A5015733102- Cancer Immunotherapy and Biomarkers
- Asthma and respiratory diseases
- Immune cells in cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunotherapy and Immune Responses
- Liver Disease Diagnosis and Treatment
- Eosinophilic Esophagitis
- Systemic Sclerosis and Related Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bariatric Surgery and Outcomes
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Immune Cell Function and Interaction
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory and Cough-Related Research
- Sepsis Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Cancer Cells and Metastasis
- Cutaneous lymphoproliferative disorders research
- Blood disorders and treatments
- Lymphatic System and Diseases
- Mast cells and histamine
- Platelet Disorders and Treatments
- Extracellular vesicles in disease
- Sarcoidosis and Beryllium Toxicity Research
Institut de Recerca Sant Pau
2024
Hospital de Sant Pau
2017-2024
Universitat Autònoma de Barcelona
2024
Abstract The migration and antimicrobial functions of neutrophils seem to be impaired during sepsis contribute the dysregulation immune responses disease pathogenesis. However, role neutrophil extracellular traps (NETs) remains clarified. study aimed analyse sequential phenotypic functional changes time following diagnosis sepsis. We prospectively enrolled 49 septic 18 non‐septic patients from intensive care unit (ICU) emergency room (ER) 20 healthy volunteers (HV). Baseline blood samples...
Background: Platelets (PLTs) from healthy donors (HD) modulate T lymphocyte respons-es but PLTs rheumatoid arthritis (RA) patients contribute to persistent systemic in-flammation. This suggests that RA and HD have different immuno-modulatory effects. Methods: Using cell culture, flow cytometry, proteomics, ELISA, we compared their effects on activation cytokine pro-duction. Results: suppressed proliferation IFNγ TNF production, while exhibited reduced suppressive capacity. In the presence of...
Immune checkpoint inhibitors (ICI) have the potential to trigger unpredictable immune-related adverse events (irAEs), which can be severe. The underlying mechanisms of these are not fully understood. As PD-L1 is upregulated by IFN, heightened immune activation resulting from PD-1/PD-L1 inhibition may enhance IFN response, triggering expression IFN-inducible genes and contributing irAE development its severity. In this study, we investigated interplay between irAEs chemokines cytokines in 134...
PD-(L)1 inhibitors are part of the treatment strategy for non-small cell lung cancer (NSCLC) although its efficacy is limited to certain patients. Our study aimed identify patients who might benefit from anti-PD-(L)1 by analyzing PD-L1 expression on circulating leukocytes and evolution during treatment. One hundred thirteen NSCLC patients, according their radiological response after 10–12 weeks treatment, were classified into responders, stable, progressive disease. Percentages PD-L1+...
Background and Aims: Alcohol use disorder has been reported in patients undergoing bariatric procedures, but the pattern of alcohol consumption not evaluated. We investigated prevalence, risk factors, impact binge drinking (BD) at time surgery during follow-up. Methods: A prospective, longitudinal study subjects was included LABS-2 registry between 2006 2009. Participants with AUDIT questionnaire a minimum 12 months follow-up were included. BD defined as consuming ≥5 drinks on least 1...
Cytotoxic T lymphocyte (CTLs) activation is an independent predictor of response to neoadjuvant chemotherapy (NACT) in breast cancer (BC) patients. Here, we go deeper into the function CD8+ HLA-DR+ cells from NACT treated HER2 negative BC Flow cytometry analysis revealed that cell percentage was increased responder (R) compared non-responder (NR) R patients with ER-/PR- hormone receptors had highest frequencies, while no differences were found when classified according stage or menopause...
Two subsets of eosinophils have been described: resident with homeostatic functions (rEOS) in healthy subjects and patients nonallergic eosinophilic asthma, inflammatory (iEOS) blood lung samples from allergic asthma. We explored if it would be possible to identify different using flow cytometry the gating strategy applied induced sputum. conducted an observational cross-sectional single-center study 62 persistent Inflammatory cells sputum were counted by light microscopy cytometry, cytokine...
Currently, therapy response cannot be accurately predicted in HER2-negative breast cancer (BC). Measuring stromal tumour-infiltrating lymphocytes (sTILs) and mediators of the tumour microenvironment characterizing immune cells (TIICs) may improve treatment neoadjuvant setting. Tumour tissue peripheral blood samples were retrospectively collected from 118 patients, sTILs evaluated. Circulating exosomes myeloid-derived suppressor determined by flow cytometry. TIICs markers (CD4, CD8, CD20,...
Summary Eltrombopag (ELT) is a thrombopoietin‐receptor agonist that stimulates platelet (PLT) production in patients with primary immune thrombocytopenia (ITP). One potential mechanism of ELT modulating the inflammatory response by increasing PLTs binding to leucocytes. This study examined effect on leucocyte–PLTs complexes 38 ITP patients. Patients, predominantly females mean age 59 years, underwent treatments like corticosteroids, intravenous immunoglobulin and splenectomy. Compared...
Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety cells. Our goal aimed investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), sarcoidosis hypersensitivity pneumonitis (HP) cohorts. ILD patients followed at Siena, Barcelona Foggia University Hospitals were enrolled. BAL supernatants used isolate the EVs. They characterized flow...
Pulmonary fibrosis involves various types of immune cells and soluble mediators, including TGF-β IL-35, a recently identified heterodimeric cytokine that belongs to the IL-12 family. However, effect regulatory IL-35 may play an important role in fibrotic diseases. The aim this paper is explore immunoregulatory development interstitial lung disease (ILD). To gain better understanding issue, concentrations different profibrotic cytokines (F-ILD) non-fibrotic (NF-ILD) patients by ELISA were...
This study investigated the role of IL-35 in systemic sclerosis (SSc) patients, focusing on CD4+ T cell response and immunomodulatory cytokine production. By comparing levels healthy donors (HD) SSc patients using ELISAs, we found a significantly lower plasma concentration (52.1 ± 5.6 vs. 143 11.1, p < 0.001). Notably, showed negative correlation with TGF-β (p 0.001) IL-17 = 0.04). Assessing IL-35R expression across types HDs via flow cytometry, higher monocytes (40.7 + 5.7 20.3 1.9, CD8+...
Malignant pleural effusion (MPE) is a common severe complication of advanced lung adenocarcinoma (LAC). Neutrophils, an essential component tumor infiltrates, contribute to progression and their counts in MPE have been associated with worse outcome LAC. This study aimed evaluate phenotypical functional changes neutrophils induced by determine the influence immunomodulatory factors neutrophil response find possible association between functions clinical outcomes. Pleural fluid samples were...
Interstitial lung diseases (ILDs) are characterized by inflammation or fibrosis of the pulmonary parenchyma. Despite involvement immune cells and soluble mediators in fibrosis, influence antimicrobial peptides (AMPs) remains underexplored. These effector molecules display a range activities, which include immunomodulation wound repair. Here, we investigate role AMPs development ILD. We compare concentration different cytokines 46 fibrotic (F-ILD) 17 non-fibrotic (NF-ILD) patients ELISA using...
Advanced chronic liver disease (ACLD) is associated with a wide spectrum of immune dysfunction. The clinical impact SARS-CoV-2 on the development decompensation and response in unvaccinated outpatients has not as yet been clearly defined. This study aimed to evaluate immunological ACLD. an observational case–control study, which ACLD were included prospectively consecutively classified into two groups: infected non-infected. Patients’ baseline characteristics infection data collected...
<b>Introduction:</b> Immunoregulatory end effector eosinophil subpopulations, the latter capable of antigen presentation and CD4+ T cell activation, have been identified in mouse. Functionally different subpopulations may infiltrate airways asthma be involved varying phenoendotypic mechanisms, not yet humans. <b>Aims:</b> To explore phenotype variants asthmatic airways, potential value for new phenoendotyping precision diagnostics. <b>Material methods:</b> 21 asthmatics were enrolled (13...
<b>Aims and objectives:</b> Our aim is to investigate the profile of antimicrobial peptides in bronchoalveolar lavage patients with ILD. <b>Methods:</b> Bronchoalveolar (BAL) fluids were obtained from ILD's stored at -20ºC. Depending on presence fibrotic findings High resolution computerized tomography (HRCT) scan, they classified as vs non-fibrotic. Demographic clinical parameters collected. Levels Lactoferrin, Secretory Leukocyte Peptidase Inhibitor (SLPI) Lysozyme measured BAL samples...
<b>Background:</b> Antigen sensitization is conditioned by factors locally regulating the immune response. SEB a superantigen with an attributed role in human to aeroallergens. In rat, asthma has been chiefly modeled injected ovalbumin employing Brown Norway (BN) T-helper-2 (Th2) biased strain. The reservoir of optimal SsN-UK BN substrain recently lost. <b>Aims and objectives:</b> We aimed at alternative rat model that would more closely reflect pathophysiology allergic sensitization....
<b>Background:</b> The usual atopic asthma rat model, based on sensitization to injected ovalbumin with adjuvants, needs innovation. <b>Aims and objectives:</b> data presented here arose from an attempt establish a model intranasal (i.n.) <i>Dermatophagoides pteronyssinus</i>, more closely resemble pathophysiology. <b>Methods:</b> Brown Norway (BN)/Crl rats (Germany) were i.n. administered <i>D. pteronyssinus</i> (25 mcg/mL DerP1), 3 times weekly for 6 weeks (house dust mite [HDM] group,...
<b>Introduction:</b> Antigen-specific CD4+ T cells induce airway smooth muscle (ASM) remodeling through direct contact. Mesenchymal stem (MSCs) may offer therapeutic potential for asthma but it is necessary to understand their immunomodulatory mechanisms and mediators. <b>Aims objectives:</b> To test if MSCs, whether contact or mediators, inhibit ASM cell proliferation in a cell/ASM co-culture model. <b>Methods:</b> Sprague-Dawley rats were intranasally exposed D. pteronyssinus (HDM) with...
Despite the improvement in therapy most of non-Hodgkin lymphomas, scenario for advanced-stage mycosis fungoides/Sezary syndrome remains unmodified.Allogeneic stem cell transplantation is still best option to maintain a durable response, but it requires previous good control disease, and associated with several complications which could be related therapies.Here we report clinical case that pictures all critical aspects this rare aggressive lymphoma: diagnosis classification, selection...