulation...

Antigenic domain of major surface protein (p30) Toxoplasma gondii was analyzed after polymerase chain reaction (PCR) its gene fragments. Hydrophilic or hydrophobic moiety amino acid sequences were expressed as glutathione S-transferase (GST) fusion proteins. Fragments p30 follows: T37, total open reading frame (ORF); S28, ORF excluding N-terminal signal sequence and C-terminal sequence: A19, 2/3 parts S28; P19, X9, 1/3 part Y10, middle Z9, respectively. Primer each fragment synthesized to...

Background/Aims Adoptive therapy with regulatory T (Treg) cells to prevent graft-versus-host disease (GVHD) would benefit from a strategy improve homing the sites of inflammation following hematopoietic stem cell transplantation (HSCT). Although donor-derived Treg have mainly been used in these models, third-party-derived are promising alternative for cell-based immunotherapy, as they can be screened pathogens and activity, banked GVHD prevention. In this study, we explored major...

Abstract Immune checkpoint inhibitors have revolutionized anti-tumor therapy, notably improving treatment responses in various tumors. However, many patients remain non-responsive and do not experience benefits. Given that Toll-like receptors (TLRs) can counteract tumor immune tolerance by stimulating both innate adaptive responses, TLR agonists are being explored as potential adjuvants for cancer treatment. In this study, we assessed the of enhancing efficacy activating immunity with a TLR5...