A5069498173- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Chemical Synthesis and Analysis
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS Research and Interventions
- RNA and protein synthesis mechanisms
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Pharmacological Effects of Natural Compounds
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- Herpesvirus Infections and Treatments
- Hepatitis C virus research
- Plant biochemistry and biosynthesis
- Material Properties and Processing
- Radiomics and Machine Learning in Medical Imaging
- Chromatography in Natural Products
- Peripheral Neuropathies and Disorders
- Poxvirus research and outbreaks
National Institute of Infectious Diseases
2014-2024
Infectious Disease Research Institute
2024
National Cancer Institute
2003-2019
Inova Health System
2019
Georgetown University
2019
Moffitt Cancer Center
2019
Quantum Leap Healthcare Collaborative
2019
Sysmex (Japan)
2017
Kumamoto University
2002-2014
Academy of Medical Sciences
2012
Effective vaccines are essential for the control of coronavirus disease 2019 (COVID-19) pandemic. Currently developed inducing severe acute respiratory syndrome 2 (SARS-CoV-2) spike (S)-antigen-specific neutralizing antibodies (NAbs) effective, but appearance NAb-resistant S variant viruses is great concern. A vaccine S-independent or NAb-independent SARS-CoV-2 may contribute to containment these variants. Here, we investigate efficacy an intranasal expressing viral non-S antigens against...
BackgroundVaccination against mpox (formerly known as monkeypox), an infectious disease caused by the monkeypox virus (MPXV), is needed to prevent outbreaks and consequent public health concerns. The LC16m8 vaccine, a dried cell-cultured proliferative live attenuated vaccinia virus–based was approved in Japan smallpox mpox. However, its immunogenicity efficacy MPXV have not been fully assessed. We assessed safety of healthy adults.MethodsWe conducted single-arm study that included 50...
N-(4-Chlorophenyl)-N'-(2,2,6,6-tetramethyl-piperidin-4-yl)-oxalamide (NBD-556) is a low-molecular-weight compound that reportedly blocks the interaction between human immunodeficiency virus type 1 (HIV-1) gp120 and its receptor CD4. We investigated whether enhancement of binding anti-gp120 monoclonal antibodies (MAbs) toward envelope (Env) protein with NBD-556 are similar to those soluble CD4 (sCD4) by comparing profiles individual MAbs Env-expressing cell surfaces. In flow cytometric...
Abstract CD4 mimics are small molecules that inhibit the protein–protein interaction between gp120 and CD4, which is a key for entry of human immunodeficiency virus (HIV) into host immune cells. In present study, mono‐cyclohexyl‐type were designed to form hydrophobic electrostatic interactions with Val430 Asp368 located in entrance Phe43 cavity gp120, site CD4. YIR‐329, novel 1‐azaspiro[5.5]undecane derivative cyclohexyl ring attached piperidine ring, exhibited only slightly weaker anti‐HIV...
ABSTRACT Identifying human immunodeficiency virus type 1 (HIV-1) control mechanisms by neutralizing antibodies (NAbs) is critical for anti-HIV-1 strategies. Recent in vivo studies on animals infected with simian (SIV) and related viruses have shown the efficacy of postinfection NAb passive immunization viremia reduction, one suggested mechanism its occurrence through modulation cellular immune responses. Here, we describe SIV macaques showing biphasic CD8 + cytotoxic T lymphocyte (CTL)...
The stimulatory effects of several DNA-binding basic proteins (histone and protamine) HIV-1 Rev with arginine (Arg)-rich clusters on the activity casein kinase II (CK-II) were investigated in vitro. It was found that recombinant (rRev) synthetic oligo-fragments corresponding to amino acid sequences its Arg-rich cluster stimulate CK-II a dose-dependent manner. activated phosphorylates cellular viral infected human MOLT-4 cells, also structural proteins, including reverse transcriptase (rRT)....
The physiological significance of the casein kinase II (CK-II)-mediated phosphorylation human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) on its three enzymatic activities [RNA-dependent DNA polymerase (RDDP), DNA-dependent (DDDP) and ribonuclease H (RNase H)] was investigated in vitro. It found that (i) purified recombinant RT (rRT) functioned as an effective phosphate acceptor for CK-II; (ii) RDDP, DDDP RNase activity rRT stimulated about 2.8-, 4.1- 3.9-fold,...
The aim of this study was to generate maraviroc (MVC)-resistant viruses in vitro using a human immunodeficiency virus type 1 subtype B clinical isolate (HIV-1KP-5) understand the mechanism(s) resistance MVC. To select HIV-1 variants resistant MVC vitro, we exposed high-chemokine (C-C motif) receptor 5 (CCR5)-expressing PM1/CCR5 cells HIV-1KP-5 followed by serial passage presence We also passaged PM1 cells, which were low CCR5 expressing determine low-CCR5-adapted substitutions and compared...
Objective: To investigate the mechanism by which Q151M mutation in reverse transcriptase (RT) that confers multi-dideoxynucleoside resistance on HIV-1 and requires a two base change (CAG←ATG) develops, to understand reason for relatively lengthy period of time required its emergence under therapy with multiple nucleoside RT inhibitors (NRTI). Design methods: Propagation assays competitive replication were used evaluate fitness various infectious clones, including putative intermediates...
Fibrocytes (fibroblastic leukocytes) are recently identified as unique hematopoietic cells with features of both macrophages and fibroblasts. known to contribute the remodeling or fibrosis various injured tissues. However, their role in viral infection is not fully understood. In this study, we show that differentiated fibrocytes phenotypically distinguishable from but can be infected HIV-1. Importantly, exhibited persistently cell-like phenotypes, degree which was more apparent than...
CD4 mimics are small molecules that inhibit the interaction of gp120 with CD4. We have developed several mimics. Herein, hybrid consisting a long alkyl chain or PEG unit attached through self-cleavable linker were synthesized. In anti-HIV activity, modification appeared to be more suitable than chain. Thus, mimics, units an uncleavable linker, and showed high activity low cytotoxicity. investigation pharmacokinetics in rhesus macaque, compound had effective PK profile parent compound,...
In an attempt to determine which amino acid(s) of LD78beta, a variant human macrophage inflammatory protein-1alpha, plays critical role in the interaction with CCR5, we generated six LD78beta variants acid substituted Ala at NH(2) terminus LD78beta. There was no significant difference eliciting Ca(2+) flux and chemotaxis among exception LD78beta(T9A) showing substantially reduced activity. The comparative order for immunodeficiency virus type 1 (HIV-1) replication inhibition was:...
SARS-CoV-2 infection presents clinical manifestations ranging from asymptomatic to fatal respiratory failure. Despite the induction of functional SARS-CoV-2-specific CD8 + T-cell responses in convalescent individuals, role virus-specific control replication remains unknown. In present study, we show that subacute can be controlled absence T cells cynomolgus macaques. Eight macaques were intranasally inoculated with 10 5 or 6 TCID 50 SARS-CoV-2, and three eight treated a monoclonal anti-CD8...