A5055023793- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Immune responses and vaccinations
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Long-Term Effects of COVID-19
- HIV Research and Treatment
- Gut microbiota and health
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebrospinal fluid and hydrocephalus
- Neonatal and fetal brain pathology
- Epilepsy research and treatment
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Tuberculosis Research and Epidemiology
- Tryptophan and brain disorders
- HIV-related health complications and treatments
Tulane University
2018-2023
Gut dysbiosis is a common feature associated with the chronic inflammation of HIV infection. Toward understanding interplay treated infection, dysbiosis, and systemic inflammation, we investigated longitudinal fecal microbiome changes plasma inflammatory markers in nonhuman primate model. Following simian immunodeficiency virus (SIV) infection rhesus macaques, significant were observed several members phylum Firmicutes along an increase Bacteroidetes. Viral suppression antiretroviral therapy...
HIV-associated inflammation has been implicated in the premature aging and increased risk of age-associated comorbidities cART-treated individuals. However, immune mechanisms underlying chronic inflammatory state cART-suppressed HIV infection remain unclear. Here, we investigated role γδT cells, a group innate IL-17 producing T lymphocytes, development systemic leaky gut phenotype during SIV macaques. Plasma levels mediators, intestinal epithelial barrier disruption (IEBD) microbial...
The novel coronavirus SARS-CoV-2 emerged in late 2019, rapidly reached pandemic status, and has maintained global ubiquity through the emergence of variants concern. Efforts to develop animal models have mostly fallen short recapitulating severe disease, diminishing their utility for research focusing on disease pathogenesis life-saving medical countermeasures. We tested whether route experimental infection substantially changes COVID-19 characteristics two species nonhuman primates ( Macaca...
Abstract Although most SARS-CoV-2 infections are mild, some patients develop systemic inflammation and progress to acute respiratory distress syndrome (ARDS). However, the cellular mechanisms underlying this spectrum of disease remain unclear. γδT cells T lymphocyte subsets that have key roles in mucosal immune responses during infection inflammation. Here we show peripheral rapidly activated following aerosol or intra-tracheal/intra-nasal (IT/IN) nonhuman primates. Our results demonstrate a...
Background: Clinical measurements commonly used to evaluate overall health of laboratory animals including complete blood count, serum chemistry, weight, and immunophenotyping, differ with respect age, development, environment. This report provides comprehensive clinical immunological reference ranges for pediatric rhesus macaques over the first year life. Methods: We collected analyzed samples from 151 healthy macaques, aged 0-55 weeks, compared mother-reared infants two categories...
Abstract Aging is characterized by a loss of bone marrow hematopoietic tissue, systemic chronic inflammation, and higher susceptibility to infectious noninfectious diseases. We previously reported the tightly regulated kinetics massive daily production neutrophils during homeostasis in adult rhesus macaques aged 3 19 yr (equivalent approximately 10 70 age humans). In current study, we observed an earlier release recently dividing from greater in-group variability neutrophil based on vivo...
Dextrans have been used extensively as medical therapies and labeling agents in biomedical research to investigate the blood-brain barrier CSF flow absorption. Adverse effects from dextrans include anaphylactic reaction dilation of cerebral ventricles due administration into subarachnoid space. This retrospective study describes 51 rhesus macaques (Macaca mulatta) that received dextran intrathecally. The purpose intrathecal was enable detection long-lived, dextran-labeled macrophages...
Abstract Immunosenescence is a public health concern. This study was to characterize the peripheral immune system in rhesus macaques of varying ages. Flow cytometry and multiplex cytokine levels were evaluated blood from 35 monkeys (3.2-20 years old). Linear regression used define statistically significant changes (i.e. P<0.05) with age. Significant declines observed numbers neutrophils, eosinophils, basophils, lymphocytes increasing No monocytes, dendritic cells (DC), RBCs, or...
Abstract Aging is characterized with a loss of hematopoietic tissue in bone marrow, systemic chronic inflammation, and higher susceptibility to infectious diseases. We previously showed the tightly regulated kinetics massive daily production neutrophils during homeostasis rhesus macaques aged 3 19 years old. Here we extended that study further investigate effect aging on more elderly macaques. observed neutrophil shift in-group variability above 20 old (equivalent 70 or humans) by vivo BrdU...
Abstract The novel coronavirus SARS-CoV-2 has caused a worldwide pandemic resulting in widespread efforts development of animal models that recapitulate human disease for evaluation medical countermeasures, and to dissect COVID-19 immunopathogenesis. We tested whether route experimental infection substantially changes characteristics two species ( Macaca mulatta ; rhesus macaques; RM, Chlorocebus atheiops African green monkeys; AGM) nonhuman primates. Species-specific cohorts RM AGM Rhesus...
Background Although BCG vaccine protects infants from tuberculosis (TB), it has limited efficacy in adults against pulmonary TB. Further, HIV coinfection significantly increases the risk of developing active In lack defined correlates protection TB disease, is essential to explore immune responses beyond conventional CD4 T cells gain a better understanding mechanisms immunity. Methods Here, we evaluated unconventional lipid-reactive cell cynomolgus macaques following aerosol inoculation and...
Abstract Infection with SARS-CoV-2, the novel coronavirus responsible for current COVID-19 pandemic, results in a wide range of pathologies from asymptomatic/mild outcome to fulminant pneumonia and progression acute respiratory distress syndrome (ARDS). The cellular mechanisms underlying this spectrum disease remain unknown. We investigated dynamic changes frequency activation status peripheral innate lymphocyte subsets two different species nonhuman primates. Cohorts Rhesus macaques (n=8)...