A5086378954- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Microbial Metabolic Engineering and Bioproduction
- Carcinogens and Genotoxicity Assessment
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sarcoma Diagnosis and Treatment
- HIV/AIDS Research and Interventions
- Fermentation and Sensory Analysis
- Hemoglobinopathies and Related Disorders
- Immune cells in cancer
- Pharmacogenetics and Drug Metabolism
- Biochemical and biochemical processes
- Erythrocyte Function and Pathophysiology
- Drug-Induced Hepatotoxicity and Protection
- Heavy Metal Exposure and Toxicity
- HIV-related health complications and treatments
- Meat and Animal Product Quality
- Monoclonal and Polyclonal Antibodies Research
- Chromium effects and bioremediation
- Biochemical effects in animals
- Heme Oxygenase-1 and Carbon Monoxide
Kumamoto University
2021-2024
Yamagata University Hospital
2023
Society of Environmental Toxicology and Chemistry
2010-2021
Tulane University
2019-2021
National Institute of Infectious Diseases
2012-2018
National Institutes of Health
2014
The University of Tokyo
2012-2013
Gifu University
2002-2005
The Nisshin OilliO Group (Japan)
2000
Iwate Medical University
2000
ABSTRACT Nonhuman primate AIDS models are essential for the analysis of pathogenesis and evaluation vaccine efficacy. Multiple studies on human immunodeficiency virus simian (SIV) infection have indicated association major histocompatibility complex class I (MHC-I) genotypes with rapid or slow progression. The accumulation macaque groups that share not only a single MHC-I allele but also an haplotype consisting multiple polymorphic loci would greatly contribute to progress research. Here, we...
For development of an effective T cell-based AIDS vaccine, it is critical to define the antigens that elicit most potent responses. Recent studies have suggested Gag-specific and possibly Vif/Nef-specific CD8(+) cells can be important in control virus. Here, we tested whether induction these by prophylactic vaccination result simian immunodeficiency virus (SIV) replication Burmese rhesus macaques sharing major histocompatibility complex class I (MHC-I) haplotype 90-010-Ie associated with...
ABSTRACT Identifying human immunodeficiency virus type 1 (HIV-1) control mechanisms by neutralizing antibodies (NAbs) is critical for anti-HIV-1 strategies. Recent in vivo studies on animals infected with simian (SIV) and related viruses have shown the efficacy of postinfection NAb passive immunization viremia reduction, one suggested mechanism its occurrence through modulation cellular immune responses. Here, we describe SIV macaques showing biphasic CD8 + cytotoxic T lymphocyte (CTL)...
ABSTRACT CD4 + T-cell responses are crucial for effective antibody and CD8 induction following virus infection. However, virus-specific T cells can be preferential targets human immunodeficiency (HIV) HIV-specific by vaccination may thus result in enhancement of replication In the present study, we show that vaccine-elicited expressing CD107a relatively resistant to depletion a macaque AIDS model. Comparison CD107a, macrophage inflammatory protein-1β, gamma interferon, tumor necrosis factor...
The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare experiments. Herein, we briefly discuss the current status hepatoxicity human- and rat-derived hepatocytes; focus on liver organoid method, which been extensively studied recent years, how toxicologic pathologists can use their knowledge experience to contribute chemical hepatotoxicity drugs, pesticides, chemicals. We also propose toxicological should assess...
In a past study, we proposed modified Comparative Thyroid Assay (CTA) with additional examinations of brain thyroid hormone (TH) concentrations and histopathology but smaller group sizes. The results showed that the CTA in Sprague Dawley rats detected 10 ppm 6-propylthiouracil (6-PTU)-induced significant suppressions serum/brain TH offspring. To confirm reliability qualitative identify optimal testing time for heterotopia (a cluster ectopic neurons) CTA, together were assessed GD20 fetuses...
Abstract Despite effective antiretroviral therapy, HIV-1 persists in cells, including macrophages, which is an obstacle to cure. However, the precise role of macrophages infection remains unclear because they reside tissues that are not easily accessible. Monocyte-derived widely used as a model peripheral blood monocytes cultured and differentiated into macrophages. another needed recent studies revealed most adult originate from yolk sac fetal liver precursors rather than monocytes,...
Didecyldimethylammonium chloride (DDAC), a representative dialkyl-quaternary ammonium compound (QAC), could contaminate working atmospheres when used in disinfectant operation and adversely affect human health. Furthermore, the development of bacteria resistant to DDAC might become public health concern. We postulated that instillation lungs alters pulmonary antioxidant antimicrobial responses increases susceptibility systemic administration bacterial component lipopolysaccharide (LPS). Mice...
Aldehyde oxidase (aldehyde: oxygen oxidoreductase, EC 1.2.3.1) was partially purified from bovine liver. The enzyme irreversibly oxidized various aldehydes to the corresponding acids by using dissolved as an electron acceptor. Although Km value for n-hexanal low (6μM), that acetaldehyde high (20mM). Medium-chain such hexanal and pentanal appear be mainly responsible green beany odor of soybean products. A great reduction in observed after extract incubated with aldehyde under aerobic...
Aldehyde dehydrogenase catalyzes the irreversible conversion of aldehydes into their corresponding acids. NAD-dependent aldehyde purified from bovine liver mitochondria was used to remove green beany flavor soybean products. Incubation enzyme, in presence NAD+, with defatted extracts or milk, resulted almost complete disappearance a great reduction flavor. It found experiments pyrazole, an inhibitor alcohol dehydrogenase, used, that alcohols contributing were converted acids by cooperative...
Several major histocompatibility complex class I (MHC-I) alleles are associated with lower viral loads and slower disease progression in human immunodeficiency virus (HIV) simian (SIV) infections. Immune-correlates analyses these MHC-I-related HIV/SIV controllers would lead to elucidation of the mechanism for control. Viral control some protective MHC-I is attributed CD8+ T-cell responses targeting Gag epitopes. We have been trying know SIV multiple groups Burmese rhesus macaques sharing...
Various physiological roles of mammalian aldehyde dehydrogenase had been anticipated because its broad substrate specificity. In order to clarify the enzyme and regulation metabolisms in liver, intracellular distribution isozyme beef liver were studied. The presence mitochondrial, microsomal cytoplasmic isozymes proved by isoelectric focusing. These different from each other pH-activity curve responces for steroid hormones disulfiram. It was suggested comparing reactivities these various...
Abstract The intestinal tract is a primary barrier to invading pathogens and contains immune cells, including lymphocytes macrophages. We previously reported that CD163+CD206− (single-positive [SP]) interstitial macrophages of the lung are short-lived succumb early SIV infection. Conversely, CD163+CD206+ (double-positive [DP]) alveolar long-lived, survive after infection, may contribute virus reservoir. This report characterizes analogous populations in rhesus macaques (Macaca mulatta) with...
Abstract HIV-1 persists in cellular reservoirs despite effective combined antiretroviral therapy (cART). CD4 + T cells are a well-known reservoir, but there is evidence suggesting that myeloid cells, including circulating monocytes, also clinically relevant reservoir. However, it not fully understood which subsets of monocytes preferentially infected vivo. Here, we show monocyte fraction expressing stem cell marker CD34 more susceptible to infection than the CD34-negative major subset. In...
The carbohydrate structure of sialyl-Lewis X (SLex) can function as a ligand for E- and P-selectin, which play important roles in mediating the initial interactions leukocytes with endothelium inflammatory responses. In this study we evaluated effects inhibiting P-selectin SLex molecule on response an experimental murine model hypersensitivity pneumonitis (HP). Antigen exposure induced marked interstitial especially perivascular peribronchiolar infiltration lymphocytes granuloma formation,...
Aldehyde dehydrogenase catalyzes the irreversible conversion of aldehydes into their corresponding acids. NAD-dependent aldehyde purified from bovine liver mitochondria was used to remove green beany flavor soybean products. Incubation enzyme, in presence NAD+, with defatted extracts or milk, resulted almost complete disappearance a great reduction flavor. It found experiments pyrazole, an inhíbitor alcohol dehydrogenase, used, that alcohols contributing were converted acids by cooperative...