A5072620032- Head and Neck Cancer Studies
- Lung Cancer Treatments and Mutations
- Head and Neck Surgical Oncology
- Mass Spectrometry Techniques and Applications
- Lung Cancer Diagnosis and Treatment
- Ear and Head Tumors
- Prostate Cancer Treatment and Research
- Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Advanced Radiotherapy Techniques
- Sinusitis and nasal conditions
- Radiomics and Machine Learning in Medical Imaging
- Advanced Proteomics Techniques and Applications
- Oral and Maxillofacial Pathology
- Cholesterol and Lipid Metabolism
- Esophageal Cancer Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Oral health in cancer treatment
- Cancer-related molecular mechanisms research
- Tumors and Oncological Cases
- Medical Imaging Techniques and Applications
- Cryospheric studies and observations
- Immune cells in cancer
Aalborg University Hospital
2009-2024
Norwegian University of Science and Technology
2017-2024
St Olav's University Hospital
2024
NTNU Samfunnsforskning
2022
China Medical University
2019
China Medical University Hospital
2019
Frederiksberg Hospital
2014
Aalborg University
2013
Moelven (Norway)
2007
Odense University Hospital
1988
Abstract Background Prostate cancer tissues are inherently heterogeneous, which presents a challenge for metabolic profiling using traditional bulk analysis methods that produce an averaged profile. The aim of this study was therefore to spatially detect metabolites and lipids on prostate tissue sections by mass spectrometry imaging (MSI), method facilitates molecular heterogeneous sections, can subsequently be related the histology same section. Methods Here, we simultaneously obtained...
Abstract Reactive stroma is a tissue feature commonly observed in the tumor microenvironment of prostate cancer and has previously been associated with more aggressive tumors. The aim this study was to detect differentially expressed genes metabolites according reactive content measured on exact same sample. evaluated using histopathology from 108 fresh frozen samples gathered 43 patients after prostatectomy (Biobank1). A subset analyzed both for metabolic (n = 85) transcriptomic alterations...
AbstractThe present study comprises 847 women operated upon for invasive breast carcinoma at 19 surgical departments and enrolled in protocol DBCG-82TM from January 1983 to November 1987. Among them 662 (78%) were allocated breast-preserving therapy or mastectomy by randomization, while 185 patients (22%) did not accept randomization. Within the randomized group 6% could be entered into adjuvant protocols, i.e. subsequent programmes of postoperative follow-up. This left 619 evaluable...
Objective. To evaluate the treatment outcome for sino-nasal carcinomas in Denmark from 1995–2004 and compare results to previous Danish survey covering 1982–1991. Design. Retrospective follow-up. Materials methods. In five head neck oncology centres, charts of all consecutive patients with were reviewed data extracted a common database. Altogether 242 period identified. Of these 162 (67%) male 80 (33%) female. Histologies included squamous cell carcinoma (55%), adenocarcinoma (28.5%),...
MALDI MS imaging (MSI) is a powerful analytical tool for spatial peptide detection in heterogeneous tissues. Proper sample preparation crucial to achieve high quality, reproducible measurements. Here we developed an optimized protocol spatially resolved proteolytic with time-of-flight MSI of fresh frozen prostate tissue sections. The parameters tested included four different washes, methods protein denaturation, trypsin digestion (different densities, sprayers, and incubation times), five...
Reliable and accessible biomarkers for patients with Head Neck Squamous Cell Carcinoma (HNSCC) are warranted biologically driven radiotherapy (RT). This study aimed to investigate the prognostic value of putative cancer stem cell (CSC) markers, hypoxia, tumor volume using loco-regional high-dose failure (HDF) as endpoint.
Abstract Prostate cancer treatment resistance is a significant challenge facing the field. Genomic and transcriptomic profiling have partially elucidated mechanisms through which cells escape treatment, but their relation toward tumor microenvironment (TME) remains elusive. Here we present comprehensive landscape of prostate TME at multiple points in standard timeline employing single-cell RNA-sequencing spatial transcriptomics data from 120 patients. We identify club-like as key epithelial...
Levels of zinc, along with its mechanistically related metabolites citrate and aspartate, are widely reported as reduced in prostate cancer compared to healthy tissue therefore pointed out potential biomarkers. Previously, it has only been possible analyze zinc by separate detection methods. Through matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI), we were for the first time able demonstrate, two different sample sets (n = 45 n 4), simultaneous spatial form ZnCl3–,...
Abstract To truly understand the cancer biology of heterogenous tumors in context precision medicine, it is crucial to use analytical methodology capable capturing complexities multiple omics levels, as well spatial heterogeneity tissue. Different molecular imaging techniques, such mass spectrometry (MSI) and transcriptomics (ST) achieve this goal by spatially detecting metabolites mRNA, respectively. take full advantage multi-omics data, individual measurements need be integrated into one...
Background. The post-treatment follow-up is well-integrated in the oncologic care tradition, based on risk of developing recurrent disease or new primary tumors treated patients. Furthermore, serves as an opportunity to monitor treatment effects and provide clinical side effects. In this study we measured activity effectiveness routine head neck cancer assessed value from perspectives both physicians Patients methods. During a period six weeks prospective national cross section cohort 619...
Introduction: Prediction models using logistic regression may perform poorly in external patient cohorts. However, there is a need to standardize and validate for clinical use. The purpose of this project was describe method validation NTCP used selection the randomized trial protons versus photons head neck cancer radiotherapy, DAHANCA 35.Material methods: Organs at risk 588 patients treated primarily with IMRT controlled DAHANCA19 were retrospectively contoured according recent...
Abstract Background Truly understanding the cancer biology of heterogeneous tumors in precision medicine requires capturing complexities multiple omics levels and spatial heterogeneity tissue. Techniques like mass spectrometry imaging (MSI) transcriptomics (ST) achieve this by spatially detecting metabolites RNA but are often applied to serial sections. To fully leverage advantage such multi-omics data, individual measurements need be integrated into 1 dataset. Results We present Multi-Omics...
Background: The study aimed to evaluate Hyperfractionated, Accelerated Radiotherapy (HART) with nimorazole for patients head and neck squamous cell carcinoma (HNSCC) using loco-regional failure (LRF), overall survival (OS), early late morbidity as endpoints.Material methods: From February 2007 January 2018, 295 unresected HNSCC, T1-T4, N0-N3, M0, were treated HART prescribed 76 Gy in 56 fractions (fx), 10 fx weekly. IMRT was used >90% of patients. No chemotherapy given. Patients...
AbstractBackground In the Danish Head and Neck Cancer Group (DAHANCA) 35 trial, patients are selected for proton treatment based on simulated reductions of Normal Tissue Complication Probability (NTCP) compared to photon at referring departments. After inclusion in immobilization, scanning, contouring planning repeated national centre. The new contours could result reduced expected NTCP gain plan, resulting a loss validity selection process. present study evaluates if contour consistency can...
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IntroductionPatients with failure after primary radiotherapy (RT) for head and neck squamous cell carcinoma (HNSCC) have a poor prognosis. This study investigates pattern of curatively intended IMRT in randomized controlled trial relation to HPV/p16 status.Material methodsPatients HNSCC the oral cavity, oropharynx (OPSCC), hypopharynx or larynx were treated curative (±cisplatin) concomitant nimorazole between 2007 12. Of 608 patients, 151 had loco-regional within five years, from whom 130...
Prostate tumor heterogeneity is a major obstacle when studying the biological mechanisms of molecular markers. Increased gene expression levels secreted frizzled-related protein 4 (SFRP4) biomarker in aggressive prostate cancer. To understand how SFRP4 relates to cancer we performed comprehensive spatial and multiomics analysis same tissue samples. The experimental workflow included transcriptomics, bulk proteomics, DNA methylomics staining. mRNA was predominantly located stroma, produced by...
Increased knowledge of the molecular differences between indolent and aggressive prostate cancer is needed for improved risk stratification treatment selection. Secreted frizzled-related protein 4 (SFRP4) a modulator cancer-associated Wnt pathway, previously suggested as potential marker aggressiveness. In this study, we investigated validated association SFRP4 gene expression aggressiveness in nine independent cohorts (n = 2157). By differential combined meta-analysis all cohorts, detected...
Altered metabolism is one of the key molecular characteristics prostate cancer (PCa) development, and a number studies have searched for metabolic biomarkers in patient-derived samples. Reported changes PCa tissue compared with normal include reduced levels citrate spermine, elevated lipid synthesis fatty acid oxidation, higher amino acids alanine, glutamine, glutamate. Recent investigated tumor microenvironment, such as reactive stroma, so-called field effects. Furthermore, analysis blood...